›› 2010, Vol. 37 ›› Issue (5): 133-136.

• 遗传繁育 • 上一篇    下一篇

培养时间和表皮生长因子对兔卵母细胞体外成熟的影响

徐贵江1,2，吴添文1，任丽伟1，李碧春1，吴信生1   

  1. （1.扬州大学动物科学与技术学院， 扬州 225009； 2.扬州市妇幼保健院， 扬州 225002）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-05-20 发布日期:2010-05-20
  • 通讯作者: 吴信生

Effect of Culture Time and Epidermal Growth Factor on the in vitro aturation of Rabbit Oocytes〖HJ1〗〖HJ〗〖STBZ〗〖WT5BZ〗〖JZ〗

XU Gui-jiang1,2, WU Tian-wen1, REN Li-wei1, LI Bi-chun1, WU Xin-sheng1   

  1. （1.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;2.Yangzhou Women and Children Healthcare Hospital, Yangzhou 225002, China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-05-20 Published:2010-05-20
  • Contact: WU Xin-sheng

摘要： 本试验旨在探讨培养时间和表皮生长因子（EGF）对兔卵母细胞体外成熟及受精的影响。分别用切割法和针刺挤压法取经超排后母兔卵巢表面的卵母细胞颗粒细胞复合体（COCs），将COCs分别在不同时间（24、30、36及40 h）和在基础培养液中添加不同浓度表皮生长因子（0、50、100 ng/mL）进行体外成熟培养并观察其第一极体排出率。结果表明：①针刺挤压法获得COCs数明显高于切割法（P<0.05）；②体外成熟培养时间30（36） h的第一极体排出率高于24（40） h（P<0.05）；③添加50和100 ng/mL EGF组卵母细胞第一极体排出率明显高于对照组（P<0.05）。因此，①兔卵巢卵母细胞的获得方法以针刺挤压法较好；②兔卵母细胞成熟时间以30~36 h为宜；③EGF对兔卵母细胞体外成熟有促进作用，在基础培养液中添加100 ng/mL EGF为宜。

关键词: 兔; 卵母细胞; 体外成熟; 表皮生长因子

Abstract: To study effect of culture time and epidermal growth factor on the in vitro maturation of rabbit oocytes. After superovulating female rabbit, we killed it and took out it’s ovarians, washed them and obtained COCs on the surface of ovarian by method of cutting with blade or by stab-extruding with niddle, observed the emission rate of the first polar body at different times (24, 30, 36 and 40 hours) and added in the basic culture medium with different concentrations (0, 50, 100 ng/mL) of epidermal growth factor (EGF) in vitro maturation. The results showed that： ①Method of stab-extruding with niddle to obtain the number of COCs was bitter than cutting with blade (P<0.05); ②the first polar body emission rate at in vitro maturation time of 30 (36) hours of was higher than 24 (40) hours (P<0.05); ③the first polar body emission rate in adding 50 ng/mL and 100 ng / mL EGF group was significantly higher (P<0.05). Therefore,
①Method of stab-extruding with niddle was better than method of cutting with blade in obtaining COCs on the surface of the rabbit ovarian；②On in vitro maturation of rabbit oocyte, time of 30 to 36 hours was appropriate；③EGF could significantly improve oocytes maturation, it was suitable to add 100 ng/mL EGF in the basic culture medium.

Key words: rabbit; oocyte; in vitro maturation; EGF

中图分类号: 

京ICP备10030713号-19
版权所有 © 2019 中国农业科学院北京畜牧兽医研究所
地址：北京市海淀区圆明园西路农大南路中国农业科学院北京畜牧兽医研究所　邮编：100193　
电话：010-62811226，62810371，62816020　E-mail：zgxmsy@caas.cn
本系统由北京玛格泰克科技发展有限公司设计开发