鸡肌内脂肪沉积相关候选基因筛选

doi:10.16431/j.cnki.1671-7236.2020.06.022

摘要/Abstract

摘要： 本研究对课题组前期在京星黄鸡转录组研究中筛选到的与肌内脂肪（IMF）差异沉积相关的14个候选基因进行验证，检测其在中外两个鸡种群体中与胸肌IMF沉积的关联性。以98日龄慢速型地方鸡京星黄鸡和42日龄快速型白羽科宝肉鸡胸肌组织为素材，通过胸肌甘油三酯（TG）含量区分高低表型组，并检测候选基因在组间的基因表达差异。结果表明，在京星黄鸡胸肌TG高、低组间ATP结合盒亚家族B成员8（ABCB8）、脂联素（ADIPOQ）、第6号染色体开放阅读框65（BEND6）、CD74分子（CD74）、核糖基5-磷酸转移酶（FKTN）、组蛋白乙酰转移酶1（HAT1）、硫酸乙酰肝素-氨基葡萄糖3-磺基转移酶5（HS3ST5）、介体复合物亚基4（MED4）、肿瘤坏死因子超家族成员8（TNFSF8）和TNFAIP3相互作用蛋白1（TNIP1）共10个基因表达差异显著（P<0.05）；在科宝肉鸡TG高、低组间ADIPOQ、BEND6、FKTN、HAT1、HS3ST5、MED4和TNIP1共7个基因表达差异显著（P<0.05）；ADIPOQ、FKTN、HAT1、HS3ST5、MED4和TNIP1共6个基因在两个品种中差异表达趋势一致（P<0.05）。本研究提供了鸡IMF沉积相关新候选基因，为IMF分子调控机理研究和相关分子标记筛选研究奠定了良好的基础。

关键词: 鸡; 肌内脂肪; 脂肪代谢基因; 实时荧光定量PCR

Abstract: In this study,14 candidate genes related to the differential deposition of chicken intramuscular fat (IMF) screened by the research team in the transcriptome study of Jingxing-Huang chicken were performed for verification.To detect the association between the expression level of candidate genes and breast muscle IMF deposition in two breeds.The breast muscle of 98-day-old slow-growing local Jingxing-Huang chickens and 42-day-old fast-growing white feather Cobb broilers were used to distinguish the high and low phenotypic groups based on the content of triglycerides in the breast muscle.The results showed that there were significant differences in expression of 10 genes of ABCB8,ADIPOQ,BEND6,CD74,FKTN,HAT1,HS3ST5,MED4,TNFSF8 and TNIP1 between Jingxing-Huang chicken breast muscle TG high and low groups (P<0.05).There were significant differences in 7 genes of ADIPOQ,BEND6,FKTN,HAT1,HS3ST5,MED4 and TNIP1 between Cobb broiler breast muscle TG high and low groups (P<0.05).6 genes including ADIPOQ,FKTN,HAT1,HS3ST5,MED4 and TNIP1 were significantly expressed in both varieties (P<0.05).This study provided new candidate genes related to chicken IMF deposition,laying a good foundation for the study of IMF molecular regulation mechanism and related molecular marker screening research.

Key words: chicken; intramuscular fat; fat metabolism genes; Real-time quantitative PCR

中图分类号:

S831.2

李京徽, 邢思远, 王希彩, 李庆贺, 赵桂苹, 张永宏, 文杰, 刘冉冉. 鸡肌内脂肪沉积相关候选基因筛选[J]. 中国畜牧兽医, 2020, 47(6): 1828-1836.

LI Jinghui, XING Siyuan, WANG Xicai, LI Qinghe, ZHAO Guiping, ZHANG Yonghong, WEN Jie, LIU Ranran. Screening of Candidate Genes Related to Intramuscular Fat Deposition in Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(6): 1828-1836.

参考文献

[1] FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS.The State of Food Security and Nutrition in the World 2017[M].Rome Italy:FAO,2017.
[2] SPORER K R B,TEMPELMAN R J,ERNST C W,et al.Transcriptional profiling identifies differentially expressed genes in developing turkey skeletal muscle[J].BMC Genomics,2011,12(1):143.
[3] PURCHAS R W,BURNHAM D L,MORRIS S T.Effects of growth potential and gowth path on tenderness of beef longissimus muscle from bulls and steers[J].Journal of Animal Science,2002,80(12):3211-3221.
[4] CUI H,ZHENG M,ZHAO G,et al.Identification of differentially expressed genes and pathways for intramuscular fat metabolism between breast and thigh tissues of chickens[J].BMC Genomics,2018,19(1):55.
[5] 袁华根.鸡肉汤香味成分鉴定及日龄、性别和酮体部位对鸡肉风味的影响[D].南京:南京农业大学,2007. YUAN H G.Identification of chicken broth aroma component and the effects of age,gender and carcass part to flavor of chicken[D].Nanjing:Nanjing Agricultural University,2007.(in Chinese)
[6] LI X,FU X,YANG G,et al.Enhancing intramuscular fat development via targeting fibro-adipogenic progenitor cells in meat animals[J].Animal,2020,14(2):312-321.
[7] WARNER R D,GREENWOOD P L,PETHICK D W,et al.Genetic and environmental effects on meat quality[J].Meat Science,2010,86(1):171-183.
[8] RANRAN L,HONGYANG W,JIE L,et al.Uncovering the embryonic development-related proteome and metabolome signatures in breast muscle and intramuscular fat of fast-and slow-growing chickens[J].BMC Genomics,2017,18(1):816.
[9] CUI H X,LIU R R,ZHAO G P,et al.Identification of differentially expressed genes and pathways for intramuscular fat deposition inpectoralis majortissues of fast-and slow-growing chickens[J].BMC Genomics,2012,13(1):213.
[10] 沙尔山别克·阿不地力大,舒婷,吴旭升,等.拜城油鸡A-FABP、H-FABP和PPAR-γ基因mRNA表达的发育性变化及其与肌内脂肪含量的相关性研究[J].中国家禽,2019,14:5-11. SHAERSHANBIEKE A,SHU T,WU X S,et al.Developmental changes of A-FABP,H-FABP and PPAR-γ genes mRNA expression and its correlation with intramuscular fat content in Baicheng You chicken[J].China Poultry,2019,14:5-11.(in Chinese)
[11] LANGFELDER P,HORVATH S.WGCNA:An R package for weighted correlation network analysis[J].BMC Bioinformatics,2008,9(1):559.
[12] ZEREHDARAN S,VEREIJKEN A L J,VAN ARENDONK J A M,et al.Estimation of genetic parameters for fat deposition and carcass traits in broilers[J].Poultry Science,2004,83(4):521-525.
[13] 黄甜,严成,黄业传,等.不同饲喂方式猪肌内脂肪组成及含量分析[J].湖北农业科学,2014,22:137-141. HUANG T,YAN C,HUANG Y C,et al.Intramuscular lipid content and fatty acid composition of pigs in different feeding modes[J].Hubei Agricultural Sciences,2014,22:137-141.(in Chinese)
[14] 经荣斌.中国地方品种猪肌肉脂肪酸含量的研究[J].猪业科学,2012,29(11):126-127. JING R B.Study on fatty acid content of Chinese native pig muscle[J].Swine Industry Science,2012,29(11):126-127.(in Chinese)
[15] 黄业传,李洪军,吴照民,等.不同部位荣昌猪肉中脂肪含量和脂肪酸组成对比[J].食品科学,2011,22:224-228. HUANG Y C,LI H J,WU Z M,et al.Comparison of muscle lipid content and fatty acid composition among different positions of Rongchang pork carcass[J].Food Science,2011,22:224-228.(in Chinese)
[16] LIANG P.ADIPOQ is a novel adipose-specific gene dysregulated in obesity[J].Journal of Biological Chemistry,1996,271(18):10697-10703.
[17] NADERALI E K,FATANI S,TELLES M,et al.The effects of physiological and pharmacological weight loss on adiponectin and leptin mRNA levels in the rat epididymal adipose tissue[J].European Journal of Pharmacology,2008,579(1-3):433-438.
[18] NAIR R P,DUFFIN K C,HELMS C,et al.Genome-wide scan reveals association of psoriasis with IL-23 and NF-κB pathways[J].Nature Genetics,2009,41(2):199.
[19] SCHULZE M S E D,WUCHERPFENNIG K W.The mechanism of HLA-DM induced peptide exchange in the MHC class Ⅱ antigen presentation pathway[J].Current Opinion in Immunology,2012,24(1):105-111.
[20] WANG C,LIN G H,MCPHERSON A J,et al.Immune regulation by 4-1BB and 4-1BBL:Complexities and challenges[J].Immunological Reviews,2009,229(1):192-215.
[21] CRAIG A S,GRUSS H J,TERRI D,et al.CD30 antigen,a marker for Hodgkin's lymphoma,is a receptor whose ligand defines an emerging family of cytokines with homology to TNF[J].Cell,1993,73(7):1349-1360.
[22] MOCHIZUKI H,YOSHIDA K,GOTOH M,et al.Characterization of a heparan sulfate 3-O-Sulfotransferase-5,an enzyme synthesizing a tetrasulfated disaccharide[J].Journal of Biological Chemistry,2003,278(29):26780-26787.
[23] WITOELAR A,RONGVE A,ALMDAHL I S,et al.Meta-analysis of Alzheimer's disease on 9,751 samples from Norway and IGAP study identifies four risk loci[J].Scientific Reports,2018,8(1):18088.
[24] TELLIS C C,TSELEPIS A D.The role of lipoprotein-associated phospholipase A2 in atherosclerosis may depend on its lipoprotein carrier in plasma[J].Biochimica et Biophysica Acta,2009,1791(5):327-338.
[25] ZHANG W,YANG B,ZHANG J,et al.Genome-wide association studies for fatty acid metabolic traits in five divergent pig populations[J].Scientific Reports,2016,6:24718.
[26] ZHANG Q H,YE M,WU X Y,et al.Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34⁺ hematopoietic stem/progenitor cells[J].Genome Research,2000,10(10):1546-1560.
[27] BENSON L J,PHILLIPS J A,GU Y,et al.Properties of the type B histone acetyltransferase Hat1:H4 tail interaction,site preference,and involvement in DNA repair[J].Journal of Biological Chemistry,2007,282(2):836-842.
[28] HAYASHI Y K,OGAWA M,TAGAWA K,et al.Selective deficiency of alpha-dystroglycan in Fukuyama-type congenital muscular dystrophy[J].Neurology,2001,57(1):115.
[29] PERCIVAL J M,FROEHNER S C.Golgi complex organization in skeletal muscle:A role for golgi-mediated glycosylation in muscular dystrophies?[J].Traffic,2007,8(3):184-194.