两株鸡传染性支气管炎病毒的分离鉴定及S1基因序列分析

doi:10.16431/j.cnki.1671-7236.2015.08.007

›› 2015, Vol. 42 ›› Issue (8): 1963-1972.doi: 10.16431/j.cnki.1671-7236.2015.08.007

两株鸡传染性支气管炎病毒的分离鉴定及S1基因序列分析

尚辉琴¹, 李琳¹, 陈瑞爱^1,2, 刘红波¹, 梁梅兰¹, 贺东生^1,2

1. 广东大华农动物保健品股份有限公司, 云浮 527400;
2. 华南农业大学兽医学院, 广州 510642

收稿日期:2015-03-09 出版日期:2015-08-20 发布日期:2015-08-27
通讯作者: 贺东生 E-mail:dhe@scau.edu.cn
作者简介:尚辉琴(1985-),女,江西丰城人,硕士,研究方向:预防兽医学,E-mail:106087098@qq.com;李琳(1979-),女,广东梅州人,学士,研究方向:预防兽医学,E-mail:mulian9618905@qq.com
基金资助:
广东大华农动物保健品股份有限公司科研项目(03-63C201206-4)

Isolation,Identification and Sequence Analysis of S1 Gene of Two Strains of Avian Infectious Bronchitis Viruses

SHANG Hui-qin¹, LI Lin¹, CHEN Rui-ai^1,2, LIU Hong-bo¹, LIANG Mei-lan¹, HE Dong-sheng^1,2

1. Guangdong Dahuanong Animal Health Products Co., Ltd., Yunfu 527400, China;
2. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China

Received:2015-03-09 Online:2015-08-20 Published:2015-08-27

摘要/Abstract

摘要： 为调查广东地区鸡传染性支气管炎病毒(IBV)的流行及其遗传变异情况,本研究通过病料SPF鸡胚接种和鸡胚尿囊液的RT-PCR鉴定,于2013年从广东湛江地区不同发病鸡场分离到两株IBV,分别命名为CK/CH/GD/ZJ10/2013和CK/CH/GD/ZJ11/2013,并对这两株IBV的S1基因进行序列分析。结果显示,CK/CH/GD/ZJ10/2013株S1基因全长1 626 bp,编码542个氨基酸,其裂解位点为NRFRR,属于基因型Ⅲ,并推测其为基因型Ⅲ毒株(CK/CH/GX/NN11-3)与基因型Ⅰ毒株(GX-NN-6)在S1基因处发生重组而产生的新毒株;CK/CH/GD/ZJ11/2013株S1基因全长1620 bp,编码540个氨基酸,其裂解位点为HRRRR,属于基因型Ⅰ(类QX型);两株IBV的S1基因间核苷酸序列及其推导的氨基酸序列同源性较低,与位于同一基因型的参考毒株间同源性较高,而与中国使用的Mass型常规疫苗H120和H52之间的同源性最低,仅为75.7%~76.3%和77.1%~77.9%。本研究可为广东省IBV的流行病学调查和分子生物学研究提供参考。

关键词: 鸡传染性支气管炎病毒; 分离; 鉴定; S1基因; 序列分析

Abstract: In order to investigate the epidemiology and genetic variation of avian infectious bronchitis virus (IBV) in Guangdong province, two strains of IBV were isolated by inoculation of embryo and RT-PCR detection from diseased chickens at different farms in Zhanjiang, Guangdong province in 2013.We denoted these two strains of IBV as CK/CH/GD/ZJ10/2013 and CK/CH/GD/ZJ11/2013, respectively.Analysis of the S1 gene sequences from these two isolated strains showed that the S1 gene of CK/CH/GD/ZJ10/2013 was 1 626 bp, which encoded 542 amino acids with a cleavage site sequence of NRFRR, while the S1 gene of CK/CH/GD/ZJ11/2013 was 1620 bp, encoded 540 amino acids with a cleavage site sequence of HRRRR.Phylogenetic analysis revealed that these two isolated strains were clustered into genetic groups Ⅲ and Ⅰ(QX-like type), respectively.Homology analysis demonstrated that nucleotide and deduced amino acid sequence homologies between these two isolated strains were lower, while the homologies were higher among the same genotypes, however, the homologies were lower comparing with current vaccine strains such as H120 and H52, with which the nucleotide homologies ranged from 75.7% to 76.3% and the amino acid homologies ranged from 77.1% to 77.9%.Further analysis showed that the CK/CH/GD/ZJ10/2013 strain was formed from a recombination event between CK/CH/GX/NN11-3 and GX-NN-6.Taken together, this study provided valuable insight into prevention and control of IBV infection in Guangdong province.

Key words: avian infectious bronchitis virus; isolation; identification; S1 gene; sequence analysis

中图分类号:

S852.65

尚辉琴, 李琳, 陈瑞爱, 刘红波, 梁梅兰, 贺东生. 两株鸡传染性支气管炎病毒的分离鉴定及S1基因序列分析[J]. , 2015, 42(8): 1963-1972.

SHANG Hui-qin, LI Lin, CHEN Rui-ai, LIU Hong-bo, LIANG Mei-lan, HE Dong-sheng. Isolation,Identification and Sequence Analysis of S1 Gene of Two Strains of Avian Infectious Bronchitis Viruses[J]. , 2015, 42(8): 1963-1972.

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两株鸡传染性支气管炎病毒的分离鉴定及S1基因序列分析

Isolation,Identification and Sequence Analysis of S1 Gene of Two Strains of Avian Infectious Bronchitis Viruses

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