黄芪多糖对猪精液冷冻保存效果的影响

中国畜牧兽医 ›› 2014, Vol. 41 ›› Issue (9): 179-183.

黄芪多糖对猪精液冷冻保存效果的影响

杨尚雪^1,2, 潘天彪¹, 黄明光¹, 许春荣³, 刘德玉³, 王英群³, 李珣², 胡传活²

1. 广西畜牧研究所, 广西家畜遗传改良重点实验室, 广西南宁 530001;
2. 广西大学动物科技学院, 广西南宁 530005;
3. 广西畜禽品种改良站, 广西南宁 530001

收稿日期:2014-02-07 出版日期:2014-09-20 发布日期:2014-09-24
通讯作者: 胡传活 E-mail:hch64815@gxu.edu.cn
作者简介:杨尚雪（1989- ），女，河南人，硕士生，研究方向：动物解剖学及组织胚胎学。
基金资助:
广西家畜遗传改良重点实验室开放基金（2013GXKFKT-07）。

Effects of Astragalus Polysaccharides on the Quality of Frozen-thawed Boar Semen

YANG Shang-xue^1,2, PAN Tian-biao¹, HUANG Ming-guang¹, XU Chun-rong³, LIU De-yu³, WANG Ying-qun³, LI Xun², HU Chuan-huo²

1. Guangxi Key Laboratory of Livestock Genetic Improvement, Guangxi Institute of Animal Sciences, Nanning 530001, China;
2. College of Animal Science and Technology, Guangxi University, Nanning 530005, China;
3. Guangxi Work Station of Livestock ＆ Poultry Breed Improvement, Nanning 530001, China

Received:2014-02-07 Online:2014-09-20 Published:2014-09-24

摘要/Abstract

摘要： 为了评价黄芪多糖（Astragalus polysaccharides）对外来公猪精液冷冻保存的影响情况，为猪精液冷冻稀释液配方的改良提供理论依据，试验采集美系长白、大约克与杜洛克3种公猪的精液，用添加不同浓度（0、0.01%、0.02%、0.03%、0.04%、0.05%、0.06%）黄芪多糖的冷冻稀释液稀释，0.25 mL塑料细管分装并冷冻，测定解冻后精子活力、畸形率及顶体完整率，并进行相同浓度3种公猪之间和同种公猪不同浓度之间的比较。结果表明，在相同黄芪多糖浓度下，仅杜洛克公猪冻后精子顶体完整率在不添加黄芪多糖时显著高于大约克公猪（P<0.05），其余均无显著差异（P>0.05）；每种公猪的最佳黄芪多糖添加浓度均为0.04%，在该浓度下精液冻后质量均显著优于对照组（P<0.05）；各种公猪最佳黄芪多糖添加浓度下的精液冻后质量指标之间均无显著差异（P>0.05）。总之，稀释液中添加0.04%黄芪多糖在长白、大约克与杜洛克3种公猪的精液冷冻都可以取得较好的效果。

关键词: 猪; 精液; 冷冻保存; 黄芪多糖

Abstract: The objective of the present study was to assess the effects of Astragalus polysaccharides on cryopreservation of semen collected from adventive boars and provide a theorectical reference to improve the formula of diluents for boar semen cryopreservation. Semen was collected from three kinds of American-derived boars (Landrace, Large Yorkshire, Duroc) and diluted in solution supplimented with different concentrations of Astragalus polysaccharides (0, 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.06%). After being loaded into 0.25 mL straws the extended semen was freezed. Subsequently the qualitative parameters (motility, abnormality and acrosomal integrity) of frozen-thawed spermatozoa were estimated, and comparisons of mean values among three breeds of boar at the same conoentration or among treatments at the same breed of boar were performed. The results showed that,when compared without Astragalus polysaccharides acrosomal integrity of frozen-thawed Duroc boar spermatozoa was significantly higher than that of frozen-thawed Large Yorkshire boar spermatozoa (P<0.05), but no significant differences were observed when other comparisions were carried out at the same concentration (P>0.05). Of all the three breeds of boar, 0.04% was the optimal concentration of Astragalus polysaccharides supplied to semen diluents, at which the quality of frozen-thawed semen was significantly better than that of the control (P<0.05). There weren't significant differences in the quality of frozen-thawed semen among the three breeds of boar at the optimal concentration of added Astragalus polysaccharides (P>0.05). In brief, the extender supplimented with 0.04% Astragalus polysaccharides can demonstrate desirable parameters of frozen-thawed spermatozoa in all the three breeds of boar.

Key words: boar; semen; cryopreservation; Astragalus polysaccharides

中图分类号:

S814

杨尚雪, 潘天彪, 黄明光, 许春荣, 刘德玉, 王英群, 李珣, 胡传活. 黄芪多糖对猪精液冷冻保存效果的影响[J]. 中国畜牧兽医, 2014, 41(9): 179-183.

YANG Shang-xue, PAN Tian-biao, HUANG Ming-guang, XU Chun-rong, LIU De-yu, WANG Ying-qun, LI Xun, HU Chuan-huo. Effects of Astragalus Polysaccharides on the Quality of Frozen-thawed Boar Semen[J]. , 2014, 41(9): 179-183.

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