关键词: 蝇蛆; 抗菌肽; 诱导; 灭活; 抗菌性能

Abstract: In order to explore the effect of inactivated Salmonella induced maggot antimicrobial peptides, maggot were cultivated with inactivated of Salmonella and Salmonella, extracting antimicrobial peptides from maggot. The concentration of maggot antimicrobial peptides and antibacterial property were determined at different time. Meanwhile bacterial content of maggot in vivo was counted by colony forming units (CFU). The results showed that the concentration of induced antimicrobial peptides was significant higher than the non-induced group (P<0.05), and the biggest concentration of antimicrobial peptides was showed at 24 h;inhibition zone diameter of induction group was significant bigger than non-induced group for Salmonella (P<0.05), but less than antibiotic group (P<0.05), and the value of MIC was 0.5 mg/mL;the bacterial content of maggot in vivo was 1.6×10⁵ CFU/g, was lower than the national standard of the number of bacteria in the feed 2×10⁶ CFU/g. The experiments indicated that the expression of maggot antimicrobial peptides could be enhanced by induction of inactivated Salmonella, and the antimicrobial peptides showed a strong anti-bacterial property and security.

Key words: maggots; antimicrobial peptides; induction; inactivation; antibacterial property