柯乐猪KRT10基因多态性及其与繁殖性状的关联分析

doi:10.16431/j.cnki.1671-7236.2024.06.025

摘要/Abstract

摘要： 【目的】研究角蛋白10(keratin 10,KRT10)基因多态性对柯乐猪繁殖性状的影响,以提高柯乐猪的繁殖力。【方法】以255头柯乐猪为研究对象,采用PCR产物测序、DANMAN序列比对软件及人工校对相结合的方法鉴定KRT10基因单核苷酸多态性(single nucleotide polymorphism,SNP)位点,利用SHEsis在线软件分析SNP位点的群体遗传特性,通过RNAfold在线工具对SNP位点进行生物信息学分析,使用SPSS 22.0软件中的一般线性模型分析KRT10基因SNP位点与柯乐猪繁殖性状的关联性。【结果】在柯乐猪KRT10基因中共鉴定到3个SNPs位点:第4内含子的g.21643703 C＞T和g.21643714 G＞A;第5外显子的g.21643741 G＞A。g.21643741 G＞A突变导致密码子由AAG突变为AAA,编码氨基酸均为赖氨酸(K),为同义突变,引起编码的mRNA二级结构发生改变。3个SNPs位点均检测到3种基因型,g.21643703 C＞T和g.21643741 G＞A属于中度多态位点(0.25＜PIC＜0.5),g.21643714 G＞A属于低度多态位点(PIC＜0.25)。g.21643703 C＞T和g.21643741 G＞A位点均处于Hardy-Weinberg平衡状态(P＞0.05),g.21643714 G＞A位点显著偏离Hardy-Weinberg平衡状态(P＜0.05);3个SNPs位点间均不存在强连锁不平衡。KRT10基因3个SNPs位点共产生4种单倍型和10种双倍型。关联分析结果表明,g.21643703 C＞T位点对柯乐猪初生窝重、断奶仔数和断奶窝重的影响均达到显著水平(P＜0.05);g.21643714 G＞A位点对柯乐猪总产仔数和断奶窝重的影响均达到显著水平(P＜0.05);g.21643741 G＞A位点对柯乐猪总产仔数、产活仔数、断奶仔数和断奶窝重的影响均达到显著水平(P＜0.05)。双倍型H3H3对柯乐猪总产仔数、产活仔数、断奶仔数和断奶窝重的影响最明显。【结论】KRT10基因中存在的3个SNPs位点对柯乐猪繁殖性状有显著影响,其中H3H3为有利双倍型,可作为柯乐猪繁殖性状选择的遗传标记。

关键词: 柯乐猪; KRT10基因; 单核苷酸多态性(SNP); 繁殖性状

Abstract: 【Objective】 The aim of this study was to investigate the effect of the polymorphism of keratin 10 (KRT10) gene on reproductive traits in Kele pigs,in order to improve the fertility of Kele pigs.【Method】 There were 255 Kele pigs used in this experiment.The single nucleotide polymorphism (SNP) of KRT10 gene was identified using a combination of PCR product sequencing,DANMAN sequence alignment software and manual verification.The population genetic characteristics of SNP were analyzed by SHEsis online software,and the bioinformatics analysis of SNP was conducted by RNAfold online tool.The general linear model of SPSS 22.0 software was used to analyze the correlation between SNP of KRT10 gene and reproductive traits in Kele pigs.【Result】 Three SNPs were identified in KRT10 gene of Kele pigs:g.21643703 C＞T and g.21643714 G＞A in intron 4,and g.21643741 G＞A in exon 5.g.21643741 G＞A mutation caused a codon mutation from AAG to AAA,encoding amino acids of lysine (K),which was a synonymous mutation and caused a change of the secondary structure in mRNA.Three genotypes were detected at all three mutation sites.g.21643703 C＞T and g.21643741 G＞A belonged to moderate polymorphism sites (0.25＜PIC＜0.5),and g.21643714 G＞A belonged to low polymorphism site (PIC＜0.25).g.21643703 C＞T and g.21643741 G＞A were in Hardy-Weinberg equilibrium (P＞0.05),g.21643714 G＞A was significantly deviated from Hardy-Weinberg equilibrium (P＜0.05).There were no strong linkage imbalance among three SNPs.Three SNPs produced a total of 4 haplotypes and 10 diploids.The results of correlation analysis showed that the effect of g.21643703 C＞T on newborn litter weight,weaned piglets and weaned litter weight in Kele pigs reached a significant level (P＜0.05),the effect of g.21643714 G＞A on total litter size and weaned litter weight in Kele pigs reached a significant level (P＜0.05),and the effect of g.21643741 G＞A on total litter size,live litter size,weaned piglets and weaned litter weight in Kele pigs reached a significant level (P＜0.05).The diploid H3H3 had the most significant effects on total litter size,live litter size,weaned piglets and weaned litter weight in Kele pigs.【Conclusion】 Three SNPs of KRT10 gene had significant effects on reproductive traits in Kele pigs,and H3H3 was advantageous diplotype,which might be used as a genetic marker for reproductive trait selection in Kele pigs.

Key words: Kele pigs; KRT10 gene; single nucleotide polymorphism (SNP); reproductive traits

中图分类号:

S813.3

赵永, 杨齐心, 李维, 熊力, 杨红文, 王春源, 吴燕, 向进, 张依裕. 柯乐猪KRT10基因多态性及其与繁殖性状的关联分析[J]. 中国畜牧兽医, 2024, 51(6): 2508-2516.

ZHAO Yong, YING Qixin, LI Wei, XIONG Li, YANG Hongwen, WANG Chunyuan, WU Yan, XIANG Jin, ZHANG Yiyu. Polymorphisms of KRT10 Gene and Its Association Analysis with Reproductive Traits in Kele Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2508-2516.

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