2株重组禽传染性支气管炎病毒的分离鉴定及基因组序列分析

doi:10.16431/j.cnki.1671-7236.2024.09.024

摘要/Abstract

摘要： 【目的】了解禽传染性支气管炎病毒(Infectious bronchitis virus,IBV)的遗传变异情况。【方法】采集山东地区部分养殖场中疑似IBV感染鸡病料组织，接种SPF鸡胚进行病毒分离鉴定，利用二代测序技术进行全基因组测序鉴定，并基于全基因组序列进行遗传进化和关键氨基酸位点分析。【结果】感染鸡剖检可见气管环出血，管腔内有黄色或黑黄色栓塞物等。从病料中共分离获得2株IBV，分别命名为SDJN和SDLY。相似性分析结果显示，分离株SDJN和SDLY与GⅠ-19基因型参考毒株相似性较高，核苷酸序列相似性为96.0%～97.3%，氨基酸序列相似性为94.9%～100%。氨基酸序列分析结果显示，2株IBV分离株与其他毒株相比存在多个氨基酸变异位点。系统进化树结果显示，分离株SDJN和SDLY与IBV GⅠ-19基因型参考毒株位于同一分支。重组分析结果显示，分离株SDJN和SDLY为多种基因型毒株的重组毒株，均以ck/CH/LDL/091022株为主要亲本毒株。【结论】本研究从山东地区分离得到2株IBV，2株分离株与GⅠ-19基因型毒株密切相关，是一种新型重组IBV毒株。研究结果为进一步研究IBV流行与进化及禽传染性支气管炎的防控奠定基础。

关键词: 禽传染性支气管炎病毒; 分离; 鉴定; 全基因组; 遗传进化分析

Abstract: 【Objective】 The experiment was aimed to understand the genetic variation of avian Infectious bronchitis virus (IBV). 【Method】 Samples suspected to be infected with IBV was collected from partial farms in Shandong, inoculated with SPF chicken embryos for virus isolation and identification.Whole genome sequencing was performed using second-generation sequencing technology, and genetic evolution and key amino acid sites were analyzed based on the whole genome sequence. 【Result】 Autopsy of infected chickens showed hemorrhages in the tracheal rings and yellow or black-yellow embolism in the lumen.Two strains of IBV were isolated from the samples, and named SDJN and SDLY, respectively.Similarity analysis showed that isolates SDJN and SDLY had high similarity with GⅠ-19 genotype reference strains, with nucleotide similarity of 96.0%-97.3% and amino acid similarity of 94.9%-100%.Amino acid sequence analysis showed that the two IBV isolates had multiple amino acid variation sites compared with other strains.Phylogenetic tree results showed that isolates SDJN and SDLY were located in the same branch as the reference strains of the IBV GⅠ-19 genotype.Recombinant analysis showed that isolates SDJN and SDLY were recombinant strains of multiple genotypic strains, both with the ck/CH/LDL/091022 strain as the main parental strain. 【Conclusion】 In this study, two IBV isolates were isolated from Shandong province.The two isolates were closely related to GⅠ-19 genotype, and were a new recombinant IBV strain.The results laid a foundation for further study on the prevalence and evolution of IBV and the prevention and control of avian infectious bronchitis.

Key words: avain Infectious bronchitis virus; isolation; identification; whole genome; genetic evolutionary analysis

中图分类号:

S855.3

李峰, 谢宾, 张帅. 2株重组禽传染性支气管炎病毒的分离鉴定及基因组序列分析[J]. 中国畜牧兽医, 2024, 51(9): 3958-3969.

LI Feng, XIE Bin, ZHANG Shuai. Isolation，Identification and Genome Sequence Analysis of Two Recombinant Strains of Avian Infectious Bronchitis Virus[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(9): 3958-3969.

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