›› 2007, Vol. 34 ›› Issue (5): 54-56.

• 遗传繁育 • 上一篇    下一篇

肉用绵羊2号染色体GDF8区域4种微卫星标记多态性研究

宋彦红1,石国庆2,柳楠1,代蓉2,任航行2,刘国庆3
  

  1. 1. 莱阳农学院动物科技学院,青岛 266109;2.新疆兵团绵羊繁育生物技术重点实验室,石河子 832000;3.合肥工业大学生物与食品工程学院,合肥 230009
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-05-20 发布日期:2007-05-20

Study on Four Microsatellite DNA Polymorphism in the Region of GDF8 on Chromosome 2 in Meat Sheep Populations

SONG Yanhong1,SHI Guoqing2, LIU Nan1,DAI Rong2,REN Hangxing2, LIU Guoqing3
  

  1. 1.College of Animal Science and Technology, Laiyang Agricultural University, Qingdao 266109,China; 2.Key Laboratory of Sheep Reproduction Biology of Xinjiang Bingtuan, Shihezi 832000,China; 3. School of Biotechnology and Food Engineering,Hefei University of Technology, Hefei 230009,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-05-20 Published:2007-05-20

摘要: 选择了2号染色体GDF8区域可能与生产性能相关性较大的4个高度多态微卫星基因座 (BM81124,TEXAN2,FCB128,BMS1591),分析这些位点在 Dorset、Suffolk和Texel 3个肉羊品种的多态分布情况。结果表明,这4个微卫星标记在3个绵羊品种中的等位基因数分别为:10、12、7、11,其多态信息含量/杂合度分别为:0.7778/0.8045、0.712/0.7520、0.7551/0.7852、0.8057/0.8289,可见这些微卫星位点在3个绵羊品种中具有比较丰富的多态性。因此它们可以作为进行生产性状与标记间连锁分析的遗传标记,用于进一步的QTL定位研究。

关键词: 肉用绵羊; 微卫星标记; 多态性; GDF8

Abstract: According to report, four highly polymorphic microsatellite loci possibly linked to production traits and mapped to the region of GDF8 on sheep chromosome 2 were selected. they were BM81124,TEXAN2,FCB128,BMS1591. They were analyzed for polymorphism in Dorset,Suffolk and Texel meat sheep populations. Results showed that the number of alleles were 10,12,7,11 and polymorphic information content / Heterozygosity were 0.7778/0.8045,0.712/0.7520,0.7551/0.7852,0.8057/0.8289.We can see these microsatellite loci have rich polymorphism in three groups.So they can be used as the linkage analyse between the production traits and makers,and used for further location of QTL.

Key words: meat sheep; microsatellite DNA; polymorphism; GDF8

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