鸭极低密度脂蛋白受体和载脂蛋白E受体2在肌肉组织中的发育性表达模式研究

摘要/Abstract

摘要： 为探索极低密度脂蛋白受体(very low density lipoprotein receptor，VLDLR)和载脂蛋白E受体2(apolipoprotein E receptor 2，ApoER2)在鸭肌内脂肪性状发育中的作用机制，本研究应用实时荧光定量PCR检测了VLDLR和ApoER2基因在1~8周龄北京鸭胸肌、腿肌、心肌中的表达丰度，分析其在早期生长阶段的发育性变化。结果显示VLDLR和ApoER2在鸭不同肌肉中的表达具有组织特异性。VLDLR 基因mRNA丰度在3种肌肉组织中变化较大，且明显呈现上升—下降（胸肌）、下降—上升—下降—上升（腿肌）及下降—上升—下降（心肌）3种模式的变化；ApoER2基因mRNA表达量在胸肌第3周龄时达最高（P<0.01），在腿肌组织中前6周保持相对较低水平，在后2周迅速上升，且显著高于其余各周（P<0.01），而在心肌组织中呈上升—下降趋势，且在前2周表达量显著高于其余各周（P<0.01）。同周龄VLDLR和ApoER2基因mRNA的表达量在不同部位肌肉中表现出明显差异。以上结果说明，VLDLR和ApoER2基因在鸭肌内脂肪性状形成中起到了重要作用，且存在着时空特异性。

关键词: 鸭; 极低密度脂蛋白受体(VLDLR); 载脂蛋白E受体2(ApoER2); 表达模式

Abstract: To identify the possible role of very low density lipoprotein receptor (VLDLR) and apolipoprotein E receptor 2 (ApoER2) genes in duck muscles, the developmental changes of VLDLR and ApoER2 mRNA level in breast muscle, leg muscle and cardiac muscle during duck early growth stages were investigated using Real-time fluorescent quantity PCR. The results showed that VLDLR and ApoER2 mRNA expression level was different in different muscle tissues from 1 to 8 week in duck and had tissue-dependent. Three expression patterns of VLDLR mRNA during growth were found: Fluctuating expression as up-down (breast muscle), as down-up-down-up (leg muscle) and as down-up-down (cardiac muscle). For ApoER2 mRNA expression level, in breast muscle, the mRNA expression level was the highest (P<0.01) in 3 week old duck. In leg muscle, the mRNA expression level kept a low level in the first 6 weeks, then had a significant increase in 7 and 8 weeks, which was significantly higher than that of other weeks (P<0.01). During the cardiac tissue development, the mRNA expression level presented as up-down with growing, and had a higher expression level in the first 2 weeks (P<0.01). In the same week VLDLR and ApoER2 mRNA expression level had tissue-dependent in muscles of three different sites in duck. Our results indicated that VLDLR and ApoER2 genes could play an important role in intramuscular fat accumulation with spatial and temporal specificity during duck early growth stages.

Key words: duck; VLDLR; ApoER2; expression pattern

苟华，胡深强，潘志雄，刘贺贺，李亮，王继文. 鸭极低密度脂蛋白受体和载脂蛋白E受体2在肌肉组织中的发育性表达模式研究[J]. 中国畜牧兽医.

GOU Hua，HU Shen-qiang，PAN Zhi-xiong，LIU He-he，LI Liang，WANG Ji-wen. Developmental Expression Patterns of VLDLR and ApoER2 Genes in Duck Muscles[J]. .

参考文献

1 王健, 臧大存, 左伟勇,等. 番鸭、樱桃谷鸭及高邮鸭产肉性能及肉质特性研究［J］. 扬州大学学报, 2008, 29（3）: 72～76.
2 李慧芳, 王强, 厉宝林, 等. 日粮不同蛋白质水平对育成期高邮鸭生长性能及氮沉积率的影响［J］.中国畜牧兽医, 2009, 36(10): 8～11.
3 张军, 龚道清, 段修军, 等. 高邮鸭体脂沉积规律的研究［J］. 江苏农业研究, 2001, 22(4): 39～42.
4 佟建民.饲料配方手册［M］. 第2版. 北京：中国农业大学出版社, 2007.
5 Alvarenga R R, Zangeronimo M G, Pereira L J, et al. Lipoprotein metabolism in poultry. World’s Poultry Science Journal, 2011, 67：431.
6 Andersen O M, Yeung C H, Vorum H, et al. Essential role of the apolipoprotein E receptor-2 in sperm development. J Biol Chem, 2003, 278(26): 23989～23995.
7 Chartrin P, Bernadet M D, Guy G, et al. Do age and feeding levels have comparable effects on fat deposition in breast muscle of mule ducks. Animal, 2007, 1: 113～123.
8 Griffin H D, Windsor D, Whitehead C C. Changes in lipoprotein metabolism and body composition in chickens in response to divergent selection for plasma very low density lipoprotein concentration. Br Poult Sci, 1991, 32(1): 195～201.
9 Howell B W, Herz J. The LDL receptor gene family: Signaling functions during development. Curr Opin Neurobiol, 2001, 11(1): 74～81.
10 Iijima H, Miyazawa M, Sakai J, et al. Expression and characterization of a very low density lipoprotein receptor variant lacking the O-linked sugar region generated by alternative splicing. J Biochem, 1998, 124(4): 747～755.
11 Jeon H, Blacklow S C. Structure and physiologic function of the low-density lipoprotein receptor. Annu Rev Biochem, 2005, 74: 535～562.
12 Kim D H, Iijima H, Goto K, et al. Human apolipoprotein E receptor 2. A novel lipoprotein receptor of the low density lipoprotein receptor family predominantly expressed in brain. J Biol Chem, 1996, 271(14): 8373～8380.
13 Korschineck I, Ziegler S, Breuss J, et al. Identification of a novel exon in apolipoprotein E receptor 2 leading to alternatively spliced mRNAs found in cells of the vascular wall but not in neuronal tissue. J Biol Chem, 2001, 276(16): 13192～13197.
14 Livak K J, Schmittgen T D. Analysis of relative gene expression data using Real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods, 2001, 25(4): 402～408.
15 May P, Bock H H, Nimpf J et al. Differential glycosylation regulates processing of lipoprotein receptors by gamma-secretase. J Biol Chem, 2003, 278(39): 37386～37392.
16 Ndiaye K, Fayad T, Silversides D W, et al. Identification of downregulated messenger RNAs in bovine granulosa cells of dominant follicles following stimulation with human chorionic gonadotropin. Biol Reprod, 2005, 73(2): 324～333.
17 Perez-Garcia C G, Tissir F, Goffinet A M, et al. Reelin receptors in developing laminated brain structures of mouse and human. Eur J Neurosci, 2004, 20(10): 2827～2832.
18 Reddy S S, Connor T E, Weeber E J, et al. Similarities and differences in structure, expression, and functions of VLDLR and ApoER2. Mol Neurodegener, 2011, 6: 30.
19 Ruiz J A, Guerrero L, Arnau J, et al. Descriptive sensory analysis of meat from broilers fed diets containing vitamin E or beta-carotene as antioxidants and different supplemental fats. Poult Sci， 2001， 80(7): 976～982.
20 Ruusunen M，Puolanne E. Histochemical properties of fibre types in muscles of wild and domestic pigs and the effect of growth rate on muscle fibre properties. Meat Sci， 2004， 67(3): 533～539.
21 Ryu Y C， Kim B C. The relationship between muscle fiber characteristics, postmortem metabolic rate, and meat quality of pig longissimus dorsi muscle. Meat Sci， 2005， 71(2): 351～357.
22 Shen G Q, Li L, Girelli D, et al. An LRP8 variant is associated with familial and premature coronary artery disease and myocardial infarction. Am J Hum Genet, 2007, 81(4): 780～791.
23 Takahashi S, Sakai J, Fujino T, et al. The very low-density lipoprotein (VLDL) receptor: Characterization and functions as a peripheral lipoprotein receptor. J Atheroscler Thromb， 2004， 11(4): 200～208.
24 Tiebel O, Oka K, Robinson K, et al. Mouse very low-density lipoprotein receptor (VLDLR): Gene structure, tissue-specific expression and dietary and developmental regulation. Atherosclerosis, 1999, 145(2): 239～251.
25 Wang C, Li S J, Yu W H, et al. Cloning and expression profiling of the VLDLR gene associated with egg performance in duck (Anas platyrhynchos). Genet Sel Evol, 2011, 43: 29.
26 Webb J C, Patel D D, Jones M D, et al. Characterization and tissue-specific expression of the human‘very low density lipoprotein (VLDL) receptor’mRNA. Hum Mol Genet, 1994, 3(4): 531～537.
27 Willnow T E. The low-density lipoprotein receptor gene family: Multiple roles in lipid metabolism.J Mol Med (Berl), 1999, 77(3): 306～315.

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