荷斯坦牛和西门塔尔牛冻精品质及其体外受精后早期胚胎发育的比较研究

›› 2013, Vol. 40 ›› Issue (6): 161-164.

荷斯坦牛和西门塔尔牛冻精品质及其体外受精后早期胚胎发育的比较研究

李晓霞¹, 汪浩², 曹平华¹, 禹学礼¹, 栗颖华¹

1. 河南科技大学动物科技学院, 河南洛阳 471003;
2. 河南农业经济学校, 河南洛阳 471002

收稿日期:2013-01-18 出版日期:2013-06-20 发布日期:2013-06-20
作者简介:李晓霞(1979-),女,河南人,博士,讲师,研究方向:哺乳动物的胚胎工程与性别控制。
基金资助:
河南科技大学博士科研启动基金(09001551);国家自然科学基金(31201801;31172207)。

Comparison on Sperm Quality and Embryos Development after in vitro Fertilization of Bovine Oocytes with Frozen-thawed Sperm from Holstein and Simmental Bulls

LI Xiao-xia¹, WANG Hao², CAO Ping-hua¹, YU Xue-li¹, LI Ying-hua¹

1. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China;
2. Henan Agroeconomical School, Luoyang 471002, China

Received:2013-01-18 Online:2013-06-20 Published:2013-06-20

摘要/Abstract

摘要： 为探讨荷斯坦牛和西门塔尔牛冻精的精液品质及体外受精后胚胎发育能力的差异,利用目测法、低渗膨胀法和考马斯亮蓝染色法评估了荷斯坦牛和西门塔尔牛冻精的活力、质膜完整率和顶体完整率,并比较了二者冻精体外受精后胚胎的卵裂率和囊胚率。结果表明,荷斯坦牛和西门塔尔牛冻精的活力(30.4%和27.2%)、质膜完整率(41.96%和36.22%)和顶体完整率(77.02%和73.02%)均无显著差异(P>0.05),但荷斯坦牛冻精体外受精后的卵裂率(57.5%和48.6%)和囊胚率(30.3%和23.2%)显著高于西门塔尔牛冻精(P<0.05)。提示,不同品种公牛精液体外受精后的发育能力有显著差异(P>0.05)。

关键词: 荷斯坦牛; 西门塔尔牛; 精液品质; 体外受精

Abstract: The objectives of this study were to investigate differences in semen quality and development of embryos after in vitro fertilization with frozen-thawed sperm from either Holstein or Simmental bulls. Progressive motility, plasma membrane integrity and acrosome integrity rates of of frozen-thawed sperm from Holstein and Simmental bulls were assessed using visual observation, the hypoosmotic swelling test and Coomassie Blue staining method, and compared the cleavage and blastocyst rates after IVM/IVF with sperm of Holstein and Simmental bulls. The results showed that progressive motility (30.4% and 27.2%),plasma membrane integrity (41.96% and 36.22%) and acrosome integrity rates (77.02% and 73.02%) of frozen-thawed sperm from Holstein and Simmental bulls were all no significant differences (P>0.05), the cleavage (57.5% and 48.6%) and blastocyst rates (30.3% and 23.2%) after IVM/IVF with sperm of Holstein bulls resulted in significant improvement over sperm of Simmental bulls (P<0.05). It was concluded that there were significant differences on embryo developmental competence of in vitro fertilization using different bull semen.

Key words: Holstein bull; Simmental bull; semen quality; in vitro fertilization

中图分类号:

S823

李晓霞, 汪浩, 曹平华, 禹学礼, 栗颖华. 荷斯坦牛和西门塔尔牛冻精品质及其体外受精后早期胚胎发育的比较研究[J]. , 2013, 40(6): 161-164.

LI Xiao-xia, WANG Hao, CAO Ping-hua, YU Xue-li, LI Ying-hua. Comparison on Sperm Quality and Embryos Development after in vitro Fertilization of Bovine Oocytes with Frozen-thawed Sperm from Holstein and Simmental Bulls[J]. , 2013, 40(6): 161-164.

参考文献

1 刘东军,廛洪武,Mal B,等.不同种公牛精液对牛卵母细胞体外受精效果影响的研究[J].内蒙古大学学报(自然科学版),2000,31(3):307~310.
2 赵永聚.舍饲条件下小尾寒羊与无角陶赛特及萨福克种公羊精液品质的比较[J].甘肃畜牧兽医,2000,30(3):6~7.
3 郝爱玲,阿淑艳,王昆,等.不同品种种公牛精液品质的差异分析[J].黑龙江畜牧科技,1997,4:20~21.
4 Panayiotis M Z,郭年藩,张才乔.牛精液保存和质量评定[J].中国畜牧兽医,1990,1:13~16.
5 Hoflack G,Opsomer G,Rijsselaere T,et al.Comparison of computer-assisted sperm motility analysis parameters in semen from Belgian Blue and Holstein-Friesian bulls[J].Reprod Domest Anim,2007,42(2):153~161.
6 Hoflack G,Van Soom A,Maes D,et al.Breeding soundness and libido examination of Belgian Blue and Holstein Friesian artificial insemination bulls in Belgium and the Netherlands[J].Theriogenology,2006,66(2):207~216.
7 Januskauskas A,Gil J,Söderquist L,et al.Effect of cooling rates on post-thaw sperm motility, membrane integrity, capacitation status and fertility of dairy bull semen used for artificial insemination in sweden[J].Theriogenology,1999,52(4):641~658.
8 Kathiravan P,Kalatharan J,John E M,et al.Computer automatedmotion analysis of crossbred bull spermatozoa and its relationship with in vitro fertility in zona-free hamster oocytes[J].Anim Reprod Sci,2008,104:9~17.
9 Kasimanickam R,Kasimanickam V,Pelzer K D,et al.Effect of breed and sperm concentration on the changes in structural, functional and motility parameters of ram-lamb spermatozoa during storage at 4℃[J].Animal Reproduction Science,2007,101(1~2):60~73.
10 Milko S,Ivan N,Maria I K,et al.Cryopreservation of ram sperm from autochthonous breeds during a non-mating season[J].Journal of Central European Agriculture,2007,7(4):677~682.
11 Rijsselaere T,Van Soom A,Maes D,et al.Use of the sperm quality analyzer (SQA Ⅱ-C) for the assessment of dog sperm quality[J].Reprod Dom Anim,2002,37:158~163.
12 Saacke R G,White J M.Semen quality tests and their relationship to fertility[M]. In: Proceedings of the 14th Technical Conference on AI Reproduction,1972.
13 Saeki K,Nagao Y,Hoshi M,et al.Effects of heparin, sperm concentration and bull variation on in vitro fertilization of bovine oocytes in a protein-free medium[J].Theriogenology,1995,43:751~759.
14 Setchell B P,Maddocks S S,Anatomy D E B.Vasculature, innervation and fluids of the male reproductive tract[M].Second Edition. In: The Physiology of Reproduction, 1993.
15 Zi X D,Lu H,Yin R H,et al.Development of embryos after in vitro fertilization of bovine oocytes with sperm from either yaks(Bos grunniens) or cattle (Bos taurus)[J].Animal Reproduction Science,2008,108(1~2):208~215.

[1]	张笑梦, 焦安惠, 王玉琪, 方南洙, 金庆国. 添加罗格列酮对小鼠卵母细胞氧化还原稳态的影响[J]. 中国畜牧兽医, 2023, 50(5): 1818-1827.
[2]	宋承磊, 张哲, 韦怡林, 杨松柏, 豆雅晴, 李晨雷, 刘蓥珂, 李新建, 杨峰, 李秀领, 王克君, 乔瑞敏, 韩雪蕾. 不同品种、季节和月龄对公猪精液品质的影响[J]. 中国畜牧兽医, 2023, 50(5): 1938-1946.
[3]	杜朝晖, 葛梓豪, 游君怡, 刘晓玮, 梁国栋, 孙世铎, 胡建宏, 李晓. 丁酸梭菌和丁酸钠对高脂饮食诱导雄性小鼠生殖损伤的影响[J]. 中国畜牧兽医, 2023, 50(4): 1603-1616.
[4]	曾治钦, 娄文琦, 许静漪, 马烨桦, 张永福, 任小丽, 陈少侃, 闫磊, 郭刚, 张震, 王雅春. 泌乳期中国荷斯坦牛转化孕酮浓度的影响因素分析及其表型预测[J]. 中国畜牧兽医, 2023, 50(1): 186-197.
[5]	张雨阳, 曲星霖, 陈璇, 吕艳秋, 曹立朋, 张君正, 金一. 泛素激活酶抑制剂对猪精子获能状态、膜重构及体外受精的影响[J]. 中国畜牧兽医, 2023, 50(1): 210-219.
[6]	张笑科, 廖伟莉, 李婷婷, 李瑶, 李加琪, 黄翔, 张豪. 采精员和猪舍环境参数对杜洛克公猪精液品质的影响[J]. 中国畜牧兽医, 2023, 50(1): 220-229.
[7]	徐昊祺, 许静漪, 胡丽蓉, 张帆, 罗汉鹏, 张海亮, 师睿, 李想, 刘林, 刘巧香, 郭刚, 王雅春. 中国荷斯坦牛PIK3CB基因多态性及其与繁殖和产奶性状的关联分析[J]. 中国畜牧兽医, 2022, 49(9): 3438-3452.
[8]	袁崇善, 王军, 吕文发. 多不饱和脂肪酸对动物精液品质影响的研究进展[J]. 中国畜牧兽医, 2022, 49(4): 1422-1429.
[9]	罗兰, 崔超, 董瑞兰, 刘浩域, 柳小斌, 于光辉. 不同浓度芦丁和冷冻速率对猪精子冷冻保存效果的研究[J]. 中国畜牧兽医, 2022, 49(3): 982-991.
[10]	王宇, 赵予晗, 焦安惠, 王玉琪, 高青山. 乙草胺对小鼠卵母细胞体外成熟的影响[J]. 中国畜牧兽医, 2022, 49(10): 3909-3917.
[11]	罗汉鹏, 窦金焕, 安涛, 陈少侃, 王雅春. 基于荷斯坦牛群体基因组数据填充软件的准确性比较(Minimac 3与Beagle 5.1)[J]. 中国畜牧兽医, 2021, 48(5): 1664-1671.
[12]	孙宇辰, 许龙, 殷佳辉. 西门塔尔牛牙龈间充质干细胞的分离鉴定及诱导分化[J]. 中国畜牧兽医, 2021, 48(3): 855-864.
[13]	王腾飞, 张燕, 王彦平, 赵善江, 朱化彬, 王玲玲, 麻柱, 戴蕴平, 庞云渭. 奶牛活体采卵-体外受精效率的影响因素研究[J]. 中国畜牧兽医, 2021, 48(2): 574-580.
[14]	张雨阳, 汪秋月, 吕艳秋, 陈璇, 徐海峰, 张正文, 金一. 谷氨酰胺替代部分甘油对冻融猪精子获能和体外受精能力的影响[J]. 中国畜牧兽医, 2021, 48(11): 4125-4132.
[15]	艾日夏提·地里夏提, 周正娜, 张也, 刘志强, 章亚峰, 赵茜, 阿布力孜·吾斯曼. 藏红花素对小鼠卵母细胞体外成熟能力的影响[J]. 中国畜牧兽医, 2021, 48(10): 3734-3740.