›› 2010, Vol. 37 ›› Issue (11): 153-155.

• 疾病防治 • 上一篇    下一篇

92株猪腹泻大肠杆菌的耐药性分析

张俊丰1,2,陈琳1,魏冬霞1,黄东璋1,付文贵2,翟少钦2,周淑兰2   

  1. (1.江苏畜牧兽医职业技术学院,泰州 225300;2.重庆市畜牧科学研究院,重庆 402460)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-11-20 发布日期:2010-11-20
  • 通讯作者: 陈琳

Analysis of Resistance for 92 Diarrheic Escherichia coli Isolates from Pig Farms

ZHANG Jun-feng1,2, CHEN Lin1, WEI Dong-xia1, HUANG Dong-zhang1, FU Wen-gui2,ZHAI Shao-qin2,ZHOU Shu-lan2   

  1. (1.Jiangsu Animal Husbandry and Veterinary College, Taizhou 225300, China;2.Chongqing Animal Science Institution, Chongqing 402460, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-11-20 Published:2010-11-20
  • Contact: CHEN Lin

摘要: 分析92株猪腹泻大肠杆菌的耐药性,为临床合理用药提供理论依据。采集腹泻病猪直肠拭子,分离、鉴定大肠杆菌,采用微量稀释法测定分离菌对17种抗菌药物的敏感性。结果表明,从92份腹泻直肠拭子样品中共分离鉴定出92株大肠杆菌,对17种抗菌药物的耐药率从高到低依次为磺胺甲噁唑(100%)、甲氧苄啶(100%)、卡那霉素(89%)、庆大霉素(80%)、氯霉素(78%)、链霉素(76%)、新霉素(76%)、四环素(73%)、大观霉素(72%)、恩诺沙星(66%)、氨苄西林(50%)、阿米卡星(45%)、氟苯尼考(32%)、安普霉素(27%)、利福平(23%)、头孢唑啉(22%)、头孢噻呋(0%);所有菌株均为多重耐药菌株,92株菌的耐药谱型为81种,菌株最多对14种药物耐药,最少对3种药物耐药。研究结果表明,92株猪腹泻大肠杆菌耐药谱广,耐药率高。

关键词: 猪; 大肠杆菌; 耐药; 腹泻

Abstract: The resistance Escherichia coli isolates from 92 diarrheic pig rectal swab samples were analyzed to 17 antibacterial agents for the rational using principle. Total of 92 diarrheic pig rectal swab samples were collected to isolate and identify Escherichia coli.Then all isolates were tested for susceptibility to 17 antimicrobial agents by the broth microdilution method. 92 Escherichia coli isolates recovered from diarrheic pigs were identified. And their resistant ratio were orderd as sulfamethoxazole(100%),trimethoprim(100%), kanamycin(89%),gentamicin(80%), chloramphenicol(78%), streptomycin(76%),neomycin(76%), tetracycline(73%), spectinomycin(72%), enrofloxacin(66%), ampicillin(50%), amikacin(45%), florfenicol(32%), apramycin(27%), rifampicin(23%), cefazolin(22%), ceftiofur(0%). All isolates were multiple resistant isolates and 81 types of resistant spectrum were presented in 92 Escherichia coli isolates. These Escherichia coli isolates were resistant against 14 antibacterial agents at most, and 3 antibacterial agents at least. Therefore wide resistant spectrum and high resistant ratio were presented in the 92 diarrheic pig Escherichia coli isolates.

Key words: pig; Escherichia coli; resistance; diarrhea

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