›› 2010, Vol. 37 ›› Issue (5): 137-139.

• 遗传繁育 • 上一篇    下一篇

藏猪肝X受体(LXRs)基因PCR-RFLP多态性分析

李宝玉1,强巴央宗2,王强3,张明明3,辜雪冬2,凌遥1,张浩1   

  1. (1.中国农业大学动物科技学院, 北京 100193; 2.西藏农牧学院动物科学系, 林芝 860000;3.安徽省农业科学院情报研究所, 合肥 230031)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-05-20 发布日期:2010-05-20
  • 通讯作者: 张浩

PCR-RFLP Polymorphisms of LXRs Genes in Tibet Pigs

LI Bao-yu1,CHAMBA Yang-zom2,WANG Qiang3,ZHANG Ming-ming3,GU Xue-dong2,LING Yao1,ZHANG Hao1   

  1. (1.College of Animal Science and Technology, China Agricultural University,Bejing 100193,China;2.Department of Animal Science, College of Agriculture and Animal Husbandry,Linzhi 860000,China;3.Institute of Informatics, Anhui Academy of Agricultural Sciences, Hefei 230031, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-05-20 Published:2010-05-20
  • Contact: ZHANG Hao

摘要: LXRα和LXRβ基因是猪肌肉发育和脂肪沉积的重要候选基因。本研究利用PCR-RFLP技术测定了112头藏猪的LXRα-Bsl I和LXRβ-Aci I多态性。结果显示,藏猪群体中LXRα-Bsl I有CC、CG和GG 3种基因型,基因型频率分别为0.4286、0.4643和0.1071;LXRβ-Aci I有TT、TC和CC 3种基因型,基因型频率分别为0.1964、0.5179和0.2857;与瘦肉型猪基因型分布差别较大。推测可能与藏猪肌肉和脂肪发育遗传特点有关。

关键词: 藏猪; LXRα基因; LXRβ基因; PCR-RFLP; 多态性

Abstract: The LXRα and LXRβ genes were main candidate genes for muscle growth and fat deposition. In the present study, the polymorphisms of LXRα-Bsl I and LXRβ-Aci I were measured with PCR-RFLP in 112 individuals of Tibet pig. The results showed that the LXRα-Bsl I in Tibet pig had 3 genotypes of CC, CG and GG, and frequents were 0.4286, 0.4643 and 0.1071 respectively. The LXRβ-Aci I in Tibet pig had 3 genotypes of TT, TC and CC, and frequents were 0.1964, 0.5179 and 0.2857 respectively. For the genotype distribution of the two genes Tibet pig was much different with lean-type pig breeds, which might be related to the special traits of muscle and fat developments in Tibet pig.

Key words: Tibet pig; LXRα gene; LXRβ gene; PCR-RFLP; polymorphism

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