›› 2013, Vol. 40 ›› Issue (S1): 74-77.

• 安全检测 • 上一篇    下一篇

PCR技术在乳及乳制品致病菌检测中的应用

柳洪芳, 贺显书, 段余君, 杨宇, 韩国   

  1. 黑龙江省农垦科学院测试化验中心, 黑龙江佳木斯 154007
  • 收稿日期:2013-08-23 出版日期:2013-09-30 发布日期:2013-11-29
  • 作者简介:柳洪芳(1981—),女,吉林人,硕士生,工程师,研究方向:转基因和食品检测。

Application of PCR Technique for Detection of Pathogenic Bacteria in Milk and Dairy Products

LIU Hong-fang, HE Xian-shu, DUAN Yu-jun, YANG Yu, HAN Guo   

  1. Center of Test and Chemical Examination, Heilongjiang Land Reclamation Academy of Science, Jiamusi 154007
  • Received:2013-08-23 Online:2013-09-30 Published:2013-11-29

摘要: PCR技术是1985年诞生的一项DNA体外扩增技术,具有特异性强、敏感性高、操作简便、快速高效等特点,被广泛地应用于生物科学的各个领域。作者介绍了PCR技术的基本原理及其在几种致病菌如金黄色葡萄球菌、沙门氏菌、志贺氏菌、单核细胞增生李斯特氏杆菌及其他一些有害微生物在乳及乳制品检测中的应用。

关键词: PCR; 致病菌; 乳及乳制品; 检测

Abstract: PCR technology is an in vitro DNA amplification technique born in 1985,with specificity,high sensitivity,simple, and rapid characteristics and has been widely used in various fields of biological sciences.The author describes the basic principles of PCR technology,its application in several pathogenic bacteria such as Staphylococcus aureus,Salmonellla spp,Shigella,Listeria monocytogenes and other harmful microorganisms detection in milk and dairy products.

Key words: PCR; pathogenic bacteria; milk and dairy products; detection

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