FNDC5在小鼠老龄化过程脂肪组织中的表达变化

doi:10.16431/j.cnki.1671-7236.2020.10.011

中国畜牧兽医 ›› 2020, Vol. 47 ›› Issue (10): 3132-3139.doi: 10.16431/j.cnki.1671-7236.2020.10.011

FNDC5在小鼠老龄化过程脂肪组织中的表达变化

李艺雷¹, 范云琳², 李玫毅³, 马佩¹, 吉树森¹, 孙睿¹, 罗小毛¹, 王海东¹

1. 山西农业大学动物科技学院, 太谷 030801;
2. 中国奶业协会, 北京 100193;
3. 河南省郑州市动物卫生监督所, 郑州 450000

收稿日期:2020-07-01 出版日期:2020-10-20 发布日期:2020-10-17
通讯作者: 王海东 E-mail:13623541993@163.com
作者简介:李艺雷(1993-),女,河南郑州人,博士,研究方向:动物肌肉发育及脂肪代谢,E-mail:13526583021@163.com;范云琳(1977-),女,北京人,畜牧师,研究方向:动物肌肉发育及脂肪代谢,E-mail:1211711852@qq.com
基金资助:
山西省重点研发项目（201803D221022-2）；山西省"1331工程"重点创新团队（20171115）；山西省优秀人才科技创新项目（201805D211012）

Expression Changes of FNDC5 in Adipose Tissue During Aging Process in Mice

LI Yilei¹, FAN Yunlin², LI Meiyi³, MA Pei¹, JI Shusen¹, SUN Rui¹, LUO Xiaomao¹, WANG Haidong¹

1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China;
2. Dairy Association of China, Beijing 100193, China;
3. Zhengzhou Animal Health Supervision Institute, Zhengzhou 450000, China

Received:2020-07-01 Online:2020-10-20 Published:2020-10-17

摘要/Abstract

摘要： 本研究旨在探讨Ⅲ型纤连蛋白域包含蛋白5（type Ⅲ fibronectin domain contains protein 5，FNDC5）在小鼠老龄化过程中脂肪组织中的表达变化，以小鼠老龄化过程中不同发育阶段的健康小鼠为研究对象，通过HE染色、免疫组织化学染色、实时荧光定量PCR和Western blotting等技术研究小鼠老龄化过程中脂肪组织FNDC5的表达以及定位。结果发现，FNDC5在小鼠老龄化过程中各个发育时期的脂肪中均有不同程度的表达并呈现明显的差异性。HE染色发现，随着年龄的增长，脂肪细胞的大小呈现先逐渐增大后萎缩的趋势。免疫组织化学染色发现，FNDC5在幼年小鼠的脂肪组织中表达水平最高，在性成熟的脂肪组织中表达水平次之，老年的脂肪组织中的表达水平低于性成熟时期，体成熟的脂肪组织中表达水平最低。实时荧光定量PCR和Western blotting检测结果也与免疫组织化学染色趋势相符。以上结果表明，FNDC5基因可能在脂肪组织中发挥一定的作用，为研究脂肪代谢以及肥胖相关的疾病提供新思路。

关键词: 小鼠; 脂肪组织; FNDC5; 老龄化过程; 表达定位

Abstract: In order to investigate the expression changes of type Ⅲ fibronectin domain contains protein 5 (FNDC5) in adipose tissue of mice during aging process,healthy mice at different developmental stages were used as research objects.In this experiment,HE staining,immunohistochemical staining,Real-time PCR and Western blotting techniques were used to study the expression and localization of FNDC5 in adipose tissue during aging process.The results showed that FNDC5 was expressed to varying degrees in the fats of all developmental stages in the aging process of mice and showed significant differences.HE staining showed that with age,the size of fat cells gradually increased and then shrank.Immunohistochemical staining showed that the expression level of FNDC5 in adipose tissue of young mice was the highest,followed by that of sexual mature adipose tissue,and that of aged adipose tissue was lower than that of sexual maturation,and the expression level of FNDC5 was the lowest in mature adipose tissue of young mice.Subsequent Real-time PCR and Western blotting results were also consistent with immunohistochemical staining trends.The above results indicated that the FNDC5 gene might play a certain role in adipose tissue,which would provide new ideas for the study of fat metabolism and obesity-related diseases.

Key words: mice; adipose tissue; FNDC5; aging process; expression positioning

中图分类号:

Q786

李艺雷, 范云琳, 李玫毅, 马佩, 吉树森, 孙睿, 罗小毛, 王海东. FNDC5在小鼠老龄化过程脂肪组织中的表达变化[J]. 中国畜牧兽医, 2020, 47(10): 3132-3139.

LI Yilei, FAN Yunlin, LI Meiyi, MA Pei, JI Shusen, SUN Rui, LUO Xiaomao, WANG Haidong. Expression Changes of FNDC5 in Adipose Tissue During Aging Process in Mice[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3132-3139.

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FNDC5在小鼠老龄化过程脂肪组织中的表达变化

Expression Changes of FNDC5 in Adipose Tissue During Aging Process in Mice

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