副乳头对奶牛生产性能的影响及其相关基因LGR5的遗传表达分析

doi:10.16431/j.cnki.1671-7236.2019.12.022

摘要/Abstract

摘要： 试验旨在探究副乳头（SNT）对奶牛产奶性能的影响及乳腺发育相关基因LGR5与副乳头发生的关系。收集325头有副乳头奶牛及同年出生的737头无副乳头奶牛的产奶性能数据，比较有、无副乳头奶牛间的产奶性能差异；利用实时荧光定量PCR技术检测有、无副乳头奶牛LGR5基因的表达差异，通过直接测序法测定有、无副乳头奶牛的LGR5基因序列，寻找该基因上的遗传突变。结果表明，无副乳头奶牛的日产奶量、高峰奶量及泌乳持续力等产奶性能均优于有副乳头奶牛，但未达到统计学显著水平；LGR5基因在有副乳头奶牛中的表达量显著高于无副乳头奶牛（P<0.05）；在LGR5基因第1内含子中检测到3个SNPs：rs42849472、rs42849471和rs42849470，有副乳头奶牛中3个SNPs的AA基因型频率均极显著高于无副乳头奶牛（P<0.01）。结果提示，LGR5基因可能与奶牛副乳头发生有关，可作为有效的候选基因。

关键词: 奶牛; 有副乳头; 无副乳头; 产奶性能; LGR5基因

Abstract: This study was aimed to investigate the effect of supernumerary teat (SNT) on the milk performance of dairy cows and the relationship between mammogenesis gene LGR5 and supernumerary teat.Dairy herd improvement (DHI) data of 325 supernumerary teat and 737 non-supernumerary teat dairy cows in the same born year were collected to compare the differences in milk performance.The differential expression of LGR5 gene between supernumerary teat and non-supernumerary teat dairy cows was performed by Real-time quantitative PCR.The genetic mutation of LGR5 gene was detected by direct sequencing method.The results showed that the daily milk yield,peak milk yield and lactation persistence of non-supernumerary teat dairy cows were better than that of supernumerary teat dairy cows,but did not reach the statistical significant level.The expression of LGR5 gene in supernumerary teat dairy cows was significantly higher than that in non-supernumerary teat dairy cows (P<0.05).3 SNPs (rs42849472,rs42849471 and rs42849470) were identified in LGR5 gene intron 1.The frequency of AA genotype of 3 SNPs in supernumerary teat dairy cows were extremely significantly higher than that in non-supernumerary teat dairy cows (P<0.01).The results suggested that LGR5 gene might be involved in the occurrence of supernumerary teat in dairy cows,and could be used as an effective candidate gene.

Key words: dairy cows; supernumerary teat; non-supernumerary teat; milk performance; LGR5 gene

中图分类号:

S813.3

文惠, 罗汉鹏, 米思远, 刘雪琴, 王雅春, 肖炜, 俞英. 副乳头对奶牛生产性能的影响及其相关基因LGR5的遗传表达分析[J]. 中国畜牧兽医, 2019, 46(12): 3642-3649.

WEN Hui, LUO Hanpeng, MI Siyuan, LIU Xueqin, WANG Yachun, XIAO Wei, YU Ying. Effect of Supernumerary Teat on Milk Performance and Genetic Expression Analysis of LGR5 Gene in Dairy Cows[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3642-3649.

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