基于线粒体Cytb基因的凉山黑绵羊群体遗传多样性分析

doi:10.16431/j.cnki.1671-7236.2023.09.023

摘要/Abstract

摘要： 【目的】研究四川省凉山黑绵羊群体的遗传多样性和亲缘关系,以期为当地黑绵羊种质资源保护及合理利用提供科学依据。【方法】利用PCR法扩增凉山黑绵羊线粒体细胞色素b(Cytb)基因并测序,通过BLAST比对确认序列长度和突变位点信息,结合从GenBank中下载的国内外22个绵羊品种的153条绵羊线粒体Cytb基因序列,用Mega 7.0软件进行碱基组成和遗传距离分析,并利用DnaSP 5.0软件进行遗传多样性分析。【结果】凉山黑绵羊群体Cytb基因大小为1 140 bp,未发现插入或缺失,AT含量高于GC含量;采集的60只黑绵羊的Cytb基因共存在13个变异位点,有13种单倍型,其中H2、H3为优势单倍型;凉山黑绵羊单倍型多样性(Hd)、核苷酸多样性(Pi)和平均核苷酸变异数(K)分别为0.674、0.00120和1.371。系统发育树分析结果表明,凉山黑绵羊与乌拉黑羊遗传距离最小,与盘羊遗传距离最大。【结论】凉山州本地黑绵羊Cytb基因遗传多样性较低,其中盐源黑绵羊群体Cytb基因遗传多样性远低于布拖、普格黑绵羊群体,3个黑绵羊群体之间的亲缘关系较近,初步推测具有2个母系起源。凉山州本地黑绵羊群体与乌拉黑羊等亲缘关系最近,与盘羊亲缘关系较远。

关键词: 凉山黑绵羊; Cytb基因; 遗传多样性; 系统进化

Abstract: 【Objective】 This study was amied to evalute the genetic diversity and genetic relationship of Liangshan black sheep population in Sichuan province,in order to provide scientific basis for the protection and rational utilization of local black sheep germplasm resources.【Method】 Mitochondrial cytochrome b (Cytb) gene of Liangshan black sheep was amplified by PCR and sequenced.The sequence length and mutation site information were confirmed by BLAST comparison.Combined with 153 sequences from 22 sheep breeds at home and abroad downloaded from GenBank,mitochondrial Cytb gene sequences of sheep were analyzed using Mega 7.0 software for base composition and genetic distance analysis,and DnaSP 5.0 software was used for genetic diversity analysis.【Result】 The Cytb gene size of Liangshan black sheep population was 1 140 bp,no insertion or deletion was found,and the AT content was higher than GC content.A total of 13 variation sites were found in the Cytb gene of 60 black sheep,with 13 haplotypes,of which H2 and H3 were the dominant haplotypes.The haplotype diversity (Hd),nucleotide diversity (Pi) and mean nucleotide variance (K) of Liangshan black sheep were 0.674,0.00120 and 1.371, respectively.Phylogenetic tree analysis showed that the genetic distance between Liangshan black sheep and Wula Black sheep was the smallest,and the genetic distance between Liangshan black sheep and Ovis ammon was the largest.【Conclusion】 The genetic diversity of Cytb gene in Liangshan local black sheep was low,and the genetic diversity of Cytb gene in Yanyuan black sheep was much lower than that in Butuo and Puge black sheeps.The three black sheep groups were closely related,and it was speculated that they had two matrilineal origins.Liangshan local black sheep had the closest relationship with Wula black sheep,but had a distant relationship with Ovis ammon.

Key words: Liangshan black sheep; Cytb gene; genetic diversity; phylogenetic

中图分类号:

S826.8⁺9

引用本文

乐云峰, 黄卫平, 刘敏, 金素钰, 黄林, 郑玉才. 基于线粒体Cytb基因的凉山黑绵羊群体遗传多样性分析[J]. 中国畜牧兽医, 2023, 50(9): 3671-3679.

YUE Yunfeng, HUANG Weiping, LIU Min, JIN Suyu, HUANG Lin, ZHENG Yucai. Genetic Diversity Analysis of Liangshan Black Sheep Population Based on Mitochondrial Cytb Gene[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3671-3679.

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参考文献

[1] 王毅,刘晓波,何子拉,等.凉山州黑绵羊种公羊饲养管理[J].中国畜禽种业,2020,16(10):101. WANG Y,LIU X B,HE Z L,et al.Breeding and management of black sheep breeding rams in Liangshan Yi Autonomous Prefecture[J].The Chinese Livestock and Poultry Breeding,2020,16(10):101.(in Chinese)
[2] 沙马阿木.布拖黑绵羊产业保护与发展的调研[J].农家参谋,2019,616(8):149. SHAMA A M.Investigation on the protection and development of Butuo Black sheep industry[J].The Farmers Consultant,2019,616(8):149.(in Chinese)
[3] 王毅,刘晓波,何子拉,等.凉山州地方品种黑绵羊资源调查初报[J].中国畜禽种业,2021,17(3):2. WANG Y,LIU X B,HE Z L,et al.A preliminary report on the resources of local black sheep in Liangshan Yi Autonomous Prefecture[J]The Chinese Livestock and Poultry Breeding,2021,17(3):2.(in Chinese)
[4] GYLLENSTEN U,WHARTON D,WILSON A C.Maternal inheritance of mitochondrial DNA during backcrossing of two species of mice[J]. Journal of Heredity,1985(5):321-324.
[5] 陈晓勇,郭伟涛,赵兴波.哺乳动物线粒体基因组转录及其调控机制[J].中国草食动物科学,2013,33(2):53-58. CHEN X Y,GUO W T,ZHAO X B.Mechanism of mitochondrial genome transcription and its regulation in mammals[J].China Herbivore Science,2013,33(2):53-58.(in Chinese)
[6] 唐修君,贾晓旭,樊艳凤,等.基于Cytb基因全序列4个鸡品种遗传多态性和起源[J].农业生物技术学报,2020,28(6):1030-1038. TANG X J,JIA X X,FAN Y F,et al.Genetic diversity and origins of four chicken (Gallus domesticus) breeds based on full sequence of Cytb gene[J].Chinese Journal of Agricultural Biotechnology,2020,28(6):1030-1038.(in Chinese)
[7] 亓鹏.利用mtDNA D-loop序列多态性挖掘四川绵羊遗传资源[D].成都:四川农业大学,2016. QI P.The application of mtDNAD-loop sequence polymorphism insearching for sheep genetic resources in Sichuan province[D].Chengdu:Sichuan Agricultural University,2016.(in Chinese)
[8] 周明亮,陈明华,庞倩,等.布拖黑绵羊微卫星标记遗传多样性研究[J].草学,2018,2:74-80. ZHOU M L,CHEN M H,PANG Q,et al.Genetic diversity of microsatellite markers in black sheep in Butuo county[J].Journal of Grassland and Forage Science,2018,2:74-80.(in Chinese)
[9] AMILLS M,FRANCINO O,JANSA M,et al.Isolation of genomic DNA from milk samples by using Chelex resin[J].Journal of Dairy Research,1997,64:231-238.
[10] 陈天莹,胡峻,李孟纯,等.基于线粒体Cytb基因探讨大尾羊与国内外绵羊品种的系统发生关系[J].甘肃畜牧兽医,2021,51(2):6. CHEN T Y,HU J,LI M C,et al.Study on the phylogenetic relationship between big tail sheep and sheep breeds at home and abroad based on mitochondrial Cytb gene[J].Gansu Animal Husbandry and Veterinary,2021,51(2):6.(in Chinese)
[11] WANG X,MA Y H,CHEN H.Analysis of the genetic diversity and the phylogenetic evolution of Chinese sheep based on Cytb gene sequences[J].Acta Genetica Sinica,2006,33(12):1081-1086.
[12] LV F H,PENG W F,YANG J,et al.Mitogenomic meta-analysis identifies two phases of migration in the history of Eastern Eurasian sheep[J].Oxford University Press,2015,32(10):2515-2533.
[13] 成述儒,韩建林,OLIVIER H,等.中国绵羊群体mtDNA D-loop的遗传多样性分析[J].甘肃农业大学学报,2005,4:440-447. CHENG S R,HAN J L,OLIVIER H,et al.Genetic diversity analysis of mtDNA D-loop in Chinese sheep population[J].Journal of Gansu Agricultural University,2005,4:440-447.(in Chinese)
[14] 陈惟昌,陈志华,王自强,等.线粒体遗传密码及基因组遗传密码的对称分析[J].生物物理学报,2002,18(1):87-94. CHEN W C,CHEN Z H,WANG Z Q,et al.Symmetrical analysis of mitochondrial genetic code and genomic genetic code[J].Acta Biophysica Sinica,2002,18(1):87-94.(in Chinese)
[15] 宁礼群,古丽娜·艾山,马合木提·哈力克.新疆地方绵羊Cytb基因遗传多样性与起源研究[J].西南农业学报,2016,29(12):3001-3008. NING L Q,GULINA A,MAHEMUTI G.Analysis of genetic diversity and phylogenetic relationship of Xinjiang indigenous sheep based on Cytb gene[J].Southwest China Journal of Agricultural Sciences,2016,29(12):3001-3008.(in Chinese)
[16] 刘刚,杜玲,陆健,等.中国西南地区主要地方绵羊Cytb基因遗传多样性及系统进化的研究[J].农业生物技术学报,2016,5:678-688. LIU G,DU L,LU J,et al.The genetic diversity and phylogenetic relationship among Chinese main indigenous sheep (Ovis aries) in Southwest regions based on Cytb gene[J].Journal of Agricultural Biotechnology,2016,5:678-688.(in Chinese)
[17] JIA Y,LI R,CONGA R,et al.Maternal low-protein diet affects epigenetic regulation of hepatic mitochondrial DNA transcription in a sex-specific manner in newborn piglets associated with GR binding to its promoter[J].PLoS One,2013,8(5):e63855.
[18] ALONSO R A,ULLOA-ARVIZU R,GAYOSSO-VÁZQUEZ A.Mitochondrial DNA sequenceanalysis of the Mexican Creole sheep (Ovis aries) reveals a narrow Iberian maternal origin[J].Mitochondrial DNA Part A,2017,28(6):793-800.
[19] KOCHER T D,THOMAS W K,MEYER A,et al.Dynamics of mitochondrial DNA evolution in animals:Amplification and sequencing with conserved primers[J]. Proceedings of the National Academy of Sciences of the United States of America, 1989,86(16):6196-6200.
[20] IRWIN D M,KOCHER T D,WILSON A C.Evolution of the cytochrome b gene of mammals[J]. Journal of Molecular Evolution,1991,32(2):128-144.
[21] OREKHOV V,POLTORAUS A,ZHIVOTOVSKY L A,et al.Mitochondrial DNA sequence diversity in Russians[J].FEBS Letters,1999,445(1):197-201.
[22] SOLIMAN T,ALY W,FAHIM R M,et al.Comparative population genetic structure of redbelly tilapia (Coptodon zillii (Gervais,1848)) from three different aquatic habitats in Egypt[J].Ecology and Evolution,2017,7(24):11092-11099.
[23] SUN T,WANG S,HANIF Q,et al.Genetic diversity of mitochondrial cytochrome b gene in swamp buffalo[J].Animal Genetics,2020,51(6):977-981.
[24] 曹丽荣,王小明,方盛国.从细胞色素b基因全序列差异分析岩羊和矮岩羊的系统进化关系[J].动物学报,2003,2:198-204. CAO L R,WANG X M,FANG S G.A molecular phylogeny of Bharal and Dwarf blue sheep based on mitochondrial cytochrome b gene sequences[J].Acta Zoologica Sinica,2003,2:198-204.(in Chinese)
[25] 亓鹏,吴登俊.四川省两个绵羊群体遗传新资源的鉴定[J].黑龙江畜牧兽医,2017,12:119-122. QI P,WU D J.Identification of new genetic resources of two sheep populations in Sichuan province[J].Heilongjiang Animal Science and Veterinary Medicine,2017,12:119-122.(in Chinese)

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基于线粒体Cytb基因的凉山黑绵羊群体遗传多样性分析

Genetic Diversity Analysis of Liangshan Black Sheep Population Based on Mitochondrial Cytb Gene

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[1]	罗世民, 李中波. 怀化地区犬瘟热病毒H、F基因遗传多样性及其遗传进化分析[J]. 中国畜牧兽医, 2023, 50(8): 3248-3257.
[2]	叶雯, 孙东晓, 韩博. 全基因组重测序在畜禽研究领域的应用进展[J]. 中国畜牧兽医, 2023, 50(10): 4125-4132.
[3]	龚文滔, 潘向春, 蔡佳丽, 王祎菲, 李加琪, 张哲, 袁晓龙. 猪DNA甲基转移酶基因家族进化分析[J]. 中国畜牧兽医, 2022, 49(9): 3283-3291.
[4]	熊陈勇, 尹彦文, 施开创, 李军, 郑敏, 韦显凯, 冯淑萍, 龙凤, 屈素洁, 陆文俊, 周洪槿, 黄海莲, 谢守玉, 黎宗强. 2019-2021年鸭坦布苏病毒广西流行毒株遗传多样性分析[J]. 中国畜牧兽医, 2022, 49(8): 3099-3111.
[5]	彭彩淳, 李斌强, 王野影, 粟海军. 基于线粒体Cytb基因的贵州野猪(Sus scrofa) 群体遗传多样性分析[J]. 中国畜牧兽医, 2022, 49(7): 2601-2612.
[6]	张强, 洛桑顿珠, 巴桑旺堆, 彭阳洋, 俄广鑫, 尼玛次仁, 索朗多吉, 巴多, 旦巴, 鲜莉莉, 旦巴加参, 支张, 平措占堆. 利用微卫星遗传标记评估5个西藏牦牛群体遗传多样性及群体结构[J]. 中国畜牧兽医, 2022, 49(6): 2228-2238.
[7]	郭銮英, 李家磊, 金芹芹, 马骏, 刘全. 牛丙型肝炎病毒研究进展[J]. 中国畜牧兽医, 2022, 49(5): 1994-2000.
[8]	刘晓玮, 李瑞香, 王兴龙, 李晓. 基于mtDNA Cytb基因和D-loop区序列的陕西林麝遗传多样性分析[J]. 中国畜牧兽医, 2022, 49(4): 1352-1363.
[9]	徐民生, 柯海意, 杨冬霞, 施科达, 孙泽仪, 牛佳伟, 常鑫, 翟少伦, 臧莹安, 李春玲. 多重耐药胸膜肺炎放线杆菌GD2107株全基因组测序及生物信息学分析[J]. 中国畜牧兽医, 2022, 49(10): 4019-4031.
[10]	李中波, 侯强红. 湖南省怀化地区白纹伊蚊核糖体18S rRNA和线粒体cox1基因的序列测定及分析[J]. 中国畜牧兽医, 2021, 48(8): 2736-2745.
[11]	房瑞新, 田雪琪, 邹晨, 董依萌, 李洋, 邢秀梅, 刘欣. 利用Y染色体SRY基因分析马鹿的父系起源和遗传多样性[J]. 中国畜牧兽医, 2021, 48(7): 2457-2466.
[12]	李广祯, 马志杰, 陈生梅, 尕布藏, 李瑞哲. 基于mtDNA D-loop序列探究门源白牦牛与天祝白牦牛间的遗传差异[J]. 中国畜牧兽医, 2021, 48(5): 1647-1654.
[13]	肖逸, 王孟文, 张秀英, 古丽扎尔·阿不都克力木, 韩建林, 王玉涛. 基于mtDNA Cytb基因的马可·波罗盘羊遗传多样性与起源进化分析[J]. 中国畜牧兽医, 2021, 48(5): 1681-1690.
[14]	房瑞新, 田雪琪, 邹晨, 董依萌, 刘欣, 邢秀梅. 马鹿的起源进化与遗传多样性研究进展[J]. 中国畜牧兽医, 2021, 48(12): 4575-4587.
[15]	汤也, 陈怡, 田晓彦, 吕海峰, 甘军纪. 犬冠状病毒JS1706和JS1712毒株基因组3'端分子特性分析[J]. 中国畜牧兽医, 2021, 48(11): 4231-4241.