猪繁殖与呼吸综合征病毒的危害及免疫策略

摘要/Abstract

摘要： 猪繁殖与呼吸综合征（porcine reproductive and respiratory syndrome，PRRS）一直是危害世界养猪业较严重的疾病，给世界养猪业造成巨大经济损失。其病毒感染机理、抗体依赖性增强(ADE)作用及高度变异性使目前的商品化疫苗存在着缺陷，不能完全阻止经呼吸系统及胎盘传播猪繁殖与呼吸综合征病毒（porcine reproductive and respiratory syndrome virus，PRRSV）。鉴于此，应结合PRRSV的致病特点、免疫特性及生物学特性探索性地设计新型疫苗，对预防该病具有重要意义。

关键词: 猪繁殖与呼吸综合征病毒; 危害; 免疫

Abstract: Porcine reproductive and respiratory syndrome is a serious disease, causing significantly economical impacts on the swine industry worldwide. Because of the mechanism of virus infection, the characteristics of antibody-dependent enhancement and a high degree of variability, the commercial vaccines currently exist defects that can’t completely prevent the respiratory system and placental transmission of porcine reproductive and respiratory syndrome virus （PRRSV）. Therefore, according to the pathogenic characteristics, PRRSV immune characteristics and biological characteristics, the new vaccines are designed which have important significances to the prevention of the disease.

Key words: porcine reproductive and respiratory syndrome virus; harm; immunization

王振华，曹晓涵，宋禾，王迪，曾宪垠. 猪繁殖与呼吸综合征病毒的危害及免疫策略[J]. 中国畜牧兽医.

WANG Zhen-hua, CAO Xiao-han, SONG He, WANG Di, ZENG Xian-yin. The Harm and Immunization Strategy of Porcine Reproductive and Respiratory Syndrome Virus[J]. .

参考文献

1 王娇，孙继国，陈赛娟，等. 猪繁殖与呼吸综合征病毒河北地方株ORF6基因的克隆及原核表达[J]. 中国动物检疫，2009，26(4)：34～36.
2 刘萍，陈苗苗，乔莉萍，等. 猪繁殖与呼吸综合征病毒致病机理的研究进展[J]. 中国畜牧兽医，2010，37(1)：158～160.
3 苏丹萍，王文豪，张显浩，等. 高致病性猪繁殖与呼吸综合征、猪圆环病毒病和副猪嗜血杆菌病混合感染的诊断与防制[J]. 中国畜牧兽医，2012，39(8)：186～189.
4 陈声，杨汉春. PRRS病毒M蛋白在大肠埃希氏菌中的高效表达[J]. 中国兽医科技，2001，31(5)：6～8.
5 周艳君，田志军，刘金霞，等. 猪繁殖与呼吸综合征病毒M蛋白B细胞抗原表位的鉴定[J]. 中国预防兽医学报，2009，31(4)：251～255.
6 郑其升，毕志香，李鹏，等. 共表达猪呼吸与繁殖障碍综合征病毒NJ-a株ORF.4、ORF5与ORF6基因重组改良型痘苗病毒安卡拉株的构建[J]. 微生物学报，2007，47(2)：345～349
7 姚敬明，孟帆，吴忻，等. 猪繁殖与呼吸综合征母源抗体和免疫抗体的消长规律研究[J]. 中国畜牧兽医，2010，37(4)：205～208.
8 胡守萍，张卓，蔡雪辉，等. 猪繁殖与呼吸综合征病毒弱毒疫苗和变异株灭活疫苗的免疫效果评价[J]. 中国预防兽医学报，2009(5)：392～396.
9 薛江东，马国文，袁宝，等. 猪繁殖与呼吸综合征病毒M蛋白基因疫苗的构建及在小鼠的免疫试验[J]. 内蒙古民族大学学报（自然科学版），2006，21(6)：649～652.
10 薛江东. 猪繁殖与呼吸综合征病毒M蛋白基因重组犬2型腺病毒活载体疫苗的构建及其免疫原性研究[D]. 通辽市：内蒙古民族大学，2007.
11 薛强，邹明强，童光志. 病毒感染的抗体依赖性增强作用及其机制[J]. 中国预防兽医学报，2008，30(9)：743～746.
12 Amonsin A, Kedkovid R, Puranaveja S, et al. Comparative analysis of complete nucleotide sequence of porcine reproductive and respiratory syndrome virus (PRRSV) isolates in Thailand (US and EU genotypes)[J]. Virol J, 2009, 6: 143.
13 Batista L, Pijoan C, Dee S, et al. Virological and immunological responses to porcine reproductive and respiratory syndrome virus in a large population of gilts[J]. Canadian Journal of Veterinary Research, 2004, 68(4): 267.
14 Blaha T. The “colorful” epidemiology of PRRS[J]. Veterinary Research, 2000, 31(1): 77～83.
15 Calzada-Nova G, Schnitzlein W, Husmann R, et al. Characterization of the cytokine and maturation responses of pure populations of porcine plasmacytoid dendritic cells to porcine viruses and toll-like receptor agonists[J]. Veterinary Immunology and Immunopathology, 2010, 135(1～2): 20～33.
16 Charerntantanakul W. Adjuvants for porcine reproductive and respiratory syndrome virus vaccines[J]. Veterinary Immunology and Immunopathology, 2009, 129(1～2): 1～13.
17 Cho J G, Dee S A. Porcine reproductive and respiratory syndrome virus[J]. Theriogenology, 2006, 66(3): 655～662.
18 Christopher-Hennings J, Nelson E A, Althouse G C, et al. Comparative antiviral and proviral factors in semen and vaccines for preventing viral dissemination from the male reproductive tract and semen[J]. Animal Health Research Reviews, 2008, 9(1): 59～70.
19 Diaz I, Darwich L, Pappaterra G, et al. Different European-type vaccines against porcine reproductive and respiratory syndrome virus have different immunological properties and confer different protection to pigs[J]. Virology, 2006, 351(2): 249～259.
20 Jeong H J, Song Y J, Lee S W, et al. Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome virus[J]. Clinical and Vaccine Immunology, 2010, 17(4): 503～512.
21 Kang I, Ha Y, Kim D, et al. Localization of porcine reproductive and respiratory syndrome virus in mammary glands of experimentally infected sows[J]. Research in Veterinary Science, 2010, 88(2): 304～306.
22 Kim W I, Yoon K J. Molecular assessment of the role of envelope-associated structural proteins in cross neutralization among different PRRS viruses[J]. Virus Genes, 2008, 37(3): 380～391.
23 Kimman T G, Cornelissen L A, Moormann R J, et al. Challenges for porcine reproductive and respiratory syndrome virus (PRRSV) vaccinology[J]. Vaccine, 2009, 27(28): 3704～3718.
24 Kitikoon P, Vincent A L, Jones K R, et al. Vaccine efficacy and immune response to swine influenza virus challenge in pigs infected with porcine reproductive and respiratory syndrome virus at the time of SIV vaccination[J]. Veterinary Microbiology, 2009, 139(3): 235～244.
25 Labarque G G, Nauwynck H J, Van Reeth K, et al. Effect of cellular changes and onset of humoral immunity on the replication of porcine reproductive and respiratory syndrome virus in the lungs of pigs[J]. Journal of General Virology, 2000, 81(5): 1327～1334.
26 Laohasittikul P, Boonarpha N, Pongprapachuen Y, et al. Antigen distribution of porcine reproductive and respiratory syndrome virus (PRRSV) in Thai crossbred pigs using immunohistochemistry[J]. Wetchasan Sattawaphaet, 2004, 34(3):39～48.
27 Li G, Jiang P, Li Y, et al. Inhibition of porcine reproductive and respiratory syndrome virus replication by adenovirus-mediated RNA interference both in porcine alveolar macrophages and swine[J]. Antiviral Research, 2009, 82(3): 157～165.
28 Li Y, Wang X, Bo K, et al. Emergence of a highly pathogenic porcine reproductive and respiratory syndrome virus in the Mid-Eastern region of China[J]. The Veterinary Journal, 2007, 174(3): 577～584.
29 Lopez O, Oliveira M, Garcia E A, et al. Protection against porcine reproductive and respiratory syndrome virus (PRRSV) infection through passive transfer of PRRSV-neutralizing antibodies is dose dependent[J]. Clinical and Vaccine Immunology, 2007, 14(3): 269～275.
30 Luo R, Xiao S, Jiang Y, et al. Porcine reproductive and respiratory syndrome virus (PRRSV) suppresses interferon-β production by interfering with the RIG-Ⅰ signaling pathway[J]. Molecular Immunology, 2008, 45(10): 2839～2846.
31 Mateu E, Diaz I. The challenge of PRRS immunology[J]. The Veterinary Journal, 2008, 177(3): 345～351.
32 Meng X. Heterogeneity of porcine reproductive and respiratory syndrome virus: Implications for current vaccine efficacy and future vaccine development[J]. Veterinary Microbiology, 2000, 74(4): 309～329.
33 Mortensen S, Stryhn H, Sgaard R, et al. Risk factors for infection of sow herds with porcine reproductive and respiratory syndrome (PRRS) virus[J]. Preventive Veterinary Medicine, 2002, 53(1): 83～101.
34 Murtaugh M P, Xiao Z, Zuckermann F. Immunological responses of swine to porcine reproductive and respiratory syndrome virus infection[J]. Viral Immunology, 2002, 15(4): 533～547.
35 Osorio F A, Galeota J, Nelson E, et al. Passive transfer of virus-specific antibodies confers protection against reproductive failure induced by a virulent strain of porcine reproductive and respiratory syndrome virus and establishes sterilizing immunity[J]. Virology, 2002, 302(1): 9～20.
36 Ostrowski M, Galeota J, Jar A, et al. Identification of neutralizing and nonneutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP5 ectodomain[J]. Journal of Virology, 2002, 76(9): 4241～4250.
37 Puranaveja S, Poolperm P, Lertwatcharasarakul P, et al. Chinese-like strain of porcine epidemic diarrhea virus, Thailand[J]. Emerging Infectious Diseases, 2009, 15(7): 1112.
38 Suradhat S, Kesdangsakonwut S, Sada W, et al. Negative impact of porcine reproductive and respiratory syndrome virus infection on the efficacy of classical swine fever vaccine[J]. Vaccine, 2006, 24(14): 2634～2642.
39 Thanawongnuwech R, Brown G, Halbur P, et al. Pathogenesis of porcine reproductive and respiratory syndrome virus-induced increase in susceptibility to Streptococcus suis infection[J]. Veterinary Pathology Online, 2000, 37(2): 143.
40 Thanawongnuwech R, Thacker B, Halbur P, et al. Increased production of proinflammatory cytokines following infection with porcine reproductive and respiratory syndrome virus and Mycoplasma hyopneumoniae[J]. Clinical and Diagnostic Laboratory Immunology, 2004, 11(5): 901～908.
41 Thanawongnuwech R, Suradhat S. Taming PRRSV: Revisiting the control strategies and vaccine design[J]. Virus Research, 2010, 154(1): 133～140.
42 Vanhee M, Delputte P L, Delrue I, et al. Development of an experimental inactivated PRRSV vaccine that induces virus-neutralizing antibodies[J]. Veterinary Research, 2009, 40(6): 63.
43 Wu J, Li J, Tian F, et al. Genetic variation and pathogenicity of highly virulent porcine reproductive and respiratory syndrome virus emerging in China[J]. Archives of Virology, 2009, 154(10): 1589～1597.
44 Xiao Z, Batista L, Dee S, et al. The level of virus-specific T-cell and macrophage recruitment in porcine reproductive and respiratory syndrome virus infection in pigs is independent of virus load[J]. Journal of Virology, 2004, 78(11): 5923～5933.
45 Zhou L, Zhang J, Zeng J, et al. The 30-amino-acid deletion in the Nsp2 of highly pathogenic porcine reproductive and respiratory syndrome virus emerging in China is not related to its virulence[J]. Journal of Virology, 2009, 83(10): 5156～5167.
46 Zuckermann F A, Garcia E A, Luque I D, et al. Assessment of the efficacy of commercial porcine reproductive and respiratory syndrome virus (PRRSV) vaccines based on measurement of serologic response, frequency of gamma-IFN-producing cells and virological parameters of protection upon challenge[J]. Veterinary Microbiology, 2007, 123(1): 69～85.

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