分子印迹传感器在兽药残留分析领域研究进展

doi:10.16431/j.cnki.1671-7236.2024.01.043

摘要/Abstract

摘要： 动物源食品中兽药、微生物毒素、防腐剂以及食品加工过程中的药物残留可引起明显的毒性作用和过敏反应，给人类健康带来威胁。分子印迹技术主要通过诱导模板在聚合物中形成特定识别位点，具有预定性、识别性以及实用性等优点，在兽药残留检测领域发挥了重要作用。使用分子印迹技术制备的聚合物受体所具有的坚固性、高亲和力、特异性和生产成本低等特性，使它们成为天然受体以及有毒化合物可选择的替代品。将分子印迹技术与传感器技术相结合，使两者的优势特点充分发挥，聚合物科学和纳米技术的进步有助于提高分子印迹聚合物传感器的性能，分子印迹传感器所适用的领域也进一步扩大。近年来许多高质量文献报道了分子印迹聚合物传感器在生物分子、滥用药物和爆炸物等领域的研究，推动了该技术在生命科学和危化品领域的应用。作者重点简述了分子印迹技术与分子印迹传感器的原理，介绍了基于分子印迹聚合物的传感器技术的最新成就以及面向兽药残留检测应用领域的研究。

关键词: 分子印迹; 分子印迹聚合物; 分子印迹传感器; 兽药残留

Abstract: Animal derived foods containing veterinary drugs, microbial toxins, preservatives, and drug residues during food processing can cause significant toxic effects and allergic reactions, posing a threat to human health.Molecular imprinting technology mainly induces templates to form specific recognition sites in polymers, which has advantages such as predictability, recognition, and practicality, and has played an important role in the field of veterinary drug residue detection.The robustness, high affinity, specificity, and low production cost of polymer receptors prepared using molecular imprinting technology make them an alternative to natural receptors and toxic compounds.By combining molecular imprinting technology with sensor technology, the advantages and characteristics of both can be fully utilized.The progress of polymer science and nanotechnology can help improve the performance of molecular imprinted polymer sensors, and the applicable fields of molecular imprinted sensors are further expanded.In recent years, many high-quality literature has reported on the research of molecularly imprinted polymer sensors in the fields of biomolecules, abused drugs, and explosives, promoting the application of this technology in the fields of life sciences and hazardous chemicals.The author focuses on the principles of molecular imprinting technology and molecular imprinting sensors, and introduces the latest achievements of sensor technology based on molecular imprinted polymers, as well as research in the field of veterinary drug residue detection applications.

Key words: molecular imprinting; molecularly imprinted polymer; molecular imprinting sensor; veterinary drug residue

中图分类号:

S859.1

玄艺, 张宇, 黄晓宁, 姜昕妤, 魏可欣, 王晓通, 谢晓程, 李臣贵. 分子印迹传感器在兽药残留分析领域研究进展[J]. 中国畜牧兽医, 2024, 51(1): 427-433.

XUAN Yi, ZHANG Yu, HUANG Xiaoning, JIANG Xinyu, WEI Kexin, WANG Xiaotong, XIE Xiaocheng, LI Chengui. Research Progress of Molecular Imprinting Sensors in the Field of Veterinary Drug Residue Analysis[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 427-433.

参考文献

[1] 王恩辉, 张志然.我国兽药残留检测标准现状[J].品牌与标准化, 2023, 381(4):23-26. WANG E H, ZHANG Z R.Current status of veterinary drug residue detection standards in China[J].Brand and Standardization, 2023, 381(4):23-26.(in Chinese)
[2] 黎晓林.钦州市动物产品中兽药残留现状调查分析及安全管理对策[D].南宁:广西大学, 2020. LI X L.Investigation and analysis of veterinary drug residues in animal products in Qinzhou city and safety management countermeasures[D].Nanning:Guangxi University, 2020.(in Chinese)
[3] 高连仁.F市畜产品质量安全监督管理问题研究[D].沈阳:东北大学, 2017. GAO L R.Research on the quality and safety supervision of animal products in F city[D].Shenyang:Northeastern University, 2017.(in Chinese)
[4] 李杉, 付鹏钰, 马青青, 等.河南省市售畜肉兽药和违禁药物残留暴露风险评估[J].预防医学, 2021, 33(1):81-83. LI S, FU P Y, MA Q Q, et al.Risk assessment of veterinary drug and banned drug residues in meat from market in Henan province[J].Preventive Medicine, 2021, 33(1):81-83.(in Chinese)
[5] 郭添荣.动物源食品中兽药残留的高通量筛查方法研究[D].成都:成都大学, 2021. GUO T R.Study on high-throughput screening methods for veterinary drug residues in foods of animal origin[D].Chengdu:Chengdu University, 2021.(in Chinese)
[6] POLYAKOV M V.Adsorption properties and structure of silica gel[J].Zhurnal Fizicheskoi Khimii, 1993, 2:799-805.
[7] 田浩, 李子木, 汪长征, 等.分子印迹荧光传感构建及应用[J].化学进展, 2022, 34(3):593-608. TIAN H, LI Z M, WANG C Z, et al.Construction and application of molecular imprinted fluorescence sensor[J].Chemical Progress, 2022, 34(3):593-608.(in Chinese)
[8] ALI D, KHALED Y, SHIVA A, et al.Molecularly imprinted polymer (MIP) based core-shell microspheres for bacteria isolation[J].Polymer, 2022, 251:124917.
[9] 王强, 杜洪振, 韩格, 等.分子印迹传感器在肉品安全检测中的应用进展[J].食品科学, 2022, 43(9):346-353. WANG Q, DU H Z, HAN G, et al.Adevances in the application of molecular imprinting polymer-based sensors for meat safety detection[J]. Food Science, 2022, 43(9):346-353.(in Chinese)
[10] LOKMAN U, ANTHONY P F T.Molecularly-imprinted polymer sensors:Realising their potential[J].Biosensors and Bioelectronics, 2016, 76:131-144.
[11] PILETSKY S A, BUTOVICH I A, KUKHAR V P.Design of molecular sensors on the basis of substrate-selective polymer membranes[J].Zhurnal Analiticheskoi Khimii, 1992, 47:1681-1684.
[12] PILETSKY S A, PILETSKAYA E V, PANASYUK T L, et al.Imprinted membranes for sensor technology:Opposite behavior of covalently and noncovalentlyimprinted membranes[J].Macromolecules, 1998, 31, 2137-2140.
[13] CHEN L X, WANG X Y, LU W H, et al.Molecular imprinting:Perspectives and applications[J].Chemical Society Reviews, 2016, 45(8):2137-2211.
[14] HULANICKI A, GLAB S, INGMAN F.Chemical sensors:Definitions and classification[J].Pure and Applied Chemistry, 1991, 63:1247-1250.
[15] EKOMO M V, BRANGER C, GAVRILA A, et al.Electrochemical molecularly imprinted polymers in microelectrode devices[J].MRS Communications, 2020, 10(2):324-331.
[16] 胡文英, 刘涛, 吴强强.苯酚分子印迹电化学传感器的制备及应用[J].高分子通报, 2022, 12:129-137. HU W Y, LIU T, WU Q Q.Preparation and application of phenol electrochemical sensor based on molecularly imprinted polymer[J].Polymer Bulletin, 2022, 12:129-137.(in Chinese)
[17] TORKASHVAND M, GHOLIVAND M B, MALEKZADE G H.Construction of a new electrochem-ical sensor based on molecular imprinting recognition sites on multiwall carbon nanotube surface for analysis of ceftazidime in real samples[J].Sensors and Actuators B-Chemical, 2016, 231:759-767.
[18] YUN Y G, PAN M F, FANG G Z, et al.Molecularly imprinted electrodeposition O-aminothiophenol sensor for selective and sensitive determination of amantadine in animal-derived foods[J].Sensors and Actuators B-Chemical, 2017, 238:32-39.
[19] WANG H W, YAO S, LIU Y Q, et al.Molecularly imprinted electrochemical sensor based on au nanoparticles in carboxylated multi-walled carbon nanotubes for sensitive determination of olaquindox in food and feedstuffs[J].Biosensors and Bioelectronics, 2017, 87:417-421.
[20] WEI G, FU W, ZHANG H X, et al.A novel molecularly imprinted electrochemical sensor modified with carbon dots, chitosan, gold nanoparticles for the determination of patulin[J].Biosensors and Bioelectronics, 2017, 98:299-304.
[21] ROUSHANI M, NEZHADALI A, JALILIAN Z, et al.Development of novel dlectrochemical sensor on the base of molecular imprinted polymer decorated on SiC nanoparticles modified glassy carbon electrode for selective determination of loratadine[J].Materials Science & Engineering C-Materials for Biological Applications, 2017, 71:1106-1114.
[22] 丁同英, 袁航.分子印迹传感器在真菌毒素检测中的应用研究进展[J].食品与机械, 2021, 37(12):197-201. DING T Y, YUAN H.Application and progress of molecularly imprinted sensors in mycotoxins detection[J].Food and Machinery, 2021, 37(12):197-201.(in Chinese)
[23] BAHARI D, BABAMIRI B, SALIMI A.Ultrasensitive molecularly imprinted fluorescence sensor for simultaneous determination of CA125 and CA15-3 in human serum and OVCAR-3 and MCF-7 cells lines using Cd and Ni nanoclusters as new emitters[J].Analytical and Bioanalytical Chemistry, 2021, 413(15):4049-4061.
[24] JANINA K, HARALD K, BURGHARDT W, et al.Novel optical nanosensors for probing and imaging live cells[J].Nanomedicine:Nanotechnology, Biology and Medicine, 2010, 6:214-226.
[25] ZHONG Z, LI J H, WANG X Y, et al.Quantum dots based mesoporous structured imprinting microspheres for the sensitive fluorescent detection of phycocyanin[J].ACS Applied Materials and Interfaces, 2015, 17:9118-9127.
[26] XU J J, ZHANG R R, LIU C X, et al.Highly selective electrochemiluminescence sensor based on molecularly imprinted-quantum dots for the sensitive detection of cyfluthrin[J].Sensors, 2020, 20(3):884.
[27] KHAN Z G, PATIL P O.A comprehensive review on carbon dots and graphene quantum dots based fluorescent sensor for biothiols[J].Microchemical Journal, 2020, 157:105011.
[28] 张慧琴, 孙立勋, 杨艺哲, 等.一种基于功能化修饰磁性纳米颗粒的石墨烯基光学生物传感器[J].光学学报, 2020, 40(11):162-169. ZHANG H Q, SUN L X, YANG Y Z, et al.Graphene-based optical biosensor using functionalized magnetic nanoparticles[J].Acta Optica Sinica, 2020, 40(11):162-169.(in Chinese)
[29] MEHRZAD-SAMARIN M, FARIDBOD F, DEZFULI S A, et al.A novel metronidazole fluorescent nanosensor based on graphene quantum dots embedded silica molecularly imprinted polymer[J].Biosensors and Bioelectronics, 2017, 92:618-623.
[30] WITTAYA N, KANOKWAN C, KESSARIN N, et al.A highly selective fluorescent sensor for manganese(Ⅱ) ion detection based on N, S-doped carbon dots triggered by manganese oxide[J].Dyes and Pigments, 2022, 203:110325.
[31] HAO T F, WEI X, NIE Y, et al.An eco-friendly molecularly imprinted fluorescence composite material based on carbon dots for fluorescent detection of 4-nitrophenol[J].Microchimica Acta, 2016, 183(7):2197-2203.
[32] CUI Y X, SU A X, FENG J Y, et al.Development of silica molecularly imprinted polymer on carbon dots as a fluorescence probe for selective and sensitive determination of cetirizine in saliva and urine[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy, 2022, 264:120293.
[33] LARISSA F S, MARTINS M C, GALVÃO R L D.Simple and cheap preparation of fluorescence paper sensor based in carbon dot for visual detection of chloramphenicol.[J].Luminescence:The Journal of Biological and Chemical Luminescence, 2022, 38(7):1319-1329.
[34] 李婧雯, 戢凯伦, 陈苗苗, 等.ZIF-8/SQDs电化学发光传感器超灵敏检测miRNA-141研究[J].湖北大学学报(自然科学版), 2022, 44(5):547-554. LI J W, JI K L, CHEN M M, et al.Construction of ZIF-8/SQDs-based electrochemiluminescence sensing platform for ultrasensitive detection of miRNA-141[J].Journal of Hubei University (Natural Science Edition), 2022, 44(5):547-554.(in Chinese)
[35] 戢凯伦, 徐玮, 王颖, 等.基于RuSi@PEI纳米粒子构建分子印迹电化学发光传感器检测普萘洛尔[J].分析科学学报, 2020, 36(5):747-752. JI K L, XU W, WANG Y, et al.RuSi@PEI nanoparticles-based molecularly imprinted electrochemiluminescence sensors for propranolol detection[J].Journal of Analytical Sciences, 2020, 36(5):747-752.(in Chinese)
[36] 马慧颖, 卢邦荣, 燕昱霖, 等.量子点表面分子印迹光纤传感器的制备及应用[J].激光生物学报, 2019, 28(6):523-528. MA H Y, LU B R, YAN Y L, et al.Preparation and application of optical fiber sensors based on quantum dots surface aolecularly imprinting polymers[J].Journal of Laser Biology, 2019, 28(6):523-528.(in Chinese)
[37] RAZANSKY D, EINZIGER P D, ADAM D R.Cavity-enhanced biosensing utilizing plasmon resonance modes[J].Conference Proceedings, 2006, 1:4602-4605.
[38] JON A, YUNUS S, ROBERTA D, et al.Synthesis of molecularly imprinted polymer nanoparticles for α-casein detection using surface plasmon resonance as a milk allergen sensor[J].ACS Sensors, 2018, 3(2):418-424.
[39] 戴正强.恩诺沙星分子印迹聚合物压电传感器的构建[D].长春:吉林农业大学, 2016. DAI Z Q.Construction of enrofloxacin molecularly imprinted polymer piezoelectric sensors[D].Changchun:Jilin Agricultural University, 2016.(in Chinese)
[40] 王光宇.苯巴比妥分子印迹压电传感器的构建及在动物源性食品检测中的应用[D].长春:吉林农业大学, 2019. WANG G Y.Construction of phenobarbital molecularly imprinted piezoelectric sensor and its application in the detection of animal derived food[D].Changchun:Jilin Agricultural University, 2019.(in Chinese)
[41] FAO.Enhancing Early Warning Capabilities and Capacities for Food Safety[M].Rome:FAO, 2015.
[42] 林志达, 黄晶, 蔡杰, 等.兽药残留种类及检测方法[J].中国动物保健, 2022, 24(1):1. LIN Z D, HUANG J, CAI J, et al.Types and detection methods of veterinary drug residues[J].China Animal Health, 2022, 24(1):1.(in Chinese)
[43] 陈羚, 谢海洋.生物传感器在食品安全检测中的应用[J].食品安全导刊, 2021, 28:137-138. CHEN L, XIE H Y.Application of biosensors in food safety testing[J].Food Safety Journal, 2021, 28:137-138.(in Chinese)
[44] YAQOOB A A, PARVEEN T, UMAR K, et al.Role of nanomaterials in the treatment of wastewater:A review[J].Water, 2020, 12(2):495.
[45] CABALLERO B, TRUGO L, FINGLAS P M.Encyclopedia of Food Science and Nutrition[M].New York:Academic Press An imprint of Elsevier Science, 2003.
[46] TONGQIANG J, TIANQI L, WEI D, et al.Prediction of safety risk levels of veterinary drug residues in freshwater products in China based on transformer[J].Foods, 2022, 11(12):1690.
[47] 丁同英, 袁航.分子印迹传感器在真菌毒素检测中的应用研究进展[J].食品与机械, 2021, 37(12):197-201. DING T Y, YUAN H.Application and progress of molecularly imprinted sensors in mycotoxins detection[J].Food and Machinery, 2021, 37(12):197-201.(in Chinese)