雌激素对奶牛输卵管组织COX-1和COX-2表达的影响

doi:10.16431/j.cnki.1671-7236.2016.03.033

摘要/Abstract

摘要： 试验旨在阐明雌激素(E₂)对体外培养的奶牛输卵管组织中环氧合酶-1(cyclooxygenase-1,COX-1)与环氧合酶-2(cyclooxygenase-2,COX-2)基因表达的影响。分离培养奶牛输卵管组织,用不同浓度的E₂作用于奶牛输卵管组织,采用实时荧光定量PCR与Western blotting检测COX-1与COX-2 mRNA和蛋白的表达量。结果表明,E₂作用浓度为10^-11 mol/L时奶牛输卵管组织中的COX-1与COX-2 mRNA相对表达量达到高峰;E₂作用时间为8 h时COX-1 mRNA的相对表达量达到高峰,E₂作用时间为2 h时COX-2 mRNA的相对表达量达到高峰;浓度为10^-11 mol/L的E₂作用不同时间后,COX-1蛋白的相对表达量在8~48 h显著升高;没有检测到COX-2蛋白的表达。

关键词: 输卵管; 环氧合酶-1; 环氧合酶-2; 雌激素

Abstract: The aim of this study was to clarify the effects of estrogen (E₂) on cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) gene expression in the cow oviduct tissue in vitro.Quantitative Real-time PCR and Western blotting were used to analyze the mRNA and protein expression of COX-1 and COX-2 at different time points after different concentrations of E₂ treatment.The results indicated that the relative expression of COX-1 and COX-2 mRNA reached the highest levels with 10^-11 mol/L E₂ treatment,the relative expression of COX-1 mRNA reached the highest level at 8 h after treatment;The relative expression of COX-2 mRNA reached the highest level at 2 h after treatment.The relative expression of COX-1 protein increased significantly at the range from 8 to 48 h after E₂ treatment.However,we did not detect COX-2 protein expression.

Key words: oviduct; cyclooxygenase-1; cyclooxygenase-2; estrogen (E₂)

中图分类号:

Q786

沈媛, 黄娜, 刘博, 毛伟, 曹金山. 雌激素对奶牛输卵管组织COX-1和COX-2表达的影响[J]. , 2016, 43(3): 791-797.

SHEN Yuan, HUANG Na, LIU Bo, MAO Wei, CAO Jing-shan. Effects of Estrogen on COX-1 and COX-2 Expression in Bovine Oviduct Smooth Muscle[J]. , 2016, 43(3): 791-797.

参考文献

[1] Coleman R A,Smith W L.International union of pharmacology classification of prostanoid receptors:Properties,distribution,and structure of the receptors and their subtypes[J].Pharmacol Rev,1994,46:205-229.
[2] Challis J R G,Olson D M.The Physiology of Reproduction[M].New York:Raven Press,1988.
[3] Chan W Y.Uterine and placental prostaglandins and their modulation of oxytocin sensitivity and contractility in the parturient uterus[J].BiolReprod,1983,29:680-688.
[4] Peri K G,Quiniou C.A novel selective FP antagonist that delays preterm labor[J].Semin Perinatol,2002,26:389-397.
[5] Lee S H,Soyoola E,Chanmugam P,et al.Selective expression of mitogen-inducible cyclooxygenase in macrophages stimulated with lipopolysaccharide[J].Biol Chem,1992,267:25934-25938.
[6] Reddy S T,Herschman H R.Ligand-induced prostaglandin synthesis requires expression of the TIS10/PG-2 prostaglandin synthase gene in murine fibroblasts and macrophages[J].Biol Chem,1994,269:15473-15480.
[7] O'Sullivan M G,Huggins E M Jr,Meade E A,et al.Lipopolysaccharide induces prostaglandin H synthase-2 in alveolar macrophages[J].Biochem Biophys Res Commun,1992,187:1123-1127.
[8] Harper M J K.The Physiology of Reproduction[M].New York:Raven Press,1994.
[9] 王艳萍,曾维斌.奶牛繁殖障碍防治的研究进展[J].动物疫病防制,2008,112:45-47.
[10] Ospina J A,Krause D N.17β-estradiol increases rat cerebrovascular prostacyclin synthesis by elevating cyclooxygenase-1 and prostacyclin synthase[J].Stroke,2002,33:600-605.
[11] Lu B,Jiang Y J,Choy P C.17-βestradiol enhances prostaglandin E₂ production in human U937-derived macrophages[J].Molecular and Cellular Biochemistry,2004,262(1-2):101-110.
[12] Gabler C,Odau S,Muller K,et al.Exploring cumulus-oocyte-complex-oviductal cell interactions:Gene profiling in the bovine oviduct[J].Journal of Physiology and Pharmacology:An Official Journal of the Polish Physiological Society,2008,59(9):29-42.
[13] Lim H.Multiple female reproductive failures in cyclooxygenase 2-deficient mice[J].Cell,1997,91(2):197-208.
[14] Wijayagunawardane M P B,Choi Y H,Miyamoto A,et al.Effect of ovarian steroids and oxytocin on the production of prostaglandin E₂,prostaglandin F₂ alpha and endothelin-1 from cow oviductal epithelial cell monolayers in vitro[J].Animal Reproduction Science,1999,56(1):11-17.
[15] Eberhart C E,Coffey R J,Radhika A,et al.Up-regulation of cyclooxygenase 2 gene expression in human colorectal adenomas and adenocarcinomas[J].Gastroenterology, 1994,107(4):1183-1188.
[16] Herschman H R.Prostaglandin synthase 2[J].Biochimica et Biophysica Acta,1996,1299(1):125-140.
[17] Kargman S L,O'neill G P,Vickers P J,et al.Expression of prostaglandin G/H synthase-1 and-2 protein in human colon cancer[J].Cancer Research,1995,55(12):2556-2559.
[18] Hla T,Neilson K.Human cyclooxygenase-2 cDNA[J].Proceedings of the National Academy of Sciences of the United States of America,1992,89(16):7384-7388.
[19] Subbaramaiah K,Telang N,Ramonetti J T,et al.Transcription of cyclooxygenase-2 is enhanced in transformed mammary epithelial cells[J].Cancer Research,1996,56(19):4424-4429.
[20] DuBois R N,Radhika A,Reddy B S,et al.Increased cyclooxygenase-2 levels in carcinogen-induced rat colonic tumors[J].Gastroenterology,1996,110(4):1259-1262.
[21] Boolbol S K,Dannenberg A J,Chadburn A,et al.Cyclooxygenase-2 overexpression and tumor formation are blocked by sulindac in a murine model of familial adenomatous polyposis[J].Cancer Research,1996,56(11):2556-2560.
[22] 武岳.17-β-雌二醇和孕酮对体外培养奶牛输卵管上皮细胞中环氧合酶表达影响[D].呼和浩特:内蒙古农业大学,2010.
[23] 苏日娜,武岳,黄娜.奶牛输卵管平滑肌前列腺素类受体的分析研究[J].中国畜牧兽医,2010,31(9):118-123.
[24] Odau S,Gabler C,Holder C,et al.Differential expression of cyclooxygenase 1 and cyclooxygenase 2 in the bovine oviduct[J].The Journal of Endocrinology,2006,191(1):263-274.
[25] Sirois J,Simmons D L,Richards J S.Hormonal regulation of messenger ribonucleic acid encoding a novel isoform of prostaglandin endoperoxide H synthase in rat preovulatory follicles.Induction in vivo and in vitro[J].The Journal of Biological Chemistry,1992,267(16):11586-11592.
[26] Sirois J.Induction of prostaglandin endoperoxide synthase-2 by human chorionic gonadotropin in bovine preovulatory follicles in vivo[J].Endocrinology,1994,135(3):841-848.
[27] Kennedy T G.Prostaglandin E2,adenosine 3':5'-cyclic monophosphate and changes in endometrial vascular permeability in rat uteri sensitized for the decidual cell reaction[J].Biology of Rproduction,983,29(5):1069-1076.
[28] Rankin J C,Ledford B E,Baggett B.Early involvement of cyclic nucleotides in the artificially stimulated decidual cell reaction in the mouse uterus[J].Biology of Reproduction,1977,17(4):549-554.
[29] 黄娜.雌激素对奶牛输卵管平滑肌前列腺素E₂和F_2α合成及其受体表达调控的影响[D].呼和浩特:内蒙古农业大学,2015.