怀化地区犬瘟热病毒H、F基因遗传多样性及其遗传进化分析

doi:10.16431/j.cnki.1671-7236.2023.08.022

摘要/Abstract

摘要： 【目的】探究怀化地区犬瘟热病毒的基因变异与遗传多样性情况,阐明其遗传进化关系。【方法】从怀化地区收集30株犬源犬瘟热病毒样本,用PCR法扩增其H、F基因序列。利用DNAStar软件分析H、F基因序列碱基组成;利用Clustal X、MegAlign软件进行变异位点和遗传多样性分析;运用Clustal X、PhyML 3.0软件,采用最大似然法(ML)对怀化地区犬瘟热病毒进行遗传进化分析,并用FigTree v 1.3.1软件构建遗传进化树。【结果】30株怀化地区犬瘟热病毒均为Asia-Ⅰ型,其H、F基因序列长度分别为1 778和1 850 bp,其AT含量分别为56.8%~57.9%和54.9%~56.1%,GC含量分别为42.2%~43.0%和44.1%~44.9%。H基因的种内差异为0~3.1%,种间差异为51.36%~60.15%;F基因的种内差异为0~2.4%,种间差异为39.60%~53.09%。基于H、F基因序列所构建的遗传进化树发现,来源于怀化地区的30株犬源犬瘟热病毒全部位于遗传进化树的同一分支上。【结论】来源于怀化地区犬瘟热病毒之间虽存在一定程度的基因变异和遗传多样性,但它们之间的亲缘关系较近。且其H、F基因序列的种内差异小、种间差异大,是研究犬瘟热病毒基因变异、遗传多样性和遗传进化的重要遗传标记。

关键词: 犬瘟热病毒; H基因; F基因; 基因变异; 遗传多样性

Abstract: 【Objective】 The aim of this study was to explore the genetic variation and genetic diversity of Canine distemper virus in Huaihua district and clarify its genetic evolutionary relationship.【Method】 Thirty Canine distemper virus samples were collected from Huaihua district and H and F gene sequences were amplified by PCR.The H and F gene sequences were analyzed by DNAStar software.Clustal X and MegAlign software were used to analyze variation sites and genetic diversity.Clustal X and PhyML 3.0 software were used to analyze the genetic evolution of Canine distemper virus in Huaihua area by maximum likelihood method (ML),and the genetic evolution tree was constructed by FigTree v 1.3.1 software.【Result】 Thirty Canine distemper viruses in Huaihua were Asian-type Ⅰ,and the H and F gene sequences were 1 778 and 1 850 bp,respectively.The contents of AT were 56.8%-57.9% and 54.9%-56.1%,and the contents of GC were 42.2%-43.0% and 44.1%-44.9%,respectively.The intraspecific and interspecific differences of H gene ranged from 0 to 3.1% and from 51.36% to 60.15%.The intraspecific difference of F gene was 0-2.4%,and the interspecific difference was 39.60%-53.09%.Based on the genetic evolutionary tree constructed by H and F gene sequences,it was found that 30 Canine distemper viruses from Huaihua were all located in the same branch of the genetic evolutionary tree.【Conclusion】 Canine distemper viruses from Huaihua were closely related to each other although there was a certain degree of genetic variation and genetic diversity among them.In addition,the H and F gene sequences of Canine distemper virus showed little intraspecific difference but great interspecific difference,which was an important genetic marker for studying gene variation,genetic diversity and genetic evolution of Canine distemper virus.

Key words: Canine distemper virus; H gene; F gene; gene variation; genetic diversity

中图分类号:

S852.65⁺5

罗世民, 李中波. 怀化地区犬瘟热病毒H、F基因遗传多样性及其遗传进化分析[J]. 中国畜牧兽医, 2023, 50(8): 3248-3257.

LUO Shimin, LI Zhongbo. Genetic Diversity and Genetic Evolution of H and F genes of Canine Distemper Virus in Huaihua District[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3248-3257.

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