›› 2010, Vol. 37 ›› Issue (4): 118-120.

• 生物技术 • 上一篇    下一篇

欧拉型藏绵羊血清运铁蛋白多态性的研究

信金伟   

  1. (西藏自治区农牧科学院畜牧兽医研究所, 拉萨 850009)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-04-20 发布日期:2010-04-20

Investigation on Polymorphism of Serum Transferrin in Oura Tibetan Sheep

XIN Jin-wei   

  1. (Institute of Academy and Animal Science, Tibet Autonomous Academy of Agricultural and Animal Sciences, Lhasa 850009, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-04-20 Published:2010-04-20

摘要: 采用聚丙烯酰胺凝胶电泳方法对欧拉型藏绵羊的74份血样进行了血清运铁蛋白(serum transferrin,TF)多态性的研究。结果发现,被检欧拉型藏绵羊的血清TF位点上检测出TFA、TFB、TFC、TFD和TFM 5个复等位基因,其中TFB、TFC和TFD为优势等位基因,其基因频率分别为0.4122、0.2973和0.2162;发现TFAB、TFAC、TFAD、TFBB、TFBC、TFBD、TFBM、TFCC和TFDD等9种基因型,其中TFBC、TFBB、TFBD、TFCC和TFDD为优势基因型,其基因型频率分别为0.2703、0.1622、0.1622、0.1351和0.1216;TF位点的基因杂合度为0.6921。

关键词: 欧拉型藏绵羊; 血清运铁蛋白; 多态性

Abstract: The polymorphism of the serum transferrin was investigated in the blood samples from 74 Oura Tibetan sheep by means of PAGE. The results were as follows: five alleles including TFA,TFB,TFC,TFD and TFM were showed in total on the locus of serum transferrin in the examined Oura Tibetan sheep, and of them the TFB,TFC and TFD were the preponderant alleles, and their frequencies were 0.4122,0.2973 and 0.2162. Nine genotypes including TFAB,TFAC,TFAD,TFBB,TFBC,TFBD,TFBM,TFCC and TFDD were showed, of them TFBC,TFBB,TFBD,TFCC and TFDD were the preponderant genotypes, and their frequencies were 0.2703,0.1622,0.1622,0.1351 and 0.1216. The gene heterozygosity on the locus of serum transferrin was 0.6921.

Key words: Oura Tibetan sheep; serum transferrin; polymorphism

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