奶及奶制品中兽药残留高通量检测方法研究进展

doi:10.16431/j.cnki.1671-7236.2020.10.041

摘要/Abstract

摘要： 在生产中兽药的滥用可能导致牛奶中的兽药残留并对消费者健康造成风险。由于兽药种类较多，单一检测效率较低，而高通量检测技术可以一次性检测多种兽药残留，省时省力，因此成为当前研究的热点。在样品前处理方面，分散固相萃取技术（如QuEChERS方法）由于其简单、快速、成本低等特点，广泛应用于样品净化过程；另一方面，超高效液相色谱串联质谱由于其分离快、灵敏度与准确度高等特点，展示出比高效液相色谱法更好的分析能力和应用前景。随着新技术的应用和新仪器的使用，兽药残留检测在前处理效率、仪器灵敏度和高通量检测方面发展迅速。作者结合近些年发表的文献，综述了奶及奶制品中兽药残留前处理技术（液液萃取法、固相萃取法、QuEChERS和盐析支持液液萃取）和高通量检测技术（高效液相色谱法、液相色谱-质谱联用、超高效液相色谱串联质谱和液相色谱-飞行时间质谱法），为今后相关检测研究提供参考。

关键词: 兽药残留; 牛奶; 高通量

Abstract: The abuse of veterinary drugs in production may lead to veterinary drug residues in milk and pose a risk to consumer health.As the kinds of veterinary drugs are too many,the detection method for single target presents low efficiency.Whereas the high-throughput detection technology can detect various veterinary drug residues simultaneously,with time and labor saving,and become a research hot spot.In the aspect of sample pretreatment,dispersed solid phase extraction techniques,such as QuEChERS method,are widely used in sample purification due to its features of simplicity,rapidity and low cost.On the other hand,because of its characteristics of fast separation,high sensitivity and accuracy,ultra-high performance liquid chromatography (UPLC) tandem mass spectrometry (MS) has shown better analytical performance and application prospect than high performance liquid chromatography (HPLC).With the application of new technologies and instruments,veterinary drug residue detection has developed rapidly in terms of pretreatment efficiency,instrument sensitivity and high-throughput detection.In this paper,based on recent literatures and publications,the pretreatment techniques and high-throughput detection technology of veterinary drug residues in milk and dairy products were reviewed,to provide a reference for future research.The pretreatment techniques introduced liquid-liquid extraction (LLE),solid phase extraction (SPE),QuEChERS and salting out support liquid extraction,while the high-throughput detection technology included high-performance liquid chromatography (HPLC),liquid chromatography-mass spectrometry (LC-MS),ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS) and liquid chromatography-time of flight mass spectrometry (LC-TOF-MS).

Key words: veterinary drug residues; milk; high-throughput

中图分类号:

S879.1

罗润博, 张养东, 郑楠, 王加启. 奶及奶制品中兽药残留高通量检测方法研究进展[J]. 中国畜牧兽医, 2020, 47(10): 3410-3416.

LUO Runbo, ZHANG Yangdong, ZHENG Nan, WANG Jiaqi. Advances in High-throughput Detection of Veterinary Drug Residues in Milk and Dairy Products[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3410-3416.

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