›› 2010, Vol. 37 ›› Issue (9): 132-134.

• 遗传繁育 • 上一篇    下一篇

京海黄鸡IGFBP-3基因外显子1和内含子1部分序列多态性及其与生长繁殖性状的相关性

金崇富1,王金玉1,王慧华1,张跟喜1,施会强2,俞亚波2   

  1. (1.扬州大学动物科学与技术学院,扬州 225009; 2.江苏京海集团,南通 226103)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-09-20 发布日期:2010-09-20
  • 通讯作者: 王金玉

Genetic Diversity of IGFBP-3 Gene Exon 1 and Partial Sequence of Intron 1 and their Association with Growth and Reproductive

JIN Chong-fu1, WANG Jin-yu1,WANG Hui-hua1,ZHANG Gen-xi1,SHI Hui-qiang2,YU Ya-bo2   

  1. (1.College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China;2.Jiangsu Jinghai Poultry Group Co.Ltd.,Nantong 226103,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-09-20 Published:2010-09-20
  • Contact: WANG Jin-yu

摘要: 以京海黄鸡母鸡为材料,采用PCR-SSCP技术对IGFBP-3基因外显子1及内含子1部分序列多态位点进行研究,并计算基因型频率、基因频率、卡方值和部分遗传多态性指标。结果表明,在外显子1上没有检测到多态位点,在内含子1上检测到1个多态位点,该位点为中度多态,在160 bp处发生T到G的突变,导致BB、AB型的56日龄体重显著大于AA型(P<0.05),AA型的11月产蛋数显著大于BB、AB型(P<0.05)。由此初步推断,内含子1对产蛋及生长发育很可能有一定的促进作用,A为产蛋的优势基因,B为体重的优势基因。

关键词: 京海黄鸡; IGFBP-3; SNP位点; 多态性

Abstract:

To Jinghai yellow chicken hens as materialusing PCR-SSCP technique to study IGFBP-3 gene exon 1 and partial sequence of intron 1 polymorphic site and calculate the genotype, gene frequency, chi-square value and some indicators of genetic polymorphism. The results of exon 1 was not detected polymorphic site, in intron 1 of the partial sequence detected in more than one polymorphic site, the locus polymorphism as a moderate, in the first 160 bp took place T to G mutation, leading to BB and AB type 56 days of age, body weight was significantly greater than AA type (P<0.05), AA type egg in November was significantly greater than the number of BB and AB type (P<0.05). From this initial inference of intron 1 on the egg-laying and growth was likely to have a certain role in promoting, A gene for the advantage of egg-laying, B gene for the advantage of weight.

Key words: Jinghai yellow chicken; IGFBP-3 gene; SNP; polymorphisms

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