生长抑素基因第1外显子多态性与湘西黄牛体尺性状的关联分析

›› 2012, Vol. 39 ›› Issue (11): 165-168.

生长抑素基因第1外显子多态性与湘西黄牛体尺性状的关联分析

罗仍卓么^1,2, 王兴平^1,2, 李峰^1,2, 王京仁^1,2, 李娜^1,2

1. 湖南文理学院生命科学学院,湖南常德 415000;
2. 湖南省高校动物学重点实验室,湖南常德 415000

收稿日期:2012-04-06 出版日期:2012-11-20 发布日期:2012-11-22
通讯作者: 王兴平(1978-),男,陕西人,博士,副教授,研究方向:动物基因表达调控与分子育种。E-mail: nwsuafwxp@yahoo.com.cn E-mail:nwsuafwxp@yahoo.com.cn
作者简介:罗仍卓么(1978-),女,青海人,硕士,研究方向:动物基因表达调控与分子育种。
基金资助:
湖南省科技计划项目(2010NK3043);湖南省自然科学基金项目(11JJ4020);湖南文理学院博士启动项目(BSQD1002);湖南省高校创新平台开放基金项目(11K045);湖南省动物学重点学科建设项目。

Polymorphisms of Exon 1 of SST Gene and its Associations with Growth Traits in Xiangxi Cattle

LUOREN Zhuo-ma^1,2, WANG Xing-ping^1,2, LI Feng^1,2, WANG Jin-ren^1,2, LI Na^1,2

1. College of Life Science, Hunan University of Arts and Science, Changde 415000,China;
2. Key Laboratory of Zoology in Hunan Higher Education, Changde 415000,China

Received:2012-04-06 Online:2012-11-20 Published:2012-11-22

摘要/Abstract

摘要： 为探讨湘西黄牛分子遗传特征和寻找生长性状相关的分子标记,本试验采用PCR-SSCP方法检测湘西黄牛生长抑素(somatostatin,SST)基因第1外显子126 bp处g.934G>A位点的多态性,并进行了多态性与生长性状的关联分析。结果表明,湘西黄牛在g.934G>A位点有G和A两个等位基因,G等位基因占优势,检测到GG、AG和AA 3种基因型,呈中度多态,达到Hardy-Weinberg平衡状态(P>0.05)。湘西黄牛GG基因型个体的体高和体长显著大于AA基因型个体(P<0.05);GG和AG基因型个体的胸围和体重极显著大于AA基因型个体(P<0.01)。G等位基因对湘西黄牛的体高、体长、胸围和体重4个性状均为正效应。本试验结果提示,SST基因g.934G>A位点可能为湘西黄牛生长性状的分子遗传标记位点。

关键词: 湘西黄牛; SST基因; 多态性; PCR-SSCP; 生长性状

Abstract: In order to study molecular genetic characters of Xiangxi cattle and search molecular markers related growth traits of Xiangxi cattle, the polymorphism of bovine SST gene was detected by PCR-SSCP, and association between polymorphism of g.934G>A locus in exon 1 of somatostatin (SST) gene and growth traits of Xiangxi cattle were analyzed. The results showed that there were two alleles (G and A) and three genotypes (GG, AG and AA) on g.934G>A locus of SST gene in Xiangxi cattle, which G allele frequency was more than that of A allele. The locus of g.934G>A was in moderate polymorphism, and fitted in Hardy-Weinberg equilibrium (P>0.05). The individuals with GG genotype had larger withers heigh and body length than AA genotype (P<0.05), and the heart girth and body weight of individuals with GG and AG genotypes were significantly larger than AA genotype(P<0.01). G allele had positively effect on withers heigh, body length, heart girth and body weight of Xiangxi cattle. The results of this study suggested that g.934G>A of exon 1 in SST gene might be effect on growth traits of Xiangxi cattle, and it might be used as the molecular genetic marker for breeding.

Key words: Xiangxi cattle; SST gene; polymorphism; PCR-SSCP; growth traits

中图分类号:

S813.3

罗仍卓么, 王兴平, 李峰, 王京仁, 李娜. 生长抑素基因第1外显子多态性与湘西黄牛体尺性状的关联分析[J]. , 2012, 39(11): 165-168.

LUOREN Zhuo-ma, WANG Xing-ping, LI Feng, WANG Jin-ren, LI Na. Polymorphisms of Exon 1 of SST Gene and its Associations with Growth Traits in Xiangxi Cattle[J]. China Animal Husbandry & Veterinary Medicine, 2012, 39(11): 165-168.

参考文献

1 王子荣,陈伟华,陈杰.生长抑素基因工程苗和半胱胺对放牧羔羊增重效果的影响[J].畜牧与兽医,2001,33(1): 3~5.
2 刘桂琼, 曹勤忠, 姜勋平, 等. 小梅山猪促卵泡素β基因多态性及其与繁殖性能的关系[J]. 中国畜牧杂志, 2004, 40(12): 6~8.
3 赵永安,马尔玉,张佩君,等.生长抑素基因工程疫苗免疫牦牛绵羊试验[J].四川畜牧兽医,2004,31(5): 31~33.
4 赵雯,姜丽花,何晓波,等. 13/17罗伯逊易位猪生长抑素基因的多态性研究[J]. 中国畜牧兽医, 2009, 36(1): 53~56.
5 高丽,昝林森,薛梅,等.SST基因多态性及其与牛生长性状的关联分析[J]. 西北农林科技大学学报(自然科学版),2011,39(11):65~69.
6 Brazeau P, Vale W, Burgus R, et al. Hypothalamic polyeptide that inhibits the secretion of immunoreactive pituitary growth hormone[J]. Science, 1973, 179(68): 77~79.
7 Fadlalla A M, Spencer G S, Lister D. The effect on passive immunization against somatostatin on marker retention time in lambs [J]. J Anm Sci, 1985, 61(1): 234~238.
8 Gao L, Zan L S, Wang H B, et al. Polymorphism of somatostatin gene and its association with growth traits in Chinese cattle[J].Genet Mol Res,2011,10(2):703~711.
9 Giustina A, Veldhuis J D. Pathophysiology of the neuroregulation of growth hormone secretion in experimental animals and the human[J]. Endocrine Reviews, 1998, 19(6): 717~797.
10 Jin Q J, Sun J J, Fang X T, et al. Molecular characterization and polymorphisms of the caprine somatostatin (SST) and SST receptor 1 (SSTR1) genes that are linked with growth traits[J]. Mol Biol Rep, 2011,38(5): 3129~3135.
11 Komatsu M, Kojima M, Okamura H, et al. Age-related changes in gene expression of the growth hormone secret agogue and growth hormone-releasing hormone receptors in Holstein-Friesian cattle[J]. Domest Anim Endocrinol, 2012, 42(2):83~93.
12 Koyama M, Yin C, Ishii H, et al. Somatostatin inhibition of GnRH neuronal nctivity and the morphological relationship between GnRH and somatostatin neurons in rats endocrinology[J]. Endocrinology,2012, 153(2):806~814.
13 Krantic S. Peptides as regulators of the immune system: Emphasis on somatostatin[J]. Peptides, 2000, 21(12): 1941~1964.
14 Lawrence M E, Schelling G T, Byers F M, et al. Improvement of growth and feed efficiency in cattle by active immunization against somatostatin[J]. J Anim Sci, 1986, 63(suppl): 215~224.
15 McClure M C, Morsci N S, Schnabel R D, et al. A genome scan for quantitative trait loci influencing carcass, post-natal growth and reproductive traits in commercial Angus cattle. Anim Genet, 2010, 41(6):597~607.
16 Morsci N S, Schnabel R D, Taylor J F. Association analysis of adiponectin and somatostain polymorphism on BTA1 with growth and carcass traits in Angus cattle[J]. Anim Genet, 2006, 37(6): 554~562.
17 Plotsky P M, Vale W. Patterns of growth hormone-releasing factor and somatostatin secretion into the hypophysial-portal circulation of the rat [J]. Science, 1985, 230(4724): 461~463.
18 van Kessel A G, Laarveld B. Effect of passive immunization against somatostatin using a high-affinity antiserum on growth, IGF-Ⅰ and thyroid hormone levels in neonatal lambs reared under warm or cold environmental conditions[J]. Can J Anim Sci, 1994, 74: 23~27.

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