中国畜牧兽医 ›› 2024, Vol. 51 ›› Issue (3): 1171-1182.doi: 10.16431/j.cnki.1671-7236.2024.03.029
• 遗传繁育 • 上一篇
李有为1, 程亚倬2, 商继勇2, 张廷龙1, 孙铭菊2
收稿日期:
2023-08-12
发布日期:
2024-02-27
通讯作者:
孙铭菊
E-mail:sun8911@163.com
作者简介:
李有为,E-mail:liyouwei008@163.com。
基金资助:
LI Youwei1, CHENG Yazhuo2, SHANG Jiyong2, ZHANG Tinglong1, SUN Mingju2
Received:
2023-08-12
Published:
2024-02-27
摘要: 体外成熟是哺乳动物胚胎工程获得成熟卵母细胞的主要途径,卵母细胞质量直接影响早期胚胎发育,妊娠的建立及维持,胎儿的发育等。哺乳动物卵巢表面数量众多的小卵泡可以作为胚胎工程卵母细胞的来源,但从哺乳动物卵巢获取的卵母细胞成熟差异较大,存在处于发育各个时期的卵母细胞,小卵泡卵母细胞体外成熟质量差,发育能力不足。如何提高小卵泡卵母细胞体外成熟的发育能力以充分挖掘优良母畜的遗传资源,提高胚胎工程效率成为研究热点。基于此,文章概述哺乳动物小卵泡卵母细胞体外成熟的研究进展,比较大、小卵泡卵母细胞物质积累差异、卵泡的卵泡液成分及含量差异以及卵泡颗粒细胞和卵丘细胞的差异,分析哺乳动物小卵泡卵母细胞体外成熟质量差的原因,分析总结提高哺乳动物小卵泡体外成熟卵母细胞发育能力的措施,包括抑制核成熟、成熟前培养、颗粒细胞及卵丘细胞与卵母细胞共培养,期望能够为小卵泡卵母细胞发育调控机制的研究及发育能力的提高提供理论指导。
中图分类号:
李有为, 程亚倬, 商继勇, 张廷龙, 孙铭菊. 哺乳动物小卵泡卵母细胞体外成熟的研究进展[J]. 中国畜牧兽医, 2024, 51(3): 1171-1182.
LI Youwei, CHENG Yazhuo, SHANG Jiyong, ZHANG Tinglong, SUN Mingju. Research Progress on in vitro Maturation of Small Follicle-derived Oocytes in Mammalian[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 1171-1182.
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