关键词: 猪; H3.3基因; 实时荧光定量PCR; 正反交; 基因表达; 发育变化

Abstract: To understand the developmental rules of histone variant H3.3 gene expression of pigs, we detected the expressional level of swine muscle issue under different ages and reciprocal cross. The rusults showed that the H3.3 gene were expressed in longissimus muscle in different periods after birth and the expression substantially growed in number with increasing age. There was difference between reciprocal cross pigs: anti-cross (Erhualian♂×Yorkshire♀) group of one-day age expressed greater than the orthogonal (Yorkshire♂×Erhualian♀) group;expression of the orthogonal group were higher than the anti-cross group after 20 days, while there was no significant difference in expression of orthogonal group and anti-cross group uder the same period. Our study confirmed histone variant H3.3 incoorporate in the swine muscle issue and obtain developmental rules of H3.3 expression in muscle cells and to further study the replacement of histione variants on eukaryotic cell growth and development-related gene regulation and modification of basis for the study of molecular mechanisms.

Key words: pig; H3.3 gene; Real-time quantitative PCR; hybrids and reciprocal; gene expression; developmental rules