›› 2016, Vol. 43 ›› Issue (3): 784-790.doi: 10.16431/j.cnki.1671-7236.2016.03.032

• 遗传繁育 • 上一篇    下一篇

中国北方绵羊基因组拷贝数变异研究

侯成林1,2, 王伟1, 周欢敏1, 张焱如1, 曹俊伟1   

  1. 1. 内蒙古农业大学生命科学学院, 呼和浩特 010018;
    2. 包头轻工职业技术学院, 包头 014030
  • 收稿日期:2015-10-08 出版日期:2016-03-20 发布日期:2016-03-30
  • 通讯作者: 曹俊伟 E-mail:caojunwei1970@163.com
  • 作者简介:侯成林(1982-),男,内蒙古巴彦淖尔人,博士,讲师,研究方向:动物遗传与发育,E-mail:liru_826@sina.com
  • 基金资助:
    内蒙古农业大学校内基金

Study on Genome Copy Number Variation of Sheep in Northern China

HOU Cheng-lin1,2, WANG Wei1, ZHOU Huan-min1, ZHANG Yan-ru1, CAO Jun-wei1   

  1. 1. College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China;
    2. Baotou Light Industry Vocational Technical College, Baotou 014030, China
  • Received:2015-10-08 Online:2016-03-20 Published:2016-03-30

摘要: 为寻找绵羊基因组中可能的遗传性状相关标记,本试验采用比较基因组杂交(comparative genomic hybridization,CGH)芯片技术,构建了蒙古羊、哈萨克羊、藏羊的拷贝数变异(copy number variation,CNV)多样性图谱。试验结果显示,共检测出28个CNV区域(CNV region,CNVRs),包括11个扩增型、15个缺失型和2个扩增—缺失型。通过功能注释和代谢通路分析发现,在蒙古羊和藏羊基因组中血红蛋白基因存在拷贝数扩张,可能与两种绵羊长期生活在高原低氧环境中产生的适应性有关。对CNVRs和CNV相关基因进行实时荧光定量PCR检验,83.3%的实时荧光定量PCR结果与芯片检测结果一致。通过对中国北方3种绵羊的基因组CNV的研究,为不同绵羊品种间遗传变异的研究奠定了基础。

关键词: 绵羊; 拷贝数变异; 比较基因组杂交; 实时荧光定量PCR

Abstract: To detect the association of the biological traits and genetic properties in sheep genome,array comparative genomic hybridization (aCGH) system was used to identify the CNVs in the sheep genome and the CNVs map was constructed in Mongolian sheep,Kazakh sheep and Tibetan sheep.The results showed that 28 CNV regions (CNVRs) were found,containing 11 gains,15 losses and 2 gain-losses.The HBB gene was amplified in Mongolian sheep and Tibetan sheep,which might be attributed to adaptability in low oxygen and high altitude environment.Real-time PCR was performed for CNVRs and CNV genes,83.3% of Real-time RCR results were consistent with the CGH.The study that performed the genome-wide detection of copy number variations of sheep in Northern China,would provid foundation for studying genetic variation in different sheep breeds.

Key words: sheep; copy number variation; comparative genomic hybridization; Real-time PCR

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