鸡传染性支气管炎病毒广西株M基因的克隆与序列分析

doi:10.16431/j.cnki.1671-7236.2016.02.013

›› 2016, Vol. 43 ›› Issue (2): 377-382.doi: 10.16431/j.cnki.1671-7236.2016.02.013

鸡传染性支气管炎病毒广西株M基因的克隆与序列分析

屈素洁, 施开创, 邹联斌, 胡杰, 莫胜兰, 尹彦文, 张步娴, 李军, 梁媛

广西区动物疫病预防控制中心, 南宁 530001

收稿日期:2015-06-12 出版日期:2016-02-20 发布日期:2016-03-02
通讯作者: 梁媛 E-mail:649921923@126.com
作者简介:屈素洁(1982-),女,广西南宁人,高级兽医师,研究方向:畜禽传染病学及分子病毒学,E-mail:sujie2008abc@163.com
基金资助:
广西区水产畜牧兽医局科技项目(桂渔牧科1204935)

Cloning and Sequence Analysis of M Gene of Avian Infectious Bronchitis Virus Guangxi Strain

QU Su-jie, SHI Kai-chuang, ZOU Lian-bin, HU Jie, MO Sheng-lan, YIN Yan-wen, ZHANG Bu-xian, LI Jun, LIANG Yuan

Guangxi Center for Animal Disease Control and Prevention, Nanning 530001, China

Received:2015-06-12 Online:2016-02-20 Published:2016-03-02

摘要/Abstract

摘要： 参照GenBank中鸡传染性支气管炎病毒(IBV)的核苷酸序列设计1对引物,利用 PCR 扩增IBV广西株的M基因片段,将其克隆到pMD18-T载体中.序列分析结果表明,M基因全长为678 bp,编码225个氨基酸,近N端含有2个潜在的N-糖基化位点,3个跨膜区位于23—98肽段区,亲水区较疏水区更易变异.IBV广西株与国内外IBV参考毒株相比,核苷酸序列同源性为83.6%~92.5%,氨基酸序列同源性为82.7%~95.1%.系统进化分析结果显示IBV广西株与SAIB20和LX4两参考株位于同一个分支上,它们的亲缘关系较近,而与其他参考株属于不同的分支,亲缘关系较远.结果表明IBV广西株是1株新的IBV变异株.

关键词: 鸡传染性支气管炎病毒; M基因; 基因克隆; 序列分析

Abstract: According to the M gene nucleotide sequence of avian infectious bronchitis virus (IBV) published in GenBank,one pair of primers were designed,the M gene fragments of IBV isolated from Guangxi province were amplified by PCR.Then the amplified fragments were cloned into pMD18-T vector and the positive recombinant plasmids were sequenced.The results showed that M gene from all of the IBV isolates consisted of 678 bp,coding for 225 amino acids.Two glycosylated sites were located nearby the N-terminal,three transmembrane domains were located in the 23 to 98 peptide region.Variations within the hydrophilicity region were easier than that in the hydrophobicity region.Compared with that of other published IBV strains,the homologies of nucleotide and amino acid sequences of the isolates were 83.6% to 92.5% and 82.7% to 95.1%,respectively.The phylogenetic tree analysis showed that it was closely related to SAIB20 and LX4,and clustered into one group;But it belonged to different branches with other reference strains,and had a distant relationship.These results suggested that the isolate was a new variant of IBV.

Key words: avian infectious bronchitis virus; M gene; gene clone; sequence analysis

中图分类号:

屈素洁, 施开创, 邹联斌, 胡杰, 莫胜兰, 尹彦文, 张步娴, 李军, 梁媛. 鸡传染性支气管炎病毒广西株M基因的克隆与序列分析[J]. , 2016, 43(2): 377-382.

QU Su-jie, SHI Kai-chuang, ZOU Lian-bin, HU Jie, MO Sheng-lan, YIN Yan-wen, ZHANG Bu-xian, LI Jun, LIANG Yuan. Cloning and Sequence Analysis of M Gene of Avian Infectious Bronchitis Virus Guangxi Strain[J]. , 2016, 43(2): 377-382.

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鸡传染性支气管炎病毒广西株M基因的克隆与序列分析

Cloning and Sequence Analysis of M Gene of Avian Infectious Bronchitis Virus Guangxi Strain

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