北方地区荷斯坦牛体内超数排卵反应性状群体特征及影响因素分析

doi:10.16431/j.cnki.1671-7236.2024.11.020

中国畜牧兽医 ›› 2024, Vol. 51 ›› Issue (11): 4851-4859.doi: 10.16431/j.cnki.1671-7236.2024.11.020

• 遗传繁育 • 上一篇

北方地区荷斯坦牛体内超数排卵反应性状群体特征及影响因素分析

黄越川^1,2, 张海亮², 刘展鹏³, 赵善江⁴, 孙伟⁵, 李喜和⁵, 王林⁶, 朱化彬⁴, 黄锡霞¹, 王雅春²

1. 新疆农业大学动物科学学院, 乌鲁木齐 830052;
2. 中国农业大学动物科学技术学院, 农业农村部动物遗传育种与繁殖(家畜)重点实验室, 畜禽育种国家工程实验室, 畜禽生物育种全国重点实验室, 北京 100193;
3. 内蒙古国家乳业技术创新中心, 呼和浩特 010020;
4. 中国农业科学院北京畜牧兽医研究所, 畜禽生物育种全国重点实验室, 北京 100193;
5. 内蒙古赛科星家畜种业与繁育生物技术研究院有限公司, 呼和浩特 011517;
6. 北京优源牧业有限公司, 北京 101308

收稿日期:2024-02-05 发布日期:2024-10-31
通讯作者: 黄锡霞 E-mail:au-huangxixia@163.com
作者简介:黄越川,E-mail:hyccau@cau.edu.cn;王雅春,E-mail:wangyachun@cau.edu.cn。
基金资助:
低碳排放与抗热应激奶牛培育关键技术研究及奶羊引种扩繁技术创新(2022-科研攻关-3);国家现代农业产业技术体系(CARS-36);长江学者和创新团队发展计划(IRT_15R62)

Analysis of the Population Characteristics and Influencing Factors of Superovulation Response Traits in Northern Chinese Holstein Cattle

HUANG Yuechuan^1,2, ZHANG Hailiang², LIU Zhanpeng³, ZHAO Shanjiang⁴, SUN Wei⁵, LI Xihe⁵, WANG Lin⁶, ZHU Huabin⁴, HUANG Xixia¹, WANG Yachun²

1. College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China;
2. State Key Laboratory of Farm Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproductive of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;
3. National Center of Technology Innovation for Dairy, Hohhot 010020, China;
4. Key Laboratory of Animal(Poultry) Genetics Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, Institute of Animal Science of CAAS, Beijing 100193, China;
5. Inner Mongolia Saikexing Institute of Breeding and Reproductive Biotechnology in Domestic Animal, Hohhot 011517, China;
6. Beijing Youyuan Animal Husbandry Co., Ltd., Beijing 101308, China

Received:2024-02-05 Published:2024-10-31

摘要/Abstract

摘要： 【目的】体内超数排卵(以下简称超排)是现代奶牛繁育关键技术之一，对于奶牛群体遗传改良具有重要意义。试验旨在揭示超排反应性状在荷斯坦牛群体中的表型规律及主要影响因素，以期为奶牛超排反应性状的遗传规律解析提供表型研究基础。【方法】本研究收集了宁夏和内蒙古地区共9个牧场2021年3月至2023年8月间1 842头荷斯坦牛的1 855条体内超排记录，对总胚胎数、可移植胚胎数、可移植胚胎率、未受精卵数、未受精卵率、退化胚胎数和退化胚胎率共7个性状进行了统计分析，使用SAS 9.2软件中Mixed程序分析了非遗传因素对7个性状的影响，并使用SPSS 21.0软件的双变量相关程序计算了7个超排反应性状间的Pearson相关系数。【结果】本研究群体中，荷斯坦牛体内超排生产的总胚胎数、可移植胚胎数和可移植胚胎率的平均值分别为9.49枚、6.01枚和61.95%，未受精卵数和未受精卵率的平均值分别为1.50枚和17.16%，退化胚胎数和退化胚胎率的平均值分别为2.29枚和23.34%。各超排反应性状间存在不同程度的表型相关，相关系数介于－0.629～0.882之间。除可移植胚胎率外其余性状均受季节因素的显著影响(P＜0.05)；激素类型及剂量对7个超排反应性状均有显著影响(P＜0.05)；冻精使用剂量显著影响了可移植胚胎数、退化胚胎数、未受精卵数和未受精卵率(P＜0.05)；供体月龄仅对可移植胚胎率和退化胚胎率有显著影响(P＜0.05)。【结论】中国荷斯坦牛的超排反应性状表型与世界平均水平相近，并受到多种非遗传因素的影响。研究结果为进一步对奶牛超排反应性状进行遗传分析奠定了模型基础。

关键词: 荷斯坦牛; 超数排卵; 群体特征; Pearson相关性

Abstract: 【Objective】 In vivo superovulation is one of the key technologies in modern dairy cattle breeding,which is of great significance for genetic improvement of dairy cattle populations.This study was conducted to reveal the phenotypic characteristics and main influencing factors in Chinese Holstein cattle,so as to provide foundation for the genetic analysis of superovulation response traits in dairy cattle. 【Method】 In this study,1 855 in vivo superovulation records of 1 842 Chinese Holstein cattle from 9 pastures in Ningxia and Inner Mongolia from March 2021 to August 2023 were collected,7 superovulation response traits were statistically analyzed,including total number of oocytes,number and percentage of transferable embryos,number and percentage of unfertilized oocytes,and number and percentage of degenerated embryos.The Mixed model of SAS 9.2 software was used to analyze the influencing factors on each trait,and the Pearson correlation coefficient among 7 superovulation response traits were calculated using a bivariate correlation program of SPSS 21.0 software. 【Result】 The average number of total oocytes and transferable embryos in Chinese Holstein cattle were 9.49 and 6.01,while the average percentage of transferable embryos was 61.95%.The average number and percentage of unfertilized oocytes were 1.50 and 17.16%,while the average number and percentage of degenerated embryos were 2.29 and 23.34%,respectively.There was varying degrees of phenotypic correlation among 7 superovulation response traits,with correlation coefficients ranging from ―0.629 to 0.882.Seasons had significant influence on all traits except for the percentage of transferable embryos (P<0.05). Hormone types and doses had significant impact on 7 superovulation response traits (P＜0.05).Semen dosage significantly affected the number of transferable embryos,degenerated embryos,and the number and percentage of unfertilized oocytes (P＜0.05).Donor age had a significant impact only on the percentage of transferable embryos and degenerated embryos (P＜0.05). 【Conclusion】 The phenotype of superovulation response traits in Chinese Holstein cattle was similar to the average level in global cattle.The traits were influenced by various non-genetic factors.The results laid a model foundation for further genetic analysis of superovulation response traits in dairy cattle.

Key words: Chinese Holstein cattle; superovulation; population characteristics; Pearson correlation

中图分类号:

S814.4

黄越川, 张海亮, 刘展鹏, 赵善江, 孙伟, 李喜和, 王林, 朱化彬, 黄锡霞, 王雅春. 北方地区荷斯坦牛体内超数排卵反应性状群体特征及影响因素分析[J]. 中国畜牧兽医, 2024, 51(11): 4851-4859.

HUANG Yuechuan, ZHANG Hailiang, LIU Zhanpeng, ZHAO Shanjiang, SUN Wei, LI Xihe, WANG Lin, ZHU Huabin, HUANG Xixia, WANG Yachun. Analysis of the Population Characteristics and Influencing Factors of Superovulation Response Traits in Northern Chinese Holstein Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(11): 4851-4859.

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北方地区荷斯坦牛体内超数排卵反应性状群体特征及影响因素分析

Analysis of the Population Characteristics and Influencing Factors of Superovulation Response Traits in Northern Chinese Holstein Cattle

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