千金藤素对牛体外卵母细胞和早期胚胎抗凋亡能力的影响

doi:10.16431/j.cnki.1671-7236.2024.07.020

摘要/Abstract

摘要： 【目的】探究千金藤素(Ceph)对牛卵母细胞及早期胚胎抗凋亡能力的影响,为提高牛体外胚胎生产效率提供参考,同时为Ceph抗癌过程中的药理作用提供新的数据。【方法】将牛卵母细胞分别在含不同浓度(0、10、100、500、1 000 μmol/L)Ceph的体外成熟培养液中培养,观测卵母细胞成熟率及受精后的早期胚胎发育,确定Ceph的最适浓度,并对牛卵母细胞组织蛋白酶B(CB)活性、细胞自噬相关蛋白LC3水平、凋亡相关基因Survivin和Caspase-3 mRNA表达水平及囊胚细胞凋亡率进行检测。【结果】经 500 μmol/L Ceph处理的牛卵母细胞成熟率及早期胚胎分裂率、囊胚率均显著高于其他组(P＜0.05)。添加Ceph显著降低了牛卵母细胞CB活性和LC3水平(P＜0.05),显著提高了抗凋亡相关基因Survivin mRNA表达水平(P＜0.05),显著降低了促凋亡相关基因Caspase-3 mRNA表达水平及囊胚细胞凋亡率(P＜0.05)。【结论】在体外胚胎生产过程中添加Ceph能够通过调控凋亡相关基因Survivin和Caspase-3的表达影响CB和LC3水平,提升牛卵母细胞抗凋亡能力和体外胚胎生产效率。

关键词: 千金藤素; 牛; 卵母细胞; 早期胚胎; 抗凋亡

Abstract: 【Objective】 This study was aimed to explore the effect of cepharanthine (Ceph) on the antiapoptotic capacity of bovine oocytes and early embryos,so as to provide reference for improving the efficiency of bovine embryo production in vitro and new data for the pharmacological action of Ceph in anticancer process.【Method】 Bovine oocytes were cultured in in vitro mature medium containing different concentrations (0,10,100,500 and 1 000 μmol/L) of Ceph to observe the oocyte maturation rate and early embryo development after fertilization,the optimal concentration of Ceph was determined.The cathepsin B (CB) activity,the cell autophagy-related protein (LC3) level,the mRNA expression of apoptosis-related genes Survivin and Caspase-3,and the apoptosis rate of blastocysts in bovine oocyte were detected.【Result】 The maturation rate of bovine oocytes and the division rate of early embryo treated with 500 μmol/L Ceph were significantly higher than that of other groups (P＜0.05).The addition of Ceph significantly reduced the CB activity and LC3 level in bovine oocytes (P＜0.05),significantly increased the mRNA expression of Survivin gene (P＜0.05),and significantly reduced the mRNA expression of Caspase-3 gene and the apoptosis rate of blastocysts (P＜0.05).【Conclusion】 The addition of Ceph during in vitro embryo production could regulate the expression of apoptosis-related genes Survivin and Caspase-3 to affect the levels of CB and LC3,and enhance the anti-apoptotic capacity of bovine oocytes and in vitro embryo production efficiency.

Key words: cepharanthine; cattle; oocyte; early embryo; anti-apoptosis

中图分类号:

S814.8

郑湘民, 王玉琪, 徐冰洁, 孙朝阳, 高青山, 方南洙, 金庆国. 千金藤素对牛体外卵母细胞和早期胚胎抗凋亡能力的影响[J]. 中国畜牧兽医, 2024, 51(7): 2943-2952.

ZHENG Xiangmin, WANG Yuqi, XU Bingjie, SUN Zhaoyang, GAO Qingshan, FANG Nanzhu, JIN Qingguo. Effect of Cepharanthine on Anti-apoptotic Ability of Bovine Oocytes and Early Embryos in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(7): 2943-2952.

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