›› 2012, Vol. 39 ›› Issue (3): 71-74.

• 生物技术 • 上一篇    下一篇

环介导等温扩增技术及其在动物疫病检测中的应用

李结, 王培园, 张萍, 李金平, 蔺智宾, 李国清   

  1. 华南农业大学兽医学院,广东广州 510642
  • 收稿日期:2011-09-08 修回日期:1900-01-01 出版日期:2012-03-20 发布日期:2012-03-20
  • 通讯作者: 李国清

Loop-Mediated Isothermal Amplification Technique and Its Application in Detection of Animal Disease

LI Jie, WANG Pei-yuan, ZHANG Ping, LI Jin-ping, LIN Zhi-bin, LI Guo-qing   

  1. College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China
  • Received:2011-09-08 Revised:1900-01-01 Online:2012-03-20 Published:2012-03-20

摘要: 环介导等温扩增技术(LAMP)是一种新型的体外等温扩增特异核酸片段的技术,具有灵敏度高、特异性强及快速检测等优点,现已经广泛应用于动物疫病的快速检测。文章对LAMP的反应原理、技术特点及其近年来在动物疫病检测中的应用作一综述,旨在为该技术的深入研究和应用提供参考。

关键词: 环介导等温扩增; 动物疫病; 检测

Abstract: Loop-mediated isothermal amplification(LAMP) technique is a sensitive,specific method which has been widely used for rapid detection of animal disease, this article reviewed LAMP amplification mechanism, technical characteristics and its application in recent years, aiming to provide reference in further research and application of LAMP.

Key words: loop-mediated isothermal amplification; animal disease; detections

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