›› 2012, Vol. 39 ›› Issue (3): 81-84.

• 生物技术 • 上一篇    下一篇

免疫荧光检测技术及其在寄生虫检测中的应用进展

王小环1,2, 杨莲如1, 赵林立3, 吴绍强2   

  1. 1. 内蒙古农业大学兽医学院,内蒙古呼和浩特 010018;2. 中国检验检疫科学研究院,北京 100029;3. 内蒙古出入境检验检疫局,内蒙古呼和浩特 010020
  • 收稿日期:1900-01-01 修回日期:2011-12-30 出版日期:2012-03-20 发布日期:2012-03-20
  • 通讯作者: 吴绍强

Brief Introduction of Immunefluorescent Test Technology and Application Progress on Parasites Detection

WANG Xiao-huan1,2, YANG Lian-ru1, ZHAO Lin-li3, WU Shao-qiang2   

  1. 1. College of Veterinary Science,Inner Mongolia Agriculture University,Huhhot 010018,China;2. Chinese Academy of Inspection & Quarantine,Beijing 100029,China;3. Inner Mongolia Entry and Exit Inspection and Quarantine,Huhhot 010020,China
  • Received:1900-01-01 Revised:2011-12-30 Online:2012-03-20 Published:2012-03-20

摘要: 免疫荧光技术(immunofluorescence technique)是在生物化学、显微镜技术和免疫学基础上发展起来的一项检测技术,是用荧光标记的抗体或抗原与被检样品中相应的抗原或抗体结合,在显微镜下检测荧光,并对样品进行分析的方法。它把显微镜技术的精确性和免疫学检测的特异性、敏感性有机地结合在一起。这一方法的特点是特异性强、灵敏度高。作为一种快速诊断方法,目前广泛应用于病毒、细菌病的诊断,也是许多动物寄生虫病(如弓形虫病)的常规诊断方法。随着标记抗体、抗原的普及,免疫荧光技术在寄生虫病诊断方面的应用将更加广泛。

关键词: 免疫荧光技术; 寄生虫; 检测

Abstract: Immunofluorescence technique is a new detection technology which is based on biochemistry,microscopy and immunology. Its basic principle is using fluorescence-labeled antibody or antigen to combine with the antigen or antibody in the samples,and the fluorescence can be recognized under a microscope. The microscopic accuracy and the immunological specificity and sensitivity can be organically combined in this method. As a rapid diagnostic method,immunofluorescence technique is widely used in medical and biological research. It is a general method in the diagnosis of virus,bacterium or parasite caused diseases,such as Toxoplasma gondii infection. With the development of labeled antigen and antibody,immunofluorescence technique will be widely used in parasitosis diagnosis.

Key words: immunofluorescence technique; parasite; detection