›› 2007, Vol. 34 ›› Issue (5): 103-104.

• 疾病防治 • 上一篇    下一篇

传染性支气管炎病毒M41和Ma5的气管环培养特性研究

刘伟,何颖,陈忠伟   

  1. 广西兽医研究所,南宁 530001
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-05-20 发布日期:2007-05-20

Comparative Characterstic of Infectious Bronchitis Virus M41 and Ma5 Cultured in Tracheal Organ

LIU Wei,HE Ying,CHEN Zhongwei
  

  1. Veterinary Research Institute of Guangxi, Nanning 530001, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-05-20 Published:2007-05-20

摘要: 本试验以鸡胚气管环纤毛运动作为指示系统,研究传染性支气管炎病毒IBV-M41和IBVMa5的气管环培养特性。用100 TOC-ID50病毒量的两毒株分别感染鸡胚气管环培养物,观察感染后不同时间气管纤毛的存活率。结果表明,IBV-M41毒株致死气管环上皮细胞的速度要比毒株IBV-Ma5快,说明2个毒株的培养特性与毒力一致。

关键词: 气管环培养; 传染性支气管炎; 培养特性

Abstract: The characteristic of IBV-M41 and IBV-Ma5 strains cultured in tracheal organ was assessed by the ciliary activity of chicken embryo tracheal organ. After the virus (containing 100TOCID50) was inoculated into trachealring organ cultures, the survival rates of cilia were assessed . The results showed that the survival rates of epithelial cells inoculated with IBV-Ma5 was high than with IBV-M41, which means their culture characteristics accord with their toxicity.

Key words: tracheal organ cultures(TOC); infectious bronchitis; characteristic of cultrues

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