催乳复合物、胰岛素样生长因子-Ⅰ对奶牛乳腺上皮细胞中AKT-1和mTOR表达的作用

›› 2013, Vol. 40 ›› Issue (3): 23-27.

催乳复合物、胰岛素样生长因子-Ⅰ对奶牛乳腺上皮细胞中AKT-1和mTOR表达的作用

史琳琳, 赵锋, 高学军, 毕微微, 武彩霞, 李庆章

东北农业大学乳品科学教育部重点实验室,黑龙江哈尔滨 150030

收稿日期:2012-08-10 出版日期:2013-03-20 发布日期:2013-03-19
通讯作者: 李庆章(1953-),男,河北人,教授,博士生导师,研究方向:泌乳生物学与乳腺功能调控。E-mail:qzli@neau.edu.cn;Tel:0451-55191666 E-mail:qzli@neau.edu.cn
作者简介:史琳琳(1987-),女,内蒙古人,硕士,研究方向:泌乳生物学与乳腺功能调控。
基金资助:
国家重点基础研究发展(973)计划课题(2011CB100804)。

Effection of DIP and IGF-Ⅰ on the Expression of AKT-1 and mTOR Genes in Bovine Mammary Epithelial Cells

SHI Lin-lin, ZHAO Feng, GAO Xue-jun, BI Wei-wei, WU Cai-xia, LI Qing-zhang

Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China

Received:2012-08-10 Online:2013-03-20 Published:2013-03-19

摘要/Abstract

摘要： 信号转导分子AKT-1、mTOR对于乳腺上皮细胞中乳蛋白的转录和翻译至关重要,胰岛素、地塞米松和催乳素组成的催乳复合物(DIP)是包括AKT-1/mTOR在内的多种泌乳信号途径激活的必要条件。采用qRT-RCR和Western blotting分别检测不同培养时间点奶牛乳腺上皮细胞AKT-1、mTOR的mRNA和蛋白质水平表达情况,结果显示,在DIP培养条件下,AKT-1和mTOR表达量均上升。添加胰岛素样生长因子-Ⅰ(IGF-Ⅰ)后进一步促进了AKT-1 mRNA和蛋白质的表达;同时,mTOR mRNA和蛋白质水平的表达也增加,且均有显著差异(P<0.05),结果表明DIP存在时,IGF-Ⅰ上调AKT-1和mTOR的表达,增强了AKT/mTOR途径。

关键词: 乳腺上皮细胞; IGF-Ⅰ; DIP; mRNA; 蛋白质

Abstract: Signal transduction molecules AKT-1 and mTOR were essential for milk protein gene transcription and translation in mammary epithelial cells.Insulin,dexamethasone and prolactin composed of prolactin complex (DIP), the variety of lactation signal pathway activation was a necessary condition,including AKT-1/mTOR. In this study,the qRT-RCR and Western blotting were used to test above-mentioned two genes's expression level of mRNA and protein in different time points.The results showed that IGF-Ⅰ promoted significantly the expression levels of mRNA and protein for AKT-1 gene in the basis of DIP compared to the absence of IGF-Ⅰ(P<0.05), and it also promoted significantly the expression of mTOR gene in mRNA and protein levels(P<0.05).

Key words: mammary epithelial cells; IGF-Ⅰ; DIP; mRNA; protein

中图分类号:

S816.79

史琳琳, 赵锋, 高学军, 毕微微, 武彩霞, 李庆章. 催乳复合物、胰岛素样生长因子-Ⅰ对奶牛乳腺上皮细胞中AKT-1和mTOR表达的作用[J]. , 2013, 40(3): 23-27.

SHI Lin-lin, ZHAO Feng, GAO Xue-jun, BI Wei-wei, WU Cai-xia, LI Qing-zhang. Effection of DIP and IGF-Ⅰ on the Expression of AKT-1 and mTOR Genes in Bovine Mammary Epithelial Cells[J]. , 2013, 40(3): 23-27.

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