小檗碱对黄羽肉鸡器官指数、抗氧化能力和肠道免疫功能的影响

doi:10.16431/j.cnki.1671-7236.2022.03.015

摘要/Abstract

摘要： 【目的】本试验旨在研究小檗碱对黄羽肉鸡器官指数、抗氧化能力和肠道免疫功能的影响。【方法】选用1日龄快大型岭南黄羽肉鸡360只，随机分为3组，每组6个重复，每重复20只。对照组（NC）饲喂基础饲粮；抗生素组（PC）饲喂基础饲粮+200 mg/kg土霉素钙+250 mg/kg那西肽；小檗碱组（BBR）饲喂基础饲粮+250 mg/kg小檗碱。在试验第21、42和63天，从每个重复中随机挑选1只接近平均体重的鸡，采集心脏、肝脏、脾脏和法氏囊分别称重，记录重量，计算其器官指数；刮取空肠黏膜，测定超氧化物歧化酶（T-SOD）、总抗氧化能力（T-AOC）、谷胱甘肽过氧化物酶（GSH-Px）活性和丙二醛（MDA）含量；提取空肠黏膜总RNA，合成cDNA，测定紧密连接蛋白与炎症因子相关基因的mRNA表达量。【结果】小檗碱对黄羽肉鸡器官指数无显著影响（P>0.05）。与对照组相比，在饲粮中添加小檗碱显著提高了63日龄黄羽肉鸡空肠黏膜的总抗氧化能力（T-AOC），显著降低了63日龄黄羽肉鸡空肠黏膜的丙二醛（MDA）含量（P<0.05）。抗生素组21日龄黄羽肉鸡空肠黏膜的闭合蛋白（Occludin）和闭锁小带蛋白-1（ZO-1）的mRNA表达量均显著高于对照组和小檗碱组（P<0.05）。与对照组和抗生素组相比，小檗碱显著提高了63日龄黄羽肉鸡空肠黏膜的跨膜蛋白-1（Claudin-1）的mRNA表达量（P<0.05），显著降低了21、42与63日龄黄羽肉鸡空肠黏膜的核苷酸结合寡聚化结构域蛋白1（NOD1）的mRNA表达量（P<0.05）；小檗碱组与抗生素组42日龄黄羽肉鸡空肠黏膜的白介素-1β（IL-1β）和白介素-8（IL-8）的mRNA表达量均显著低于对照组（P<0.05）；与对照组相比，小檗碱显著降低了63日龄黄羽肉鸡空肠黏膜的肿瘤坏死因子-α（TNF-α）、IL-1β、IL-8的mRNA表达量（P<0.05）。【结论】饲粮中添加250 mg/kg小檗碱可增强63日龄黄羽肉鸡肠道抗氧化能力，改善肠道屏障，降低肠道炎性因子的表达，从而改善肠道免疫功能。

关键词: 小檗碱; 黄羽肉鸡; 肠道屏障; 基因表达; 抗氧化; 肠道免疫

Abstract: 【Objective】 This experiment was conducted to investigate the effects of dietary berberine (BBR) on organ indexes, intestinal antioxidant capacity and intestinal immunity in Yellow-feathered broilers.【Method】 A total of 360 one-day-old Yellow-feathered chickens were randomly divided into 3 groups with 6 replicates in each group and 20 chickens in each replicate.The control group (NC) was fed with basal diet, the antibiotic group (PC) was fed with basal diet +200 mg/kg oxytetracycline calcium +250 mg/kg nasitide, the berberine group (BBR) was fed basal diet +250 mg/kg BBR.At 21, 42 and 63 d, one chick with the similar average body weight from each replicate was randomly selected and sacrificed.The heart, liver, spleen and bursa of Fabricius were collected and weighed respectively.The weight was recorded and the organ index was calculated.The jejunal mucosa was scraped to determine the activities of superoxide dismutase (T-SOD), total antioxidant capacity (T-AOC), and glutathione peroxidase (GSH-Px), as well as the maldialaldehyde (MDA) content.The total RNA was extracted from jejunal mucosa and synthesized for cDNA to determine the mRNA expression of the tight junction proteins and the inflammatory cytokines.【Result】 There was no significant difference in the indexes of organs among groups (P>0.05).Compared with NC group, the total antioxidant capacity (T-AOC) was significantly increased and the content of malondialdehyde (MDA) was significantly decreased by dietary BBR supplementation in jejunal mucosa of Yellow-feathered broilers at 63 d (P<0.05).The mRNA expression of Occludin and zonula occludens-1 (ZO-1) in jejunal mucosa was significantly higher in PC group compared with those in NC group and BBR groups at 21 d (P<0.05).Compared to NC and PC groups, the mRNA expression of Claudin-1 in the jejunal mucosa of Yellow-feathered broilers at 63 d was significantly higher in BBR group (P<0.05), and the mRNA expression of nucleotide-binding oligomerization domain-containing protein 1 (NOD1) at 21 and 42 d was significantly down-regulated in BBR groups (P<0.05).Moreover, the mRNA expression of interleukin-1β (IL-1β) and IL-8 (IL-8) of Yellow-feather broilers was decreased in BBR and PC groups when compared with PC at 42 d (P<0.05).Compared to NC group, the mRNA expression of tumor necrosis factor-α (TNF-α), IL-1β, and IL-8 was decreased in BBR group at 63 d (P<0.05).【Conclusion】 The addition of 250 mg/kg berberine to the diet could enhance the intestinal antioxidant capacity, improve the intestinal barrier and reduce the expression of intestinal inflammatory factors in Yellow-feathered broilers at 63 d, so as to improve the intestinal immune function.

Key words: berberine; Yellow-feathered broiler; intestinal barrier; gene expression; antioxidant capacity; intestinal immunity

中图分类号:

S831.5

朱翠, 黄凯勇, 李丽娜, 吴爱基, 吴鹏, 符芳苗, 罗苑华, 张辉华. 小檗碱对黄羽肉鸡器官指数、抗氧化能力和肠道免疫功能的影响[J]. 中国畜牧兽医, 2022, 49(3): 932-941.

ZHU Cui, HUANG Kaiyong, LI Lina, WU Aiji, WU Peng, FU Fangmiao, LUO Yuanhua, ZHANG Huihua. Effects of Berberine on Organ Index, Antioxidant Capacity and Intestinal Immunity in Yellow-feathered Broilers[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 932-941.

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