乳与乳制品中黄曲霉毒素检测研究进展

doi:10.16431/j.cnki.1671-7236.2022.07.041

摘要/Abstract

摘要： 黄曲霉毒素（aflatoxin，AF）是霉菌产生的毒性极强的次级代谢产物，主要存在于乳与乳制品和霉变谷物中。目前研究较多的黄曲霉毒素有黄曲霉毒素B₁（AFB₁）、AFB₂、AFG₁、AFG₂、AFM₁和AFM₂，黄曲霉毒素物理与化学性质稳定，加热处理很难将其破坏，是目前公认的毒性最强的致癌物之一。黄曲霉毒素不仅会造成动物营养不良及免疫力下降等问题，进入人体后还会对人体的肝脏造成极大的损害，而且原料乳中黄曲霉毒素的存在会对整个乳制品产业链造成严重的危害。因此，建立简便的、特异性好、灵敏度高的检测乳与乳制品中黄曲霉毒素的方法具有十分重要的意义。作者综述了乳与乳制品中黄曲霉毒素的检测方法进展，介绍了国内外近年来质谱法、电化学法、光谱法、色谱法、试纸条法等方法在检测乳与乳制品中黄曲霉毒素的应用，总结以上各种方法在检测乳与乳制品中黄曲霉毒素中的优势与其面临的挑战，并对今后乳与乳制品中黄曲霉毒素检测方法的发展方向进行了展望，以期为设计新的乳与乳制品中黄曲霉毒素检测方法提供思路。

关键词: 乳; 乳制品; 黄曲霉毒素; 研究进展

Abstract: Aflatoxins (AF) are secondary metabolites of Aspergillus species with hightoxicity,and mainly found in milk and dairy products and mouldy cereals.At present,there are a lot of studies on aflatoxin B₁ (AFB₁),AFB₂,AFG₁,AFG₂,AFM₁ and AFM₂ among all aflatoxins.The physical and chemical properties of aflatoxins are relatively stable,it is difficult to destroy aflatoxins by heating,and aflatoxins are currently recognised as one of the most toxic carcinogens.Aflatoxins not only cause nutritional problems such as malnutrition and reduced immunity to animals,but also cause great damage to the human liver when entering the body,and the presence of aflatoxins in raw milk causes serious harm to the whole dairy industry chain.Therefore,there is an urgent need to implement selective,sensitive and highly convenient methods for the determination of aflatoxins in milk and dairy products.This paper introduces the research progress of aflatoxins detection methods in milk and dairy products at home and abroad,summarizes the application of mass spectrometry,electrochemistry,spectroscopy,chromatography and test strips in the detection of aflatoxins in milk and dairy products in recent years,summarises the advantages and challenges of the above methods in the detection of aflatoxins in milk and dairy products,and provides an outlook on the future development of aflatoxins detection methods in milk and dairy products.The future development of aflatoxins detection methods in milk and dairy products is also discussed,which may provide some new ideas for the detection of aflatoxins in milk and dairy products.

Key words: milk; dairy products; aflatoxins; advances

中图分类号:

S859.84

张振宇, 王勇. 乳与乳制品中黄曲霉毒素检测研究进展[J]. 中国畜牧兽医, 2022, 49(7): 2820-2830.

ZHANG Zhenyu, WANG Yong. Advances on the Detection of Aflatoxins in Milk and Dairy Products[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2820-2830.

参考文献

[1] 张禹,王海荣.饲料中黄曲霉毒素的危害及脱毒方法进展[J].饲料研究,2021,8:157-160. ZHANG Y,WANG H R.The harm of aflatoxin in feed and the progress of detoxification methods[J].Feed Research,2021,8:157-160.(in Chinese)
[2] 魏姜勉.饲料中黄曲霉毒素检测及其对牛奶品质的影响[J].中国饲料,2021,22:69-73. WEI J M.Detection of aflatoxin in feed and its effect on milk quality[J].China Feed,2021,22:69-73.(in Chinese)
[3] 王文欣.微生物降解饲料中黄曲霉毒素的研究进展[J].饲料研究,2021,9:15-17. WANG W X.Research progress on microbial degradation of aflatoxin in feed[J].Feed Research,2021,9:15-17.(in Chinese)
[4] 刘配莲,张刚,陈焱,等.食品与饲料中黄曲霉毒素脱除技术的研究进展[J].中国油脂,2021,46(10):92-97. LIU P L,ZHANG G,CHEN Y,et al.Progress on detoxification technology of aflatoxin in food and feed[J].China Oils and Fats,2021,46(10):92-97.(in Chinese)
[5] 李瑞银,闫金玲,张喆萍,等.饲料中黄曲霉毒素B₁的致毒机理及其生物脱毒技术研究进展[J].饲料研究,2020,10:109-112. LI R Y,YAN J L,ZHANG Z P,et al.Research progress on toxic mechanism and biological detoxification technology of aflatoxin B₁ in feed[J].Feed Research,2020,10:109-112.(in Chinese)
[6] 刘明理,曹进,张庆生,等.牛奶及乳粉中黄曲霉毒素M₁的液相色谱-质谱检测比较研究[J].药物分析杂志,2017,37(3):471-476. LIU M L,CAO J,ZHANG Q S,et al.Comparative study on determination of aflatoxins M₁ in milk and its powder by liquid chromatography-mass spectrometry[J].Chinese Journal of Pharmaceutical Analysis,2017,37(3):471-476.(in Chinese)
[7] 郭露露.基于共价有机骨架TpBD构建乳制品中黄曲霉毒素M₁的电化学生物传感器[D].无锡:江南大学,2020. GUO L L.Construction of electrochemical biosensor for aflatoxin M₁ in dairy products based on covalent organic frameworks TpBD[D].Wuxi:Jiangnan University,2020.(in Chinese)
[8] PARKER C O,TOTHILL I E.Development of an electrochemical immunosensor for aflatoxin M₁ in milk with focus on matrix interference[J].Biosensors and Bioelectronics,2009,24:2452-2457.
[9] JAISWAL P,JHA S N,KAUR J,et al.Detection of aflatoxin M₁ in milk using spectroscopy and multivariate analyses[J].Food Chemistry,2018,238:209-214.
[10] 周贻兵,林野,李磊,等.高效液相色谱法测定牛奶中黄曲霉毒素M₁的含量[J].食品研究与开发,2014,35(15):96-98. ZHOU Y B,LIN Y,LI L,et al.Determination of aflatoxin M₁ content in milk by high-performance liquid chromatography[J].Food Research and Development,2014,35(15):96-98.(in Chinese)
[11] HERZALLAH S M.Determination of aflatoxins in eggs,milk,meat and meat products using HPLC fluorescent and UV detectors[J].Food Chemistry,2009,114:1141-1146.
[12] 罗晓琴,赵正苗,吴继华,等.牛奶中黄曲霉毒素M₁胶体金试纸条的研究[J].食品研究与开发,2014,35(1):76-77. LUO X Q,ZHAO Z M,WU J H,et al.Study on gold immunochromatography assay for rapid detection of aflatoxin M₁ in milk[J].Food Research And Development,2014,35(1):76-77.(in Chinese)
[13] 宗万里,鲁刚.液相色谱-三重四级杆串联质谱法测定液态乳中黄曲霉毒素M₁含量[J].中国奶牛,2021,6:58-61. ZONG W L,LU G.Determination of aflatoxin M₁ in liquid milk by LC-MS/MS[J].China Dairy Cattle,2021,6:58-61.(in Chinese)
[14] ZHANG Y,HUANG Y X,YUE Z F,et al.Preparation and application of aptamer-functionalized sorbent for the analysis of ultra-trace aflatoxin M₁ and analogues in milk[J].Microchemical Journal,2021,166:106179.
[15] 华宇,高和杨,聂兴娜,等.同位素内标-高效液相色谱-串联质谱法检测牛奶及奶粉中黄曲霉毒素M₁[J].食品安全质量检测学报,2020,11(6):1978-1984. HUA Y,GAO H Y,NIE X N,et al.Determination of aflatoxin M₁ in milk and milk powder by isotope internal standard-high performance liquid chromatography-tandem mass spectrometry[J].Journal of Food Safety and Quality,2020,11(6):1978-1984.(in Chinese)
[16] DU L J,WANG S L,HUANG J P,et al.Determination of aflatoxin M₁ and B₁ in milk and jujube by miniaturized solid-phase extraction coupled with ultra high performance liquid chromatography and quadrupole time-of-flight tandem mass spectrometry[J].Journal of Separation Science,2018,41(19):3677-3685.
[17] MEHTA R,SHETTY S A,YOUNG M F,et al.Quantification of aflatoxin and ochratoxin contamination in animal milk using UHPLC-MS/SRM method:A small-scale study[J].Journal of Food Science and Technology,2021,58:3453-3464.
[18] 曹叶中,蔡文.基于HPLC-MS测定牛奶和奶粉中黄曲霉毒素方法的优化[J].安徽农业科学,2021,49(2):201-203. CAO Y Z,CAI W.Optimization of aflatoxin determination methods in milk and milk powder by HPLC-MS[J].Journal of Anhui Agricultural Science,2021,49(2):201-203.(in Chinese)
[19] 张俊,赵玉玺,张爱凤,等.液相色谱-质谱/质谱法检测生鲜乳中黄曲霉素M₁方法的建立[J].黑龙江畜牧兽医,2018,7:226-228. ZHANG J,ZHAO Y X,ZHANG A F,et al.Development of a liquid chromatography-mass spectrometry/mass spectrometry method for the determination of aflatoxin M₁ in raw milk[J].Heilongjiang Animal Science and Veterinary Medicine,2018,7:226-228.(in Chinese)
[20] PÉREZ E,MARTÍNEZ-PEINADO P,MARCO F,et al.Determination of aflatoxin M₁ in milk samples by means of an inductively coupled plasma mass spectrometry-based immunoassay[J].Food Chemistry,2017,230:721-727.
[21] 张莉蕴,王延新,王玉可,等.电化学生物传感器在真菌毒素检测中的研究进展[J].中国畜牧兽医,2019,46(9):2769-2777. ZHANG L Y,WANG Y X,WANG Y K,et al.Research progress on electrochemical biosensors in detection of mycotoxins[J].Chinese Animal Husbandry & Veterinary Medicine,2019,46(9):2769-2777.(in Chinese)
[22] 惠媛媛,王毕妮,张富新,等.基于还原氧化石墨烯的电化学适配体传感器对黄曲霉毒素M₁的检测[J].食品工业科技,2021,42(14):249-256. HUI Y Y,WANG B N,ZHANG F X,et al.An electrochemical aptasensor for detection of aflatoxin M₁ based on reduced graphene oxide[J].Science and Technology of Food Industry,2021,42(14):249-256.(in Chinese)
[23] HUANG Q T,LIN X F,CHEN D J,et al.Carbon dots/α-Fe₂O₃-Fe₃O₄nanocomposite:Efficient synthesis and application as a novel electrochemical aptasensor for the ultrasensitive determination of aflatoxin B₁[J].Food Chemistry,2022,373:131415.
[24] AHMADI S F,HOJJATOLESLAMY M,KIANI H,et al.Monitoring of aflatoxin M₁ in milk using a novel electrochemical aptasensor based on reduced graphene oxide and gold nanoparticles[J].Food Chemistry,2022,373:131321.
[25] SMOLKO V,SHURPIK D,PORFIREVA A,et al.Electrochemical aptasensor based on poly (neutral red) and carboxylated pillar
[5] arene for sensitive determination of aflatoxin M₁[J].Electroanalysis,2018,30:1-12.
[26] SHADJOU R,HASANZADEH M,HEIDAR-POOR M,et al.Electrochemical monitoring of aflatoxin M₁ in milk samples using silver nanoparticles dispersed on α-cyclodextrin-GQDs nanocomposite[J].Journal of Molecular Recognition,2018,31(6):e2699.
[27] HAMAMI M,MARS A,RAOUAFI N.Biosensor based on antifouling PEG/gold nanoparticles composite for sensitive detection of aflatoxin M₁ in milk[J].Microchemical Journal,2021,165:106102.
[28] RAHMANI H R,ADABI M,BAGHERI K P,et al.Development of electrochemical aptasensor based on gold nanoparticles and electrospun carbon nanofibers for the detection of aflatoxin M₁ in milk[J].Journal of Food Measurement and Characterization,2021,15:1826-1833.
[29] ABERA B D,FALCO A,IBBA P,et al.Development of flexible dispense-printed electrochemical immunosensor for aflatoxin M₁ detection in milk[J].Sensors,2019,19:3912.
[30] AISSA S B,MARS A,CATANANTE G,et al.Design of a redox-active surface for ultrasensitive redox capacitive aptasensing of aflatoxin M₁ in milk[J].Talanta,2019,195:525-532.
[31] KARCZMARCZYK A,BAEUMNER A J,FELLER K H.Rapid and sensitive inhibition-based assay for the electrochemical detection of ochratoxin A and aflatoxin M₁ in red wine and milk[J].Electrochimica Acta,2017,243:82-89.
[32] 杜树新,蒋丹红.基于STM的三维荧光导数光谱法检测食品中黄曲霉素[J].光电子·激光,2014,25(3):545-550. DU S X,JIANG D H.Utilizing 3-D first-order derivative fluorescence spectrometry to detect aflatoxin in foods based on STM[J].Journal of OptoelectronicsLaser,2014,25(3):545-550.(in Chinese)
[33] 张仁梅.植物油中黄曲霉毒素的红外检测法及琼脂糖光学特性研究[D].广州:暨南大学,2016. ZHANG R M.Research on infrared detection of aflatoxin in vegetable oil and optical characteristics of agarose gel[D].Guangzhou:Jinan University,2016.(in Chinese)
[34] LI G M,LIU C,ZHANG X C,et al.Highly photoluminescent carbon dots-based immunosensors for ultrasensitive detection of aflatoxin M₁ residues in milk[J].Food Chemistry,2021,355:129443.
[35] 郭婷,林淑凤,马良,等.基于磁性纳米材料和适配体的荧光传感器检测牛奶中黄曲霉毒素M₁[J].食品与发酵工业,2019,45(5):218-223. GUO T,LIN S F,MA L,et al.A fluorescent biosensor based on magnetic nanoparticles and aptamer for detecting AFM₁ in milk[J].Food and Fermentation Industries,2019,45(5):218-223.(in Chinese)
[36] LI H,YANG D B,LI P W,et al.Palladium nanoparticles-based fluorescence resonance energy transfer aptasensor for highly sensitive detection of aflatoxin M₁ in milk[J].Toxins,2017,9:318.
[37] JIA Y M,WU F,LIU P L,et al.A label-free fluorescent aptasensor for the detection of Aflatoxin B₁ in food samples using AIEgens and graphene oxide[J].Talanta,2019,198:71-77.
[38] GUO X D,WEN F,QIAO Q Q,et al.A novel graphene oxide-based aptasensor for amplified fluorescent detection of aflatoxin M1 in milk powder[J].Sensors,2019,19:3840.
[39] NIAZI S,KHAN I M,YU Y,et al.A novel fluorescent aptasensor for aflatoxin M₁ detection using rolling circle amplification and g-C₃N₄ as fluorescence quencher[J].Sensors and Actuators B:Chemical,2020,315:128049.
[40] ZHOU Y F,XIONG S C,ZHANG K K,et al.Quantum bead-based fluorescence-linked immunosorbent assay for ultrasensitive detection of aflatoxin M₁ in pasteurized milk,yogurt,and milk powder[J]. Journal of Dairy Science,2019,102(5):3985-3993.
[41] QIAO Q Q,GUO X D,WEN F,et al.Aptamer-based fluorescence quenching approach for detection of aflatoxinM₁ in milk[J].Frontiers in Chemistry,2021,9:653869.
[42] JHA A N,JAISWAL P,KAUR J,et al.Rapid detection and quantification of aflatoxin B₁ in milk using fourier transform infrared spectroscopy[J]. Journal of The Institution of Engineers (India):Series A,2021,102:259-265.
[43] JAISWAL P,JHA S N,KAUR J,et al.Detection of aflatoxin M₁ in milk using spectroscopy and multivariate analyses[J]. Food Chemistry,2018,238:209-214.
[44] LERDSRI J,SOONGSONG J,LAOLUE P,et al.Reliable colorimetric aptasensor exploiting 72-Mers ssDNA and gold nanoprobes for highly sensitive detection of aflatoxin M₁ in milk[J].Journal of Food Composition and Analysis,2021,102:103992.
[45] FANG B L,XU S L,HUANG Y J,et al.Gold nanorods etching-based plasmonic immunoassay for qualitative and quantitative detection of aflatoxin M₁ in milk[J].Food Chemistry,2020,329:127160.
[46] JALALIAN S H,LAVAEE P,RAMEZANI M,et al.An optical aptasensor for aflatoxin M₁ detection based on target-induced protection of gold nanoparticles against salt-induced aggregation and silica nanoparticles[J].SpectrochimicaActa Part A:Molecular and Biomolecular Spectroscopy,2021,246:119062.
[47] TSOUNIDI D,KOUKOUVINOS G,PETROU P,et al.Rapid and sensitive label-free determination of aflatoxin M₁ levels in milk through a white light reflectance spectroscopyimmunosensor[J].Sensors and Actuators B:Chemical,2019,282:104-111.
[48] 陈瑞春,段文仲,吕红英,等.免疫亲和层析净化-高效液相色谱法同时测定牛奶和奶粉中黄曲霉毒素B₁、B₂、G₁、G₂、M₁、M₂[J].食品科技,2011,36(8):300-304. CHEN R C,DUAN W Z,LYU H Y,et al.Determination of aflatoxin B₁,B₂,G₁,G₂,M₁,M₂ in milk and milk powder by immunoaffinity chromatography and high-performance liquid chromatography method[J].Food Science and Technology,2011,36(8):300-304.(in Chinese)
[49] 周贻兵,林野,李磊,等.高效液相色谱法测定牛奶中黄曲霉毒素M₁的含量[J].食品研究与开发,2014,35(15):95-98. ZHOU Y B,LIN Y,LI L,et al.Determination of aflatoxin M₁ content in milk by high-performance liquid chromatography[J].Food Research and Development,2014,35(15):95-98.(in Chinese)
[50] HAMED A M,ABDEL-HAMID M,GÁMIZ-GRACIA L,et al.Determination of aflatoxins in plant-based milk and dairy products by dispersive liquid-liquid microextraction and high-performance liquid chromatography with fluorescence detection[J].Analytical Letters,2019,52(2):363-372.
[51] SHUIB N S,MAKAHLEH A,SALHIMI S M,et al.Determination of aflatoxin M₁ in milk and dairy products using high performance liquid chromatography-fluorescence with post column photochemical derivatization[J].Journal of Chromatography A,2017,1510:51-56.
[52] SHUIB N S,SAAD B.In-syringe dispersive micro-solid phase extraction method for the HPLC-fluorescence determination of aflatoxins in milk[J].Food Control,2022,132:108510.
[53] SU Z X,ZHAO G Y,DOU W C.Determination of trace aflatoxin M₁(AFM₁) residue in milk by an immunochromatographic assay based on (PEI/PSS)₄ red silica nanoparticles[J].MicrochimicaActa,2020,187:658.
[54] HAN M M,GONG L,WANG J Y,et al.An octuplex lateral flow immunoassay for rapid detection of antibiotic residues,aflatoxin M₁ and melamine in milk[J].Sensors and Actuators B:Chemical,2019,292:94-104.
[55] LI M,WANG H M,SUN J D,et al.Rapid,on-site,and sensitive detection of aflatoxin M₁ in milk products by using time-resolved fluorescence microsphere test strip[J].Food Control,2021,121:107616.
[56] 蔡芬,陈伟.侧向层析试纸条现场检测牛奶中黄曲霉毒素M₁[J].合肥工业大学学报(自然科学版),2021,44(6):845-850. CAI F,CHEN W.On-site detection of aflatoxin M₁ in milk based on lateral flow test strips[J].Journal of Hefei University of Technology (Natural Science),2021,44(6):845-850.(in Chinese)
[57] KASOJU A,SHAHDEO D,KHAN A A,et al.Fabrication of microfluidic device for aflatoxin M₁ detection in milk samples with specific aptamers[J].Scientific Reports,2020,10:4627.
[58] 龚频,王思远,陈雪峰,等.胶体金免疫层析试纸条技术及其在食品安全检测中的应用研究进展[J].食品工业科技,2019,40(13):358-364. GONG P,WANG S Y,CHEN X F,et al.Research progress of colloidal gold immunochromatographic test strip technology and its application in food safety testing[J].Science and Technology of Food Industry,2019,40(13):358-364.(in Chinese)
[59] GIOVANNI S D,ZAMBRINI V,VARRIALE A,et al.Sweet sensor for the detection of aflatoxin M₁ in whole milk[J].ACS Omega,2019,4:12803-12807.
[60] YAO Y Y,WANG H X,WANG X Z,et al.Development of a chemiluminescentaptasensor for ultrasensitive and selective detection of aflatoxin B₁ in peanut and milk[J].Talanta,2019,201:52-57.