两个呼伦贝尔羊品系不同部位脂肪细胞形态及脂肪代谢相关基因表达差异分析

doi:10.16431/j.cnki.1671-7236.2019.12.023

摘要/Abstract

摘要： 试验旨在探讨呼伦贝尔羊巴尔虎品系（半椭圆状尾）和短尾品系（小桃状尾）脂肪沉积差异的分子机制。随机选择处于相同饲养管理条件下5月龄呼伦贝尔母羊21只（其中巴尔虎品系11只、短尾品系10只），测量其体尺、尾型和胴体重指标。组织切片观察8个部位脂肪细胞的形态，测定脂肪组织甘油三酯（TG）含量，并进一步用实时荧光定量PCR检测脂肪代谢相关基因在不同脂肪组织中的表达差异。结果显示，同月龄两品系羊胴体重、体尺及尾型指标差异显著或极显著（P<0.05；P<0.01）。虽然两品系羊同一部位的脂肪细胞形态基本一致，但巴尔虎品系尾部脂肪细胞面积和体积均大于短尾品系（P<0.01），并且皮下、网膜和尾部脂肪组织中的TG含量在两品系羊间差异显著或极显著（P<0.05；P<0.01）。ATGL、PPARγ、C/EBPα和SREBP 4个脂代谢相关基因在巴尔虎品系个体中的mRNA水平均显著或极显著高于短尾品系（P<0.05；P<0.01）；巴尔虎品系中ATGL和SREBP基因的表达与TG含量分别呈显著负相关和正相关（r=-0.965、0.972）。综上所述，两个呼伦贝尔羊品系在生长和脂肪代谢等方面存在较大差异。本研究结果可为进一步深入解析绵羊脂尾形成的分子机制提供理论依据。

关键词: 呼伦贝尔羊; 脂肪组织; 脂肪代谢; 基因表达

Abstract: To investigate the fat deposition mechanism of Hulun Buir sheep,twenty one Hulun Buir ewes at five months old were divided into two groups,Baerhu group(semi-ellipsoid tail,n=11) and short-tailed group(small-peach tail,n=10),and fed under the same management conditions.The carcass weight,body size and tail type indexes of two groups were measured.Adipose tissues from 8 body locations were used to analyze adipocyte morphology and triglyceride(TG) content.Moreover,the adipose metabolism related genes were detected by Real-time quantitative PCR.The result showed that two populations were significantly or extremely significantly differed in length,width and weight of tail as well as carcass weight and body length (P<0.05;P<0.01).Although the morphology of adipocytes in the same part of two populations were basically the same,the area and volume of adipocytes in the tail of Baerhu population were larger than that of short-tailed ewes (P<0.01).And TG content in adipose tissues including subcutaneous,omentum and tail tissues were significantly or extremely significantly different between two sheep groups (P<0.05;P<0.01).The mRNA levels of lipid-metabolism related genes,including ATGL,PPARγ,C/EBPα and SREBP,were higher in Baerhu population than that in short-tailed group (P<0.05; P<0.01).For the Baerhu ewes,the expression of ATGL and SREBP was negatively or positively associated with the TG content (r=-0.965,0.972),respectively.In summary,it was preliminarily revealed that it had significant differences of adipose deposition ability between two populations.The study was helpful for further exploring the molecular mechanism of fat tail formation in sheep and providing a theoretical basis.

Key words: Hulun Buir sheep; adipose tissue; adipocyte metabolism; gene expression

中图分类号:

S813.9

安宗麒, 孙伟芳, 郭家中, 李利, 张红平. 两个呼伦贝尔羊品系不同部位脂肪细胞形态及脂肪代谢相关基因表达差异分析[J]. 中国畜牧兽医, 2019, 46(12): 3650-3658.

AN Zongqi, SUN Weifang, GUO Jiazhong, LI Li, ZHANG Hongping. Difference Analysis of Adipocyte Morphology and Adipogenic Gene Expression Between Two Hulun Buir Sheep Populations[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3650-3658.

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