热应激对海南黑山羊精液抗氧化能力及精浆代谢组学特征的影响

doi:10.16431/j.cnki.1671-7236.2025.03.021

摘要/Abstract

摘要： 【目的】研究海南黑山羊精液在热应激状态下抗氧化能力的改变，并筛选精浆中可能存在的生物标志物。【方法】采集海南黑山羊精液，模拟体外热应激处理，将精液样品随机分为对照组(Con)和热应激组(HS)，测定精液抗氧化指标；利用液相色谱-质谱联用法对精浆进行非靶向代谢组分析，筛选差异代谢物，并进行KEGG通路富集分析。【结果】与对照组相比，热应激组海南黑山羊精液中总抗氧化能力(T-AOC)和谷胱甘肽过氧化物酶(GSH-Px)活性显著或极显著降低(P＜0.05；P＜0.01)，活性氧(ROS)和丙二醛(MDA)含量均显著或极显著升高(P＜0.05；P＜0.01)；线粒体膜电位(MMP)和三磷酸腺苷(ATP)浓度均显著降低(P＜0.05)。正交偏最小二乘法-判别分析(OPLS-DA)结果显示，热应激组海南黑山羊精浆代谢发生明显变化；聚类分析结果显示，组间差异显著且具有明显聚类。共筛选到64种差异代谢物，其中精浆中2-脱氧核糖5-磷酸、丙二酸、D-精氨酸、甲基多巴酸和烟酰胺单核苷酸可能通过嘧啶代谢、β-丙氨酸代谢、D-氨基酸代谢、烟酸和烟酰胺等代谢途径响应热应激。【结论】热应激显著降低了海南黑山羊精液品质及抗氧化能力，精浆中丙二酸、D-精氨酸、甲基多巴酸和烟酰胺单核苷酸可作为海南黑山羊精液热应激的潜在生物标志物。

关键词: 海南黑山羊; 热应激; 精液; 代谢组学; 生物标志物

Abstract: 【Objective】 The purpose of this experiment was to investigate the alterations in the antioxidant capacity of semen in Hainan Black goats under heat stress,and screen the potential biomarkers in seminal plasma.【Method】 Simulated in vitro heat stress treatment,the semen of Hainan Black goats was collected,and the samples were randomly divided into control group (Con) and heat stress group (HS),and the antioxidant indices of semen were determined.Additionally,non-targeted metabolome analysis of the seminal plasma was conducted using liquid chromatography-mass spectrometry,the differential metabolites were screened,and KEGG pathway enrichment analysis was carried out.【Result】 Compared with control group,the total antioxidant capacity (T-AOC) and the activity of glutathione peroxidase (GSH-Px) in semen of Hainan Black goats in heat stress group was significantly or extremely significantly decreased (P＜0.05 or P＜0.01),and the contents of reactive oxygen species (ROS) and malondialdehyde (MDA) were significantly or extremely significantly increased (P＜0.05 or P＜0.01),mitochondrial membrane potential (MMP) and adenosine triphosphate (ATP) concentration were significantly decreased (P＜0.05).The results of orthogonal partial least square discriminant analysis (OPLS-DA) indicated that there were significant variations in seminal plasma metabolism of Hainan Black goats in heat stress group,and the cluster analysis demonstrated that there was significant differences and distinct clustering between the two groups.A total of 64 distinct metabolites were identified.Among them,2-deoxyribose 5-phosphate,malonic acid,D-arginine,methyldopa and niacinamide mononucleotides in seminal plasma might respond to heat stress via pyrimidine metabolism,β-alanine metabolism,D-amino acid metabolism,niacin and niacinamide metabolism.【Conclusion】 Heat stress was significantly decreased the semen quality and antioxidant capacity of Hainan Black goats.Malonic acid,D-arginine,methyldopa acid and niacinamide mononucleotides in seminal plasma were served as potential biomarkers of the heat stress state in semen of Hainan Black goats.

Key words: Hainan Black goats; heat stress; semen; metabolomics; biomarker

中图分类号:

S827.3

安兆翔, 荀文娟, 周汉林, 施力光. 热应激对海南黑山羊精液抗氧化能力及精浆代谢组学特征的影响[J]. 中国畜牧兽医, 2025, 52(3): 1191-1201.

AN Zhaoxiang, XUN Wenjuan, ZHOU Hanlin, SHI Liguang. Effects of Heat Stress on Semen Antioxidant Capacity and Seminal Plasma Metabolomics in Hainan Black Goats[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1191-1201.

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