云上黑山羊28号染色体上3个SNPs位点多态性与产羔数性状的关联分析

doi:10.16431/j.cnki.1671-7236.2021.04.016

摘要/Abstract

摘要： 为验证和挖掘山羊产羔性状的分子标记和主效基因，选取前期研究获得的位于云上黑山羊28号染色体上3个与产羔数性状存在潜在关联的SNPs位点（rs268286450、rs268286391和rs268244272），采用MassARRAY飞行质谱列阵技术对541个云上黑山羊DNA样本进行SNP分型，并结合其产羔数进行关联分析。结果显示，3个SNPs位点均存在多态性，rs268286450位点在28号染色体第28 498 787 bp处发生C→T碱基突变，rs268286391位点在28号染色体第30 971 277 bp处发生A→C碱基突变，rs268244272位点在28号染色体第2 707 491 bp处发生T→C碱基突变，3个位点多态信息含量（PIC）分别为0.36、0.32和0.21；rs268286450和rs268286391位点处于Hardy-Weinberg不平衡状态（P<0.01），而rs268244272位点处于Hardy-Weinberg平衡状态（P>0.05）；rs268244272位点存在3种基因型CC、TT和TC，TT基因型频率为0.75，为野生基因型，TC基因型频率为0.22，为突变基因型，3种基因型最小二乘均值比较显示，TC基因型产羔数最小二乘均值与TT基因型差异极显著（P<0.01），与CC基因型差异不显著（P>0.05），TT与CC基因型产羔数最小二乘均值差异也不显著（P>0.05），TC基因型云上黑山羊产羔数上调。关联分析结果显示，rs268286450和rs268286391位点多态性与云上黑山羊产羔数关联不显著（P>0.05），rs268244272位点多态性与产羔数性状存在显著相关（P<0.05）。综上，云上黑山羊28号染色体上rs268244272位点多态性与产羔数性状显著相关，可作为云上黑山羊产羔数性状的分子标记在其高繁品系选育中加以应用。

关键词: 云上黑山羊; 28号染色体; SNP位点; 飞行质谱; 产羔数; 关联分析

Abstract: In order to verify and discover molecular markers and major genes of lambing traits,541 samples' SNP genotyping of three SNPs (rs268286450,rs268286391 and rs268244272) on chromosome 28 which were selected from Yunshang Black goat in early research was performed by MassARRAY time flight mass spectrometry,and analyzed the relationship between polymorphism of three SNPs and the lambing number in Yunshang Black goat.The results showed that there were a C→T base mutation at 28 498 787 bp of rs268286450,a A→C base mutation at 30 971 277 bp of rs268286391 and a T→C base mutation at 2 707 491 of rs268244272 on chromosome 28.The polymorphism information content (PIC) of three SNPs were 0.36,0.32 and 0.21,respectively.rs268286450 and rs268286391 were in Hardy-Weinberg disequilibrium (P<0.01),while rs268244272 was in Hardy-Weinberg equilibrium (P>0.05).The locus rs268244272 had three genotypes (CC,TT and TC).The wild genotype was TT and its genotype frequency was 0.75.The genotype frequency of TC was 0.22,which was a mutant genotype.The difference of the least squares mean in lambing number between TC and TT genotypes was extremely significant (P<0.01),but not significantly different from CC genotype (P>0.05).There was no significant difference of the least squares mean in lambing number between TT and CC genotypes (P>0.05).The results of association analysis showed that there was no significant correlation between lambing number of Yunshang Black goat and the polymorphism of rs268286450 and rs268286391 (P>0.05),but a significant association existed between the polymorphism of rs268244272 and lambing number (P<0.05).In conclusion,rs268244272 polymorphism on chromosome 28 of Yunshang Black goat was significantly associated with lambing number,and might be used as a molecular marker of lambing number in breeding of high reproduction straits in Yunshang Black goat.

Key words: Yunshang Black goat; SNP locus; chromosome 28; flight mass spectrometry; lambing number; association analysis

中图分类号:

S813.3

朱兰, 王鹏, 洪琼花, 杨红远, 赵小红, 兰蓉. 云上黑山羊28号染色体上3个SNPs位点多态性与产羔数性状的关联分析[J]. 中国畜牧兽医, 2021, 48(4): 1294-1301.

ZHU Lan, WANG Peng, HONG Qionghua, YANG Hongyuan, ZHAO Xiaohong, LAN Rong. Association Analysis of Three SNPs on Chromosome 28 with Lambing Number in Yunshang Black Goat[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1294-1301.

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