鸡传染性支气管炎病毒广西株N、M基因的克隆与序列分析

doi:10.16431/j.cnki.1671-7236.2015.12.002

›› 2015, Vol. 42 ›› Issue (12): 3119-3125.doi: 10.16431/j.cnki.1671-7236.2015.12.002

鸡传染性支气管炎病毒广西株N、M基因的克隆与序列分析

屈素洁, 施开创, 粟艳琼, 胡杰, 莫胜兰, 张步娴, 李军, 邹联斌

广西动物疫病预防控制中心, 南宁 530001

收稿日期:2015-07-01 出版日期:2015-12-20 发布日期:2015-12-30
通讯作者: 邹联斌 E-mail:zoulb712122@163.com
作者简介:屈素洁(1982-),女,广西南宁人,硕士,研究方向:畜禽传染病学及分子病毒学,E-mail:sujie2008abc@163.com
基金资助:
广西区水产畜牧兽医局科技项目(桂渔牧科1204935)

Cloning and Sequence Analysis of N and M Genes of Avian Infectious Bronchitis Virus Guangxi Strain

QU Su-jie, SHI Kai-chuang, SU Yan-qiong, HU Jie, MO Sheng-lan, ZHANG Bu-xian, LI Jun, ZOU Lian-bin

Guangxi Center for Animal Disease Control and Prevention, Nanning 530001, China

Received:2015-07-01 Online:2015-12-20 Published:2015-12-30

摘要/Abstract

摘要： 为研究鸡传染性支气管炎病毒(IBV)广西流行株的遗传变异情况,本研究从广西发病鸡中分离鉴定了1株IBV。参照GenBank中IBV的核苷酸序列设计2对引物,利用RT-PCR技术对分离毒株的N、M基因进行了克隆、序列测定,并与GenBank中发表的国内外参考毒株进行比对分析。结果显示,N基因序列全长为1 230 bp,编码409个氨基酸,M基因序列全长为678 bp,编码225个氨基酸。与参考毒株相比,分离株的N基因核苷酸序列同源性为87.2%~93.3%,推导的氨基酸序列同源性为90.0%~94.4%;M基因的核苷酸序列同源性为83.6%~91.0%,推导的氨基酸序列同源性为82.7%~92.9%。在遗传进化树中,本试验分离株Guangxi156株与BJ株和LX4株两个参考株位于同一个分支上,亲缘关系较近,而与其他参考株属于不同的分支,亲缘关系较远。结果表明,本试验分离株是一株新的IBV变异株。

关键词: 鸡传染性支气管炎病毒; N基因; M基因; 基因克隆; 序列分析

Abstract: To investigate genetic variation of avian infectious bronchitis virus (IBV) in Guangxi province,one strain of IBV was isolated from chicken.Two pairs of primers for amplifying the N and M genes of IBV were designed according to the sequences in GenBank.The N and M genes of the strain were amplified by RT-PCR,and they were proved to be the N and M genes of IBV by cloning,sequencing and compared with reference IBV strains published in GenBank.The results showed that the N gene from the IBV isolate consisted of 1 230 bp,coding 409 amino acids.The M gene from the IBV isolate consisted of 678 bp,coding 225 amino acids.The sequence analysis of N gene showed that it shared 87.2% to 93.3% nucleotide homologies and 90.0% to 94.4% deduced amino acid sequence homologies with IBV strains from GenBank.The M gene sequence analysis showed that it shared 83.6% to 91.0% nucleotide homologies and 82.7% to 92.9% deduced amino acid sequence homologies.The phylogenetic tree analysis showed that it was closely related to BJ and LX4 strains,and were clustered into one group;But with the distant relatives from other strains of IBV.These results suggested that the isolate was a new variant of IBV.

Key words: avian infectious bronchitis virus; N gene; M gene; gene clone; sequence analysis

中图分类号:

S852.65

屈素洁, 施开创, 粟艳琼, 胡杰, 莫胜兰, 张步娴, 李军, 邹联斌. 鸡传染性支气管炎病毒广西株N、M基因的克隆与序列分析[J]. , 2015, 42(12): 3119-3125.

QU Su-jie, SHI Kai-chuang, SU Yan-qiong, HU Jie, MO Sheng-lan, ZHANG Bu-xian, LI Jun, ZOU Lian-bin. Cloning and Sequence Analysis of N and M Genes of Avian Infectious Bronchitis Virus Guangxi Strain[J]. , 2015, 42(12): 3119-3125.

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鸡传染性支气管炎病毒广西株N、M基因的克隆与序列分析

Cloning and Sequence Analysis of N and M Genes of Avian Infectious Bronchitis Virus Guangxi Strain

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