“乳宁散”对小鼠急性和大鼠亚慢性毒性的试验研究

›› 2013, Vol. ›› Issue (5): 129-133.

“乳宁散”对小鼠急性和大鼠亚慢性毒性的试验研究

王国庆, 王旭荣, 张凯, 张景艳, 王学智, 孟嘉仁, 常瑞祥, 杨志强, 李建喜

中国农业科学院兰州畜牧与兽药研究所, 甘肃省中兽药工程技术研究中心, 农业部兽用药物创制重点实验室, 甘肃省新兽药工程重点实验室, 甘肃兰州 730050

收稿日期:2013-01-23 出版日期:2013-05-20 发布日期:2013-05-27
通讯作者: 李建喜(1971-),男,博士,副研究员,从事兽医药理毒理学研究。E-mail:lzjianxil@163.com;Tel:0931-2115256 E-mail:lzjianxil@163.com
作者简介:王国庆(1987-),男,河南人,硕士生,研究方向:兽医药理与毒理学。
基金资助:
国家奶牛产业技术体系科学家岗位项目(CARS37);甘肃省科技重大专项计划(1102NKDA020)。

Study on Acute Toxicity Test in Mice and Sub-acute Toxicity Test in Rats of "Runingsan"

WANG Guo-qing, WANG Xu-rong, ZHANG Kai, ZHANG Jing-yan, WANG Xue-zhi, MENG Jia-ren, CHANG Rui-xiang, YANG Zhi-qiang, LI Jian-xi

Key Laboratory of New Animal Drug Project of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Engineering and Technology Research Center of TCVM of Gansu Province, Lanzhou Institute of Husbandry and Pharmaceutical Science of CAAS, Lanzhou 730050, China

Received:2013-01-23 Online:2013-05-20 Published:2013-05-27

摘要/Abstract

摘要： 通过小鼠急性毒性试验和大鼠亚慢性毒性试验评价防制奶牛隐性乳房炎中药"乳宁散"的安全性。将"乳宁散"制备成相当于原药材1.0 g/mL的水煎提取浓缩液,选取50只小鼠,随机分为5组,以5000、7500、10000、15000 mg/kg体重的剂量1次灌胃给药,观察中毒症状,记录死亡数和计算半数致死量(LD₅₀);另取40只小鼠,随机分为2组,给药组以最大浓度(1.0 g/mL)、最大容积(0.04 mL/g)1次灌胃受试药物,对照组用等体积生理盐水,给药后连续观察7 d,测定最大给药量;再取80只大鼠,随机分成药物处理高、中、低剂量组和对照组,药物组按大鼠体重3000、1500和750 mg/kg剂量灌胃给药,连续30 d,对照组用生理盐水,检测大鼠体重、血常规指标、血液生化指标、脏器系数及组织病理变化情况。结果表明,急性毒性试验各剂量组均无小鼠死亡,无法计算LD₅₀,经口给药的最大剂量为40.0 g/kg体重,表明该产品实际无毒;亚慢性毒性试验中,药物处理组大鼠体增重、血常规、血液生化指标和脏器系数与对照组相比无显著差异(P>0.05),组织病理学观察实质器官无异常病变。提示,临床合理使用中药"乳宁散"不会对靶动物产生毒性作用。

关键词: 奶牛; 隐性乳房炎; 中药; 毒性试验

Abstract: To investigate the safety of "Runingsan" for treatment and prevention of the mastitis in dairy cow, the acute and sub-acute toxicity was tested in mice and rat. "Runingsan" was extracted with water and concentrated 1.0 g/mL solution. In acute toxicity test, 50 mice were randomly divided into 5 groups, the animals were administered drug by intragastric infusion at single dose of 5000, 7500, 10000, 15000 mg/(kg·BW) respectively. Poisoning typical symptom and death cases were observedand the median lethal dose (LD₅₀) were calculated. In order to estimate the maximum dosage, 40 mice were randomly divided into the treatment group and control group group, "Runingsan" was administered intragastrically at a maximum concentration (1.0 g/mL) and maximum capacity (0.04 mL/g) with single dose in the treatment group, 0.9% saline was used in control group. After being administered, the mice were observed for 7 days. In sub-acute toxicity test, 80 rats were randomly divided into 3 drug-treated groups (high, middle and low dose) and one control group, drug was administered orally at dose of 3000, 1500 and 750 mg/(kg·d) respectively. In control group, rats were administered 0.9% NaCl. Body weight gain, routine blood test indexes, blood biochemical parameters, organ coefficient and histopathology were detected. The results showed there was not any poisoning and death cases in each group, there was no LD₅₀ in this herbal compound. "Runingsan" maximum oral dosage was over 40.0 g/(kg·BW) in mice. These data indicated that the drug was actually non-toxin. There were not significantly different in body weight gain, routine blood test, blood biochemical indexes and organ coefficient between 3 drug-treated groups and control group. The histopathological result was not any lesion in rat real organs of every group. All of these results suggested that "Runingsan" was safety for the dairy cow, which were in mastitis under the recommended dosage and course of the treatment in clinic.

Key words: dairy cow; sub-clinical mastitis; traditional Chinese veterinary medicine; toxicity test

中图分类号:

S853.73⁺2

王国庆, 王旭荣, 张凯, 张景艳, 王学智, 孟嘉仁, 常瑞祥, 杨志强, 李建喜. “乳宁散”对小鼠急性和大鼠亚慢性毒性的试验研究[J]. , 2013, (5): 129-133.

WANG Guo-qing, WANG Xu-rong, ZHANG Kai, ZHANG Jing-yan, WANG Xue-zhi, MENG Jia-ren, CHANG Rui-xiang, YANG Zhi-qiang, LI Jian-xi. Study on Acute Toxicity Test in Mice and Sub-acute Toxicity Test in Rats of "Runingsan"[J]. , 2013, (5): 129-133.

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