中国畜牧兽医 ›› 2013, Vol. 40 ›› Issue (8): 112-115.

• 生理生化 • 上一篇    下一篇

笼养成年蛋鸡盲肠中乳酸菌的分离与鉴定

王秋菊, 崔一喆, 刘胜军, 周瑞进   

  1. 黑龙江八一农垦大学动物科技学院, 黑龙江大庆 163319
  • 收稿日期:2013-01-23 出版日期:2013-08-20 发布日期:2013-08-16
  • 通讯作者: 崔一喆
  • 作者简介:王秋菊(1979- ),女,山东人,博士,讲师,研究方向:动物肠道营养调控。
  • 基金资助:

    黑龙江省教育厅面上项目;黑龙江八一农垦大学校科研启动金(2012B-05)。

Isolation and Identification of Lactic Acid Bacteria in Cecum of Laying Hens Fed in Cage

WANG Qiu-ju, CUI Yi-zhe, LIU Sheng-jun, ZHOU Rui-jin   

  1. College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China
  • Received:2013-01-23 Online:2013-08-20 Published:2013-08-16

摘要: 试验从笼养蛋鸡盲肠内容物中分离乳酸菌,采用MRS平板分离乳酸菌,经过革兰氏染色、菌落形态观察及糖发酵生化管鉴定为乳酸菌种属。结果显示,分离出15株革兰氏阳性菌,经抑菌试验选出7株乳酸菌,发酵生化鉴定为绿色乳杆菌、瑞士乳杆菌、果糖乳杆菌、德氏乳杆菌、肠膜明串珠菌葡聚糖亚种、假肠膜明串球菌和肠膜明串珠菌乳脂亚种。

关键词: 鸡; 乳酸菌; 盲肠; 分离鉴定

Abstract: Lactobaccillus were isolated from cecal contents of laying hens fed in cage and identified by Gram staining, strain morphous observing and biochemistry fermentation. The results showed that 15 strains were G+. Selected by antibacterial activity and identified by biochemistry fermentation with different sugar, 7 lactic acid bacteria were identified, and they were Lactobacillus viridescens, Lactobacillus helveticus, Lactobacillus plantarum, Deshi Lactobacillus, Leuconostoc mesenteroides dextran, Leuconostoc mesenteroides and Leuconostoc mesenteroides subsp cremoris, respectively.

Key words: laying hens; lactic acid bacteria; cecum; isolation and identification

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