前列腺素E2受体激动剂和雌激素对奶牛输卵管上皮细胞中转化生长因子β3表达的影响

doi:10.16431/j.cnki.1671-7236.2015.06.002

《中国畜牧兽医》---唯一指定的官方网站 ›› 2015, Vol. 42 ›› Issue (6): 1333-1339.doi: 10.16431/j.cnki.1671-7236.2015.06.002

前列腺素E₂受体激动剂和雌激素对奶牛输卵管上皮细胞中转化生长因子β₃表达的影响

王毛毛^1,2, 毛伟^1,2, 曹金山^1,2, 董至恒^1,2, 刘博^1,2

1. 内蒙古农业大学兽医学院, 呼和浩特 010018;
2. 农业部动物疾病临床诊疗技术重点实验室, 呼和浩特 010018

收稿日期:2014-12-25 出版日期:2015-06-20 发布日期:2015-07-23
通讯作者: 曹金山 E-mail:jinshancao@imau.edu.cn
作者简介:王毛毛(1988-),女,内蒙古二连浩特人,硕士生,研究方向:兽医药理学与毒理学,E-mail:970096059@qq.com
基金资助:
国家自然科学基金(31160490);新世纪优秀人才计划支持项目(NCET-11-109)

Effect of PGE₂ Receptor Agonist Butaprost and Estrogen on Expression of TGFβ₃ in Bovine Oviduct Epithelial Cell

WANG Mao-mao^1,2, MAO Wei^1,2, CAO Jin-shan^1,2, DONG Zhi-heng^1,2, LIU Bo^1,2

1. College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Key Laboratory of Animal Disease Clinical Diagnosis, Ministry of Agriculture, Hohhot 010018, China

Received:2014-12-25 Online:2015-06-20 Published:2015-07-23

摘要/Abstract

摘要： 本试验旨在观察前列腺素E₂受体激动剂(布他前列腺素(butaprost))与雌激素对奶牛输卵管上皮细胞中转化生长因子β₃(TGFβ₃)表达的影响,阐明butaprost和雌激素对奶牛输卵管上皮细胞TGFβ₃有无协同调控作用.采用胰酶消化法及机械法分离培养奶牛输卵管上皮细胞,分别将butaprost和雌激素作用于体外培养的奶牛输卵管上皮细胞,采用实时荧光定量PCR技术检测butaprost和雌激素对奶牛输卵管上皮细胞中TGFβ₃ mRNA表达的影响.结果显示,与0 h作用组相比,雌激素作用16、24和48 h时对奶牛输卵管上皮细胞TGFβ₃的表达量均极显著升高(P< 0.01),4 h的表达量极显著降低(P< 0.01);且受体激动剂butaprost和雌激素有协同调控TGFβ₃的效应;加入吲哚美辛后能有效抑制内源性前列腺素对TGFβ₃表达的作用.结果表明,butaprost和雌激素可调控奶牛输卵管上皮细胞TGFβ₃ mRNA 的表达.

关键词: 布他前列腺素(butaprost); 雌激素; 奶牛输卵管上皮细胞; 转化生长因子β ₃(TGFβ ₃)

Abstract: This study was aimed to observe effect of the prostaglandin receptor agonists butaprost and estrogen on TGFβ₃ mRNA expression and illustrate whether butaprost and estrogen had coordinated regulation on bovine oviduct epithelial cells (BOECs) transforming growth factor β₃ (TGFβ₃) or not.BOECs were isolated and cultured by mechanical and pancreatic enzyme digestion method;butaprost and estrogen acted on BOECs,respectively;mRNA expression of TGFβ₃ was detected by Real-time PCR in BOECs.The results showed that after the action of estrogen,the expression of TGFβ₃ at 16,24 and 48 h compared with 0 h were extremely significantly increased (P< 0.01),TGFβ₃ mRNA expression reached minimal at 4 h (P< 0.01);The receptor agonist butaprost and estrogen had coordinated regulation on TGFβ₃.Adding indomethacin could inhibit the expression of endogenous prostaglandin for TGFβ₃.The results showed that butaprost and estrogen could regulate the expression of TGFβ₃ mRNA in BOECs.

Key words: butaprost; estrogen; bovine oviduct epithelial cells (BOECs); transforming growth factor β₃ (TGFβ₃)

中图分类号:

S823

王毛毛, 毛伟, 曹金山, 董至恒, 刘博. 前列腺素E₂受体激动剂和雌激素对奶牛输卵管上皮细胞中转化生长因子β₃表达的影响[J]. 《中国畜牧兽医》---唯一指定的官方网站, 2015, 42(6): 1333-1339.

WANG Mao-mao, MAO Wei, CAO Jin-shan, DONG Zhi-heng, LIU Bo. Effect of PGE₂ Receptor Agonist Butaprost and Estrogen on Expression of TGFβ₃ in Bovine Oviduct Epithelial Cell[J]. , 2015, 42(6): 1333-1339.

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前列腺素E₂受体激动剂和雌激素对奶牛输卵管上皮细胞中转化生长因子β₃表达的影响

Effect of PGE₂ Receptor Agonist Butaprost and Estrogen on Expression of TGFβ₃ in Bovine Oviduct Epithelial Cell

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