中国畜牧兽医

• 疾病防治 • 上一篇    下一篇

猪大肠杆菌和葡萄球菌的分离鉴定及耐药性分析

李淑红, 王京仁, 成钢, 曾文虎   

  1. 湖南文理学院生命科学学院,湖南常德 415000
  • 收稿日期:2012-01-16 出版日期:2012-08-20 发布日期:2012-08-24
  • 作者简介:李淑红(1964-),女,山东人,教授,硕士,主要从事动物科学专业的教学与研究。
  • 基金资助:

    湖南省动物学重点实验室资助(2008);湖南省高校创新平台开放基金项目(10K043);湖南省"十二五"重点建设学科(动物学)项目(2011)。

Isolation,Identification and Analysis of Drug Resistance on E. coli and Staphylococcus in Pigs

LI Shu-hong, WANG Jing-ren, CHENG Gang, ZENG Wen-hu   

  1. Department of Life Science,Hunan University of Arts and Science,Changde 415000,China
  • Received:2012-01-16 Online:2012-08-20 Published:2012-08-24

摘要: 对湘西北地区某猪场患病的仔猪的粪便及病变的脾脏、肝脏、淋巴结、肺脏、肾水肿液进行病原菌分离鉴定,并用Kirby-Bauer法测定分离菌株对15种抗菌药的敏感性。结果表明,患病仔猪分离到大肠杆菌和猪葡萄球菌两种细菌。其中大肠杆菌对庆大霉素、头孢呋肟、丁胺卡那霉素、四环素、先锋必素敏感,对氯霉素、氧哌嗪青霉素、先锋五号、头孢曲松钠、新霉素中介,对羧苄青霉素、强力霉素、青霉素、氨苄青霉素、红霉素有较高的耐药性。葡萄球菌对先锋霉素Ⅴ、丁胺卡那霉素敏感,对头孢曲松钠中介,对其他抗生素耐药。其中大肠杆菌对药物敏感率、中介率、耐药率均为33.33%;葡萄球菌的敏感率为13.33%,中介率为6.67%,耐药率为80.00%。

关键词: 猪; 大肠杆菌; 葡萄球菌; 药敏试验

Abstract: The pathogen were isolated and identified on feces of sick piglets and disease of the spleen, liver, lymph nodes, lung, kidney edema fluidin a pig farm in Hunan northwestern region. Susceptibility of 15 kinds antimicrobial drug was determined with the Kirby-Barer for isolated strains. The results showed piglets were infected with E. coli-based and Staphylococcus. Escherichia coli was sensitive to gentamicin, cephalosporins latter skin, Amikacin, tetracycline, cefoperazone sodium, to chloramphenicol, piperacillin, pioneer V, ceftriaxone, neomycin was intermediate, to carbenicillin, doxycycline, penicillin, ampicillin, erythromycin had a higher resistance. Staphylococcus was sensitive to pioneer V, Amikacin, to ceftriaxone was intermediate, on the other antibiotic was resistance. Which was 33.33% the rate of drug sensitivity, intermediate and resistance to E.coli. Which was 13.33% sensitive rate, intermediate rate was 6.67%, resistance rate was 80.00% to Staphylococcus.

Key words: pig; Escherichia coli; Staphylococcus; drug susceptibility testing

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