›› 2013, Vol. 40 ›› Issue (2): 130-132.

• 遗传繁育 • 上一篇    下一篇

边鸡胰岛素生长因子结合蛋白-3基因外显子2MspⅠ位点对繁殖性状的遗传效应分析

李青1, 刘学1, 刘敏1, 张跟喜1, 丁馥香2, 张丽2, 王金玉1   

  1. 1. 扬州大学动物科学与技术学院,江苏扬州 225009;
    2. 山西省农业科学院畜牧兽医研究所,山西太原 030032
  • 收稿日期:2012-08-01 出版日期:2013-02-20 发布日期:2013-02-06
  • 通讯作者: 张跟喜,讲师。Tel:0514-87997204
  • 作者简介:李青(1991-),女,江苏人,学士,研究方向:动物科学。
  • 基金资助:
    扬州大学大学生科技创新基金;国家自然科学基金(31201793);江苏高校优势学科建设工程项目;山西省科技攻关(20100311041);国家肉鸡产业技术体系(nycytx-42-G1-05)。

Genetic Effect Analysis of the MspⅠ Locus in Exon 2 of the IGFBP-3 Gene on the Reproduction Traits in the Bian Chicken

LI Qing1, LIU Xue1, LIU Min1, ZHANG Gen-xi1, DING Fu-xiang2, ZHANG Li2, WANG Jin-yu1   

  1. 1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
    2. Institute of Animal Husbandry and Veterinary, Shanxi Academy of Agricultural Sciences, Taiyuan 030032, China
  • Received:2012-08-01 Online:2013-02-20 Published:2013-02-06

摘要: 本研究采用PCR-RFLP技术检测边鸡类胰岛素生长因子结合蛋白-3(insulin-like growth factor binding proteins-3,IGFBP-3)基因外显子2 MspⅠ位点的单核苷酸多态性,并与边鸡的繁殖性状进行相关分析。结果表明,在边鸡IGFBP-3基因的1087 bp处检测到1个C→T的突变,显示3种基因型(CC、CT和TT),C等位基因为优势等位基因。最小二乘分析结果表明,CT基因型的开产体重显著高于CC基因型(P<0.05)。不同基因型对其余4个繁殖性状(开产日龄、开产蛋重、300日龄产蛋数及300日龄蛋重)均无显著影响(P>0.05)。

关键词: 边鸡; IGFBP-3基因; 繁殖性状

Abstract: Single nucleotide polymorphisms (SNPs) of the MspⅠ locus in exon 2 of the IGFBP-3 gene was detected in the Bian chicken by PCR-RFLP. The relationship with reproduction traits in the Bian chicken was also analysed. The results showed that a C→T mutation in the 1087 bp of the IGFBP-3 gene was detected. Three genotypes (CC, CT and TT) were displayed, and the allele C was the predominant allele. The least square means analysis showed Bian chickens of the CT genotype had significantly higher body weight at first egg than those of CC genotytpe (P<0.05). The differences for the other four reproduction traits (age at first egg, weight of first egg, egg number at 300 days and egg weight at 300 days) among the 3 genotypes in Bian chicken were not significant (P>0.05).

Key words: Bian chicken; IGFBP-3 gene; reproduction traits

中图分类号: