H9亚型禽流感病毒分离鉴定及鸡新城疫、禽流感(H9亚型)、禽腺病毒病三联灭活疫苗对其免疫保护效果研究

doi:10.16431/j.cnki.1671-7236.2023.12.023

摘要/Abstract

摘要： 【目的】研究鸡新城疫、禽流感(H9亚型)、禽腺病毒病(Ⅰ群,4型)(简称:新流腺)三联灭活疫苗(La Sota株+BX13株+DC株)对禽流感病毒(AIV)流行毒株的免疫保护效果,为新流腺三联灭活疫苗的研发提供数据支撑。【方法】本研究对2019－2022年分别对从河北石家庄、北京密云、河北衡水、广西南宁发病鸡群中采集的病料进行AIV分离鉴定;利用MegAlign软件分析BX13株和2019－2022年分离株之间的相似性及亲缘关系;采用新流腺三联灭活疫苗免疫SPF鸡,免疫后21 d测定H9亚型 AIV HI抗体,同时用同源BX13株和本试验中AIV分离株进行攻毒试验,检测新流腺三联灭活疫苗对H9亚型AIV流行毒株的保护效果。【结果】2019－2022年从河北、北京和广西发病鸡群中分离获得4株AIV,均为H9亚型AIV,分别命名为SJZ19、MY20、HS21和NN22株;BX13株与2019－2022年4个分离株的相似性较高,在95.3%~96.3%之间;BX13株和4个分离株均属于DK-HK-Y280-97所在亚群的分支;新流腺三联灭活疫苗免疫后,各免疫组鸡H9亚型AIV HI抗体效价明显上升,达到9.2~9.9 log2,而对照组鸡抗体为阴性;用同源BX13株攻毒后,对照组鸡100%(5/5)阳性,免疫组鸡100%(10/10)阴性,保护率为100%;用4株H9亚型AIV分离株攻毒后,对照组鸡100%(5/5)阳性,免疫组鸡90%(9/10)~100%(10/10)阴性,保护率为90%~100%。【结论】以BX13株制备出的新流腺三联灭活疫苗对目前的流行毒株具有较好的免疫保护效果。

关键词: H9亚型禽流感病毒; 流行毒株; 三联灭活疫苗; 免疫保护

Abstract: 【Objective】 The aim of the experiment was to study the immune protection effect of the inactivated trivalent vaccine (La Sota strain＋BX13 strain＋DC strain) of Newcastle disease,avian influenza (H9 subtype),and Avian adenovirus disease (group Ⅰ,type 4) on epidemic strains of Avian influenza virus (AIV),and provide the data for supporting the research of the inactived trivalent vaccine.【Method】 In this study,AIV was isolated and identified in samples collected from infected chickens in Shijiazhuang-Hebei,Miyun-Beijing,Hengshui-Hebei and Nanning-Guangxi during 2019-2022.The MegAlign software was used to analyze the similarity and phylogenetic relationship among the BX13 and the isolated strains from 2019 to 2022.SPF chickens were immunized with the inactivated trivalent vaccine and the serum was collected to determine the HI titer 21 d after immunization.At the same time,a challenge test was conducted using the homologous BX13 strain and the AIV isolates in this experiment to test the protective effect of the inactivated trivalent vaccine on the H9 subtype AIV epidemic strains.【Result】 Four strains of AIV were isolated from Hebei,Beijing and Guangxi from 2019 to 2022,all of which were H9 subtype AIVs,named SJZ19,MY20,HS21 and NN22 strains, respectively.The similarity among the BX13 strain and the four isolated strains from 2019 to 2022 was relatively high,ranging from 95.3% to 96.3%.BX13 strain and 4 isolates all belonged to the branch of DK-HK-Y280-97.After being immunized with the inactivated trivalent vaccine,the HI antibody titers of the H9 subtype AIV in chickens of each immunization group significantly increased,reaching 9.2-9.9 log2,while the control group showed negative antibodies.After being challenged with homologous BX13 strain,100% (5/5) of chickens in control group were positive,while 100% (10/10) of chickens in immune group were negative,with a protection rate of 100%.After being challenged with four H9 subtype AIV isolates,chickens in control group were 100% (5/5) positive,while chickens in immune group were 90% (9/10) to 100% (10/10) negative,with a protection rate of 90% to 100%.【Conclusion】 The inactivated trivalent vaccine prepared by BX13 strain had good immune protection effect on the current epidemic virus strains.

Key words: H9 subtype Avian influenza virus; epidemic strains; inactivated trivalent vaccine; immune protection

中图分类号:

S852.65

靳换, 涂敏, 史爱华, 赵蕾, 沈佳, 习硕, 张建伟, 章振华. H9亚型禽流感病毒分离鉴定及鸡新城疫、禽流感(H9亚型)、禽腺病毒病三联灭活疫苗对其免疫保护效果研究[J]. 中国畜牧兽医, 2023, 50(12): 5013-5021.

JIN Huan, TU Min, SHI Aihua, ZHAO Lei, SHEN Jia, XI Shuo, ZHANG Jianwei, ZHANG Zhenhua. Isolation,Identification of H9 Subtype Avian Influenza Virus and Immune Protection Effect of Inactivated Trivalent Vaccine of Newcastle Disease,Avian Influenza (H9 Subtype),and Avian Adenovirus Disease Against the Viruses[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(12): 5013-5021.

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