›› 2010, Vol. 37 ›› Issue (2): 111-114.

• 遗传繁育 • 上一篇    下一篇

马eCG/LHβ亚基基因多态性研究

廉嵩1, 鲍海港1,2, 张浩1,2, 赵春江1,2, 连正兴1, 韩国才1,2   

  1. (1.中国农业大学动物科技学院, 北京 100193; 2.中国农业大学马研究中心, 北京 100193)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-02-20 发布日期:2010-02-20

Study on Polymorphisms of eCG/LHβ Subunit Gene

LIAN Song1, BAO Hai-gang1,2, ZHANG Hao1,2, ZHAO Chun-jiang1,2,
LIAN Zheng-xing1, HAN Guo-cai1,2
  

  1. (1.Institute of Animal Science and Technology, China Agricultural University, Beijing 100193, China;2.Equine Center, China Agricultural University, Beijing 100193, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-02-20 Published:2010-02-20

摘要: 摘要: 马绒毛膜促性腺激素(equine chorionic gonadotropin,eCG)是由妊娠期母马胎儿尿膜—绒毛膜细胞分泌的一种由α、β两个亚基组成的异源二聚体糖蛋白,具有FSH、LH的双重活性。采用PCR-SSCP和测序方法对28匹蒙古马和25匹纯血马的eCG/LHβ亚基基因的多态性进行研究,结果发现,在蒙古马eCG/LHβ亚基基因5′调控区存在一突变位点:G-415A分为AA、BB、AB 3种基因型,AA、BB、AB基因型频率分别为0.89、0.07、0.04;纯血马此基因中未检测到多态性。

关键词: 关键词:eCG/LHβ亚基基因; 多态性; SSCP

Abstract: Abstract: 28 Mongolia mares and 25 Thoroughbred mares were subjected to SNP analysis by PCR-SSCP and sequencing, one SNPs was found in 5′flanking region of eCG/LHβ gene, the mutation G-415A was found in Mongolia mares but not in Thoroughbred mares, the frequent of three genotypes:AA, BB, AB are 0.89, 0.07, 0.04, respectively.

Key words: Key words: eCG/LHβ subunit gene; polymorphisms; SSCP