›› 2010, Vol. 37 ›› Issue (12): 210-211.

• 兽医临床 • 上一篇    下一篇

小鹅瘟的PCR诊断及病理观察

葛晨霞,王龙涛,张加力,马磊,胡桂学   

  1. (吉林农业大学动物科学技术学院,长春 130118)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-12-20 发布日期:2010-12-20
  • 通讯作者: 胡桂学

The PCR Diagnosis and Pathological Observation of Gosling Plague

GE Chen-xia, WANG Long-tao, ZHANG Jia-li, MA Lei, HU Gui-xue   

  1. (College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-12-20 Published:2010-12-20
  • Contact: HU Gui-xue

摘要: 取长春某鹅场2份疑似小鹅瘟感染病死鹅的肝脏组织进行PCR检测,结果均扩增出与预想结果一致的375 bp特异片段。取病死鹅肺脏、脾脏、肠道等组织,用10%中性福尔马林固定,石蜡包埋,HE染色,进行病理组织学观察,结果显示,肠道呈纤维素性-坏死性肠炎病变,肺脏、肝脏、肾脏、脑等器官实质细胞均出现不同程度的损伤。

关键词: 小鹅瘟; PCR; 诊断; 病理观察

Abstract: PCR test for livers of 2 dead geese that suspected goose parvovirus infection, amplified 375 bp specific bangs which agreed with the desired results. The organization, such as lung, spleen and intestinal canal, were taken into 10% formaldehyde solution, paraffin embedding, HE dyeing and histologic analysis. The results showed that the intestinal in cellulose-enteritis lesions; the substantial cells in lungs, liver, kidney and brain were in different degree of damage.

Key words: Gosling plague; PCR; diagnosis; pathological observation

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