霉菌毒素对蛋鸡的危害及其残留与检测方法的研究进展

摘要/Abstract

摘要： 霉菌毒素对蛋鸡饲料的污染，不仅影响到蛋鸡健康，其在组织和鸡蛋中的残留也给食品安全带来很大隐患。作者就霉菌毒素的污染情况、对蛋鸡的影响、在肌肉、肝脏和肾脏等组织和鸡蛋中的残留情况及其检测方法的研究现状进行综述，从而为深入研究霉菌毒素提供一定的依据。

关键词:

霉菌毒素; 蛋鸡; 残留; 检测方法

Abstract: Feed mycotoxins contamination in laying hens diets posed severe threat to both laying hens health and animal products safety. In this review，we concluded the effects of mycotoxins in laying hens，residual in hens tissues and eggs，as well as the detection method to promote the research on mycotoxins.

Key words: mycotoxins; laying hens; residual; detection method

方思敏，柏凡，柏雪，郭春华，张正帆，彭忠利. 霉菌毒素对蛋鸡的危害及其残留与检测方法的研究进展[J]. 中国畜牧兽医.

FANG Si-min，BAI Fan，BAI Xue，GUO Chun-hua，ZHANG Zheng-fan，PENG Zhong-li. Research Progress on Detection Method，Hazard and Residual of Mycotoxins in Laying Hens[J]. .

参考文献

1 张秀竹.饲料中黄曲霉毒素B₁监测及其对蛋鸡肝脏脂类代谢和抗氧化功能的影响［D］.泰安：山东农业大学，2012.
2 敖志刚，陈代文.2006～2007年中国饲料及饲料原料霉菌毒素污染调查报告［J］.中国畜牧兽医，2008，35(1)：152～156.
3 Al-Anati L，Petzinger E.Immunotoxic activity of ochratoxin A［J］.Journal of Veterinary Pharmacology and Therapeutics，2006，29(2)：79～90.
4 Anastassiades M，Lehotay S J，Stajnbaher D，et al.Fast and easy multiresidue method employing acetonitrile extraction/partitioning and dispersive solid-phase extraction for the determination of pesticide residues in produce［J］.Journal of AOAC International，2003，86(2)：412～431.
5 Avantaggiato G，Havenaar R，Visconti A.Assessing the zearalenone-binding activity of adsorbent materials during passage through a dynamic in vitro gastrointestinal model［J］.Food and Chemical Toxicology，2003，41(10)：1283～1290.
6 Awad W A，Bohm J，Razzazi-Fazeli E，et al.Effects of deoxynivalenol on general performance and electrophysiological properties of intestinal mucosa of broiler chickens［J］.Poultry Science，2004，83(12)：1964～1972.
7 Awad W A，Rehman H，Bohm J，et al.Effects of luminal deoxynivalenol and L-proline on electrophysiological parameters in the jejunums of laying hens［J］.Poultry Science，2005a，84(6)：928～932.
8 Awad W A，Bohm J，Razzazi-Fazeli E，et al.In vitro effects of deoxynivalenol on electrical properties of intestinal mucosa of laying hens［J］.Poultry Science，2005b，84(6)：921～927.
9 Awad W A，Ghareeb K，Bohm J，et al.The impact of the Fusarium toxin deoxynivalenol (DON) on poultry［J］.International Journal of Poultry Science，2008，7(9)：827～842.
10 Biro K，Solti L，Vetro I B，et al.Tissue distribution of ochratoxin A as determined by HPLC and ELISA and histopathological effects in chickens［J］.Avian Pathology，2002，31(2)：141～148.
11 Chen C，Pearson A M，Coleman T H，et al.Tissue deposition and clearance of aflatoxins from broiler chickens fed a contaminated diet［J］.Food and Chemical Toxicology，1984，22(6)：447～451.
12 Chowdhury S R，Smith T K，Boermans H J，et al.Effects of feed-borne Fusarium mycotoxins on hematology and immunology of laying hens［J］.Poultry Science，2005，84(12)：1841～1850.
13 Cunha S C，Fernandes J O.Development and validation of a method based on a QuEChERS procedure and heart-cutting GC-MS for determination of five mycotoxins in cereal products［J］.Journal of Separation Science，2010，33(4～5)：600～609.
14 Del Bianchi M，Oliveira C A，Albuquerque R，et al.Effects of prolonged oral administration of aflatoxin B₁ and fumonisin B₁ in broiler chickens［J］.Poultry Science，2005，84(12)：1835～1840.
15 Denli M，Blandon J C，Guynot M E，et al.Efficacy of a new ochratoxin-binding agent (OcraTox) to counteract the deleterious effects of ochratoxin A in laying hens［J］.Poultry Science，2008，87(11)：2266～2272.
16 Desmarchelier A，Oberson J M，Tella P，et al.Development and comparison of two multiresidue methods for the analysis of 17 mycotoxins in cereals by liquid chromatography electrospray ionization tandem mass spectrometry［J］.Journal of Agricultural and Food Chemistry，2010，58(13)：7510～7519.
17 Elaroussi M A，Mohamed F R，El-Barkouky E M，et al.Experimental ochratoxicosis in broiler chickens［J］.Avian Pathology，2006，35(4)：263～269.
18 Frenich A G，Romero-Gonzalez R，Gomez-Perez M L，et al.Multi-mycotoxin analysis in eggs using a QuEChERS-based extraction procedure and ultra-high-pressure liquid chromatography coupled to triple quadrupole mass spectrometry［J］.Journal of Chromatography A，2011，1218(28)：4349～4356.
19 Hanif N Q，Naseem M，Khatoon S，et al.Prevalence of mycotoxins in poultry rations［J］.Pakistan Journal of Scientific and Industrial Research，2006，49(2)：120～124.
20 Hassan Z U，Khan M Z，Khan A，et al.Effects of individual and combined administration of ochratoxin A and aflatoxin B₁ in tissues and eggs of White Leghorn breeder hens［J］.Journal of the Science of Food and Agriculture，2012，92(7)：1540～1544.
21 Herebian D，Zuhlke S，Lamshot M，et al.Multi-mycotoxin analysis in complex biological matrices using LC-ESI/MS：Experimental study using triple stage quadrupole and LTQ-Orbitrap［J］.Journal of Separation Science，2009，32(7)：939～948.
22 Jestoi M，Rokka M，Jarvenpaa E，et al.Determination of Fusarium mycotoxins beauvericin and enniatins (A，A₁，B，B₁) in eggs of laying hens using liquid chromatography-tandem mass spectrometry (LC-MS/MS)［J］.Food Chemistry，2009，115(3)：1120～1127.
23 Jouany J P.Methods for preventing，decontaminating and minimizing the toxicity of mycotoxins in feeds［J］.Animal Feed Science and Technology，2007，137(3～4)：342～362.
24 Kilburn K H.Towards healthy homes［J］.Toxicology and Industrial Health，2009，25(9～10)：737～740.
25 Koesukwiwat U，Lehotay S J，Miao S，et al.High throughput analysis of 150 pesticides in fruits and vegetables using QuEChERS and low-pressure gas chromatography-time-of-flight mass spectrometry［J］.Journal of Chromatography A，2010，1217(43)：6692～6703.
26 Kppen R，Koch M，Siegel D，et al.Determination of mycotoxins in foods：Current state of analytical methods and limitations［J］.Applied Microbiology and Biotechnology，2010，86(6)：1595～1612.
27 Koynarski V，Stoev S，Grozeva N，et al.Experimental coccidiosis provoked by Eimeriaacervulina in chicks simultaneously fed on ochratoxin A contaminated diet［J］.Research in Veterinary Science，2007，82(2)：225～231.
28 Mortier L，Daeseleire E，Van Peteghem C.Liquid chromatographic tandem mass spectrometric determination of five coccidiostats in poultry eggs and feed［J］.Journal of Chromatography B， 2005，820(2)：261～270.
29 Niemiec J，Borzemska W，Golinski P，et al.The effect of ochratoxin A on egg quality， development of embryos and the level of toxin in eggs and tissues of hens and chicks［J］.Journal of Animal and Feed Sciences，1994，3(4)：309～316.
30 Placinta C M，D′Mello J P F，Macdonald A M C.A review of worldwide contamination of cereal grains and animal feed with Fusarium mycotoxins［J］.Animal Feed Science and Technology，1999，78(1～2)：21～37.
31 Ringot D，Chango A，Schneider Y J，et al.Toxicokinetics and toxicodynamics of ochratoxin A， an update［J］.Chemico-biological Interactions，2006，159(1)：18～46.
32 Rodrigues I，Naehrer K.A three-year survey on the worldwide occurrence of mycotoxins in feedstuffs and feed［J］.Toxins，2012，4(9)：663～675.
33 Sakhare P S，Harne S D，Kalorey D R，et al.Effect of toxiroak polyherbal feed supplement during induced aflatoxicosis，ochratoxicosis and combined mycotoxicoses in broilers［J］.Veterinarski Arhiv，2007，77(2)：129～146.
34 Saleemi M K，Khan M Z，Khan A，et al.Mycoflora of poultry feeds and mycotoxins producing potential of Aspergillus species［J］.Pakistan Journal of Botany，2010，42(1)：427～434.
35 Salem N M，Ahmad R.Mycotoxins in food from Jordan：Reliminary survey［J］.Food Control，2010，21(8)：1099～1103.
36 Shaafsma A W，Limay-Rios V，Paul D E，et al.Mycotoxins in fuel ethanol co-products derived from maize：A mass balance for deoxynivalenol［J］.Journal of the Science of Food and Agriculture，2009，89(9)：1574～1580.
37 Streit E，Schwab C，Sulyok M，et al.Multi-mycotoxin screening reveals the occurrence of 139 different secondary metabolites in feed and feed ingredients［J］.Toxins，2013，5(3)：504～523.
38 Sypecka Z，Kelly M，Brereton P.Deoxynivalenol and zearalenone residues in eggs of laying hens fed with a naturally contaminated diet：Effects on egg production and estimation of transmission rates from feed to eggs［J］.Journal of Agricultural and Food Chemistry，2004，52(17)：5463～5471.
39 Tangni E K，Waegeneers N，Van Overmeire I，et al.Mycotoxin analyses in some home produced eggs in belgium reveal small contribution to the total daily intake［J］.Science of the Total Environment，2009，407(15)：4411～4418.
40 Trucksess M W，Stoloff L，Young K，et al.Aflatoxicol and aflatoxins B₁ and M₁ in eggs and tissues of laying hens consuming aflatoxin-contaminated feed［J］.Poultry Science，1983，62(11)：2176～2182.
41 Verma J，Johri T S，Swain B K.Effect of varying levels of aflatoxin，ochratoxin and their combinations on the performance and egg quality characteristics in laying hens［J］.Asian Australasian Journal of Animal Sciences，2003，16(7)：1015～1019.
42 Westermann J，Pabst R.Lymphocyte subsets in the blood：Adiagnostic window on the lymphoid system［J］.Immunol Today，1990，11(11)：406～410.
43 Whelan M，Kinsella B，Furey A，et al.Determination of anthelmintic drug residues in milk using ultra high performance liquid chromatography-tandem mass spectrometry with rapid polarity switching［J］.Journal of Chromatography A，2010，1217(27)：4612～4622.

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