›› 2010, Vol. 37 ›› Issue (5): 126-128.

• 遗传繁育 • 上一篇    下一篇

京海黄鸡连续3个世代类胰岛素样生长因子-Ⅰ外显子3的多态性研究

王慧华1,王金玉1,李源1,侯启瑞1,张跟喜1,施会强2   

  1. (1.扬州大学动物科学与技术学院, 扬州 225009; 2.江苏京海集团, 南通 226103)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-05-20 发布日期:2010-05-20
  • 通讯作者: 王金玉

Study on the Polymorphism of Exon 3 in Insulin-like Growth Factor-Ⅰ Gene in Three Generations of Jinghai Yellow Chicken

WANG Hui-hua1,WANG Jin-yu1,LI Yuan1,HOU Qi-rui1, ZHANG Gen-xi1,SHI Hui-qiang2   

  1. (1.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;2.Jiangsu Jinghai Poultry Group Co. Ltd., Nantong 226103, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-05-20 Published:2010-05-20
  • Contact: WANG Jin-yu

摘要: 以7、8、9世代京海黄鸡母鸡为试验材料,采用PCR-SSCP技术对IGF-Ⅰ基因外显子3多态位点进行研究,并计算基因型、基因频率、卡方值和部分遗传多态性指标。结果表明,A等位基因随着世代数的增加不断减少;3个世代之间基因型分布达到显著(P<0.05)或极显著(P<0.01)差异;遗传杂合度随世代数的增加而升高;该位点3个世代均为中度多态。初步推断选留的确对IGF-Ⅰ基因31位点造成了影响,该位点可应用于育种实践中。

关键词: 京海黄鸡; 类胰岛素样生长因子-Ⅰ; SNP位点; 多态性

Abstract:

Genetic diversity of exon 3 in insulin-like growth factor-Ⅰ(IGF-Ⅰ) gene was studied using PCR-SSCP technique in seven, eight and nine generations of Jinghai yellow chicken population in the present study, and calculated the genotype, gene frequency, chi-square value and some indicators of genetic polymorphism. The results showed that alleles increased with decreasing the number of generations; genotype distribution between the three generations achieved a significant(P<0.05) or very significant(P<0.01) difference; heterozygosis increased with the number of generations increased; the locus polymorphism of three generations were moderate. Indeed, the initial inference elected to stay on the IGF-Ⅰ gene had an impact on 31 points, the loci could be applied in breeding practice.

Key words: Jinghai yellow chicken; insulin-like growth factor-Ⅰ; SNP; polymorphisms

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